Zijian Zhao

High-throughput single-cell screening of a cross-lineage CAR library for early-stage CAR design and candidate discovery

Hairong Jing, Dan Yuan, Bangquan Ye, Jianlin Ma et autres

Chimeric antigen receptor T cell therapy (CAR-T) has demonstrated promising efficacy in hematological malignancies, but translating that success to solid tumors remains challenging. Here, we construct a CAR library comprising approximately 1000 variants targeting prostate-specific membrane antigen by recombining transmembrane (TM), co-stimulatory, …

cn (code pays fourni par la source)

Strategic engineering of DNA aptamers as precision modulators in CRISPR-driven oncogene control

Aolin Li, Chunyan Yang, Zijian Zhao, Tao Lu et autres

OBJECTIVE: The goal of this study was to address the limited availability of intracellular protein-binding aptamers that restrict the potential of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based signal conductor systems. We aimed to develop a programmable CRISPR-SaCas9 platform using DNA aptamers …

cn (code pays fourni par la source)

Urine Liquid Biopsies via Highly Integrated Digital PCR System for Accurate Detection of Bladder Cancer (Adv. Therap. 10/2024)

Yue Zhang, Ming Xu, Zhihong Wu, Fan Yang et autres

In article 2400191, Zijian Zhao, Yanzhao Li, Yang Yu, and co-workers develop a highly accurate urine-based diagnostic tool for bladder cancer using an on-chip heating dPCR system. By integrating genetic and epigenetic biomarkers and optimizing the diagnostic model with machine learning, the …

Urine Liquid Biopsies via Highly Integrated Digital PCR System for Accurate Detection of Bladder Cancer

Yue Zhang, Ming Xu, Zhihong Wu, Fan Yang et autres

Abstract Bladder cancer (BC) is a prevalent urological tumor with high recurrence rates, requiring long‐term monitoring. Although cystoscopy is the primary diagnostic method, its invasiveness and cost hinder routine screening and follow‐up. This study aimed to develop a novel diagnostic tool utilizing …

cn (code pays fourni par la source)

SOAPTyping: an open-source and cross-platform tool for sequence-based typing for HLA class I and II alleles

Yong Zhang, Yongsheng Chen, Huixin Xu, Zijian Zhao et autres

BACKGROUND: The human leukocyte antigen (HLA) gene family plays a key role in the immune response and thus is crucial in many biomedical and clinical settings. Utilizing Sanger sequencing, the golden standard technology for HLA typing enables accurate identification of HLA alleles …

cn, dk (code pays fourni par la source)

SOAPTyping: an open-source and cross-platform tool for Sanger sequence-based typing for HLA class I and II alleles

Yong Zhang, Yongsheng Chen, Huixin Xu, Zijian Zhao et autres

ABSTRACT Summary The human leukocyte antigen (HLA) gene family plays a key role in the immune response and thus is crucial in many biomedical and clinical settings. Utilizing Sanger sequencing - the gold standard technology for HLA typing – enables accurate identification …

cn (code pays fourni par la source)