Claire A. Rinehart

Complete Genome Sequences of Mycobacteriophages TribleTrouble, TClif, Llorens and CallaLilly

Sofia Sileo, Daniela Lopez Llorens, Alaina Bowers, Trisha Chhabra et autres

155. Phages TribleTrouble, TClif, Llorens and CallaLilly have siphovirus morphology and double-stranded DNA genomes consisting of 61,665bp, 61,466bp, 59,708bp and 59,631bp, respectively. Based on gene content similarity, these phages belong to the K cluster of Mycobacterium phages. No novel genomic features were …

Writing Microbiology Resource Announcements (MRA)

Arturo Diaz, Julia Y. Lee‐Soety, Shima Chaudhary, Denise L. Monti et autres

After annotating their phage genomes, students will write a manuscript describing the phage(s) they discovered. The manuscript will be published on the pre-print repository BioRxiv, where it will be indexed and searchable via PubMed and can be readily referenced. Faculty and students …

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Genome Sequences of Cluster E Mycobacteriophages Xandras and BigBubba

Ashley Gledhill, Janelle Aguazul, Ava R Blackledge, Michael H Bucks et autres

155 from enriched soil samples. Xandras' genome length is 75,179 bp with 144 predicted protein-coding genes and two tRNAs. BigBubba's genome length is 75,006 bp with 147 predicted protein-coding genes and two tRNAs. Each genome contains a cyclic oligonucleotide sequestering protein (CBASS …

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An inclusive Research and Education Community (iREC) model to facilitate undergraduate science education reform

Denise L. Monti, Julia C. Gill, Tamarah L. Adair, Sandra D. Adams et autres

Over the last two decades, there have been numerous initiatives to improve undergraduate student outcomes in STEM. One model for scalable reform is the inclusive Research Education Community (iREC). In an iREC, STEM faculty from colleges and universities across the nation are …

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Models of classroom assessment for course-based research experiences

David I. Hanauer, Tong Zhang, Mark J Graham, Sandra D. Adams et autres

Course-based research pedagogy involves positioning students as contributors to authentic research projects as part of an engaging educational experience that promotes their learning and persistence in science. To develop a model for assessing and grading students engaged in this type of learning …

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Instructional Models for Course-Based Research Experience (CRE) Teaching

David I. Hanauer, Mark J Graham, Rachel J. Arnold, Mary A. Ayuk et autres

The course-based research experience (CRE) with its documented educational benefits is increasingly being implemented in science, technology, engineering, and mathematics education. This article reports on a study that was done over a period of 3 years to explicate the instructional processes involved …

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Genomic diversity of bacteriophages infecting Microbacterium spp

Deborah Jacobs‐Sera, Lawrence Abad, Richard M. Alvey, Kirk R. Anders et autres

The bacteriophage population is vast, dynamic, old, and genetically diverse. The genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, and replete with genes of unknown function. To further …

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Whole genome sequencing identifies an allele responsible for clear vs. turbid plaque morphology in a Mycobacteriophage

Bhavani S. Gudlavalleti, Trong H. Phung, Charles L. Barton, Allysson Becker et autres

Abstract Background Whole genome sequencing promises to revolutionize our ability to link genotypic and phenotypic variation in a wide range of model and non-model species. Results Here we describe the isolation and characterization of a novel mycobacteriophage named BGlluviae that grows on …

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Complete Genome Sequences of 44 Arthrobacter Phages

Karen K. Klyczek, Deborah Jacobs‐Sera, Tamarah L. Adair, Sandra D. Adams et autres

ABSTRACT We report here the complete genome sequences of 44 phages infecting Arthrobacter sp. strain ATCC 21022. These phages have double-stranded DNA genomes with sizes ranging from 15,680 to 70,707 bp and G+C contents from 45.1% to 68.5%. All three tail types …

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An inclusive Research Education Community (iREC): Impact of the SEA-PHAGES program on research outcomes and student learning

David I. Hanauer, Mark J Graham, Laura Betancur, Aiyana Bobrownicki et autres

Engaging undergraduate students in scientific research promises substantial benefits, but it is not accessible to all students and is rarely implemented early in college education, when it will have the greatest impact. An inclusive Research Education Community (iREC) provides a centralized scientific …

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Genome Sequences of Subcluster K5 Mycobacteriophages AlleyCat, Edugator, and Guillsminger

Rodney A. King, Tina M. Slowan-Pomeroy, Jodi E. Thomas, Tithe Ahmed et autres

ABSTRACT Bacteriophages AlleyCat, Edugator, and Guillsminger were isolated on Mycobacterium smegmatis mc 2 155 from enriched soil samples. All are members of mycobacteriophage subcluster K5, with genomes of 62,112 to 63,344 bp. Each genome contains 92 to 99 predicted protein-coding genes and …

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Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages

Karen K. Klyczek, J. Alfred Bonilla, Deborah Jacobs‐Sera, Tamarah L. Adair et autres

The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication …

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