Carmen Schalla

Spatial profiling of the spleen in mouse and human myelofibrosis reveals complement-driven immune-stromal interactions as a therapeutic target

Bérénice Dugué, Pia Wanner, Mayra L. Ruiz Tejada Segura, Mohamed Saad et autres

Splenomegaly is a defining feature of myelofibrosis, yet the contribution of splenic mesenchymal stroma to disease progression remains unclear. We combined spatial and single-nucleus transcriptomics of patient spleens with spatial and single-cell transcriptomics, as well as imaging analyses, of murine spleens to …

fr, de, nl (code pays fourni par la source)

IRF8 restrains a neutrophil alarmin program that drives inflammatory remodeling of the bone marrow niche

Rebekka K. Schneider, Anna Galyga, Mohamed Saad, Carmen Schalla et autres

de, us (code pays fourni par la source)

Oncodevelopmental plasticity of the skeleton in myeloid neoplasms

Salim Atakhanov, Ian Ghezzi, Hector Tejeda Mora, Lucas Greven et autres

Abstract Myelofibrosis in patients with myeloproliferative neoplasms (MPNs) is traditionally characterized by bone marrow fibrosis and osteosclerosis, with de novo bone formation commonly attributed to impaired osteoclast-mediated resorption. Here, we challenge this paradigm by demonstrating that a solitary clonal driver mutation simultaneously …

de, nl, ch (code pays fourni par la source)

Non-invasive detection of bone marrow fibrosis in myeloproliferative neoplasms using cell-free RNA

Mohamed Saad, Stijn N. R. Fuchs, Carmen Schalla, Katrin Götz et autres

[This corrects the article DOI: 10.1016/j.isci.2025.114325.].

Non-invasive detection of bone marrow fibrosis in myeloproliferative neoplasms using cell-free RNA

Mohamed I. Saad, Stijn N. R. Fuchs, Carmen Schalla, Katrin Götz et autres

Myeloproliferative neoplasms (MPNs), particularly with myelofibrosis (MF), involve a disrupted perivascular hematopoietic niche, ultimately leading to bone marrow fibrosis. We asked if the transcriptome in cell-free RNA (cf-RNA) from the peripheral blood of patients with MPN (with JAK2V617F mutation) can detect bone …

de, nl (code pays fourni par la source)

The Janus Face of SPAG6 : Inducing EMT in Luminal Breast Cancer Cells Amidst Widespread Expression Loss in Breast Tumours

Antonio S. Sechi, Jolein Mijnes, Sophia Villwock, Michael B. Rose et autres

Understanding the role and molecular regulation of genes associated with tumour cell motility may be informative for future cancer therapy development. Sperm-associated antigen 6 (SPAG6) gene, encoding an evolutionarily highly conserved flagellar motility protein, is regulated by promoter hypermethylation in breast cancer. …

de, in, ch (code pays fourni par la source)

Highly fluorescent hybrid nanofibers as potential nanofibrous scaffolds for studying cell-fiber interactions

Sebastian Raja, Rafaella T. Paschoalin, Idelma A. A. Terra, Carmen Schalla et autres

pl, br, de, sa (code pays fourni par la source)

Met-Signaling Controls Dendritic Cell Migration in Skin by Regulating Podosome Formation and Function

Ahmed E.I. Hamouda, Carmen Schalla, Antonio S. Sechi, Martin Zenke et autres

Signaling through the HGF receptor/Met in skin-resident Langerhans cells (LCs) and dermal dendritic cells (DCs) is essential for their emigration toward draining lymph nodes upon inflammation-induced activation. In this study, we addressed the role of Met signaling in distinct steps of LC/dermal …

de (code pays fourni par la source)

Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues

Hans Christian Probst, Patrizia Stoitzner, Lukas Amon, Ronald A. Backer et autres

This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs …

de, at, fr, il, sk, se, dk (code pays fourni par la source)

Author response for "Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues"

Hans Christian Probst, Patrizia Stoitzner, Lukas Amon, Ronald A. Backer et autres

Author response for "Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues"

Hans Christian Probst, Patrizia Stoitzner, Lukas Amon, Ronald A. Backer et autres

SPAG6 promotes cell migration and induces epithelial-to-mesenchymal transition in luminal breast cancer cells

Jolein Mijnes, Sarah Bringezu, Jonas Berger, Carmen Schalla et autres

Abstract Understanding the involvement of promoter DNA methylation changes in the development of breast cancer may be highly informative for designing more effective therapeutic treatments. We recently characterized the Sperm Associated Antigen 6 ( SPAG6 ) gene, encoding a flagellar motility protein, …

us, de (code pays fourni par la source)