Arnat Balabiyev

TARGETING THE CAPSULE OF KLEBSIELLA PNEUMONIAE WITH A CATIONIC CR3-BINDING PROTEIN ENHANCES PHAGOCYTOSIS AND PROMOTES BACTERIAL CLEARANCE AND SURVIVAL IN A MOUSE SEPSIS MODEL

Nataly Podolnikova, Iryna Klymenko, James Alagna, Cyrus Diercksmeier et autres

ABSTRACT Platelet Factor 4 (PF4), a cationic antimicrobial peptide, serves as a ligand for the myeloid-specific phagocytic receptor CR3 (Mac-1, CD11b/CD18). We previously demonstrated that recombinant dimeric PF4 (rdPF4) functions as a bacterial opsonin, enhancing phagocytosis of Gram-positive Staphylococcus aureus and facilitating …

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Control of Macrophage Fusion By Sensing the Physical Properties of Fibrin(ogen) Matrices

Nataly P. Podolnikova, Arnat Balabiyev, Woosuk S. Hur, Matthew J. Flick et autres

Macrophage fusion is a salient and unwanted characteristic of implanted biomaterials. We have recently shown that macrophage fusion requires fibrinogen, as fibrinogen-deficient mice do not support this process. We also found that this is not fibrinogen itself but rather its clotting product, …

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254 Enhancing T cell persistence in adoptive cell therapy: leveraging tumoroid-basedex-vivoassay for preclinical evaluation

Nisha R. Dhanushkodi, Abhinit Nagar, Jared Ehrhart, Arnat Balabiyev et autres

Background The duration of T cell persistence post infusion is crucial for the success of adoptive cell therapy (ACT). Despite exhibiting full functionality, insufficient autologous or engineered T cell persistence can hinder an effective anti-tumor response. Consequently, T cells’ persistence and migratory …

253 Harnessing tumoroids to advance CAR-T cell therapies

Nisha R. Dhanushkodi, Abhinit Nagar, Jared Ehrhart, Arnat Balabiyev et autres

Background Advancements in CAR-T cell therapies for solid tumors require a robust evaluation framework to assess efficacy in the complex tumor microenvironment, unravel operational mechanisms, and identify prognostic biomarkers for treatment response. Existing preclinical models, such as patient-derived xenografts and traditional organoids, …

500 Assessing the efficacy of immunotherapies using Nilogen Oncosystems’ 3D-EXplore platform: a focus on PD-1/PD-L1 inhibitors in NSCLC tumors

Abhinit Nagar, Jared Ehrhart, Arnat Balabiyev, Nisha R. Dhanushkodi et autres

Background Despite the notable progress achieved by PD-1/PD-L1 immunotherapies in cancer treatment, their effectiveness in clinical trials often faces challenges. Models commonly used for pre-clinical evaluation of PD-1/PD-L1 immunotherapies often are biased due to their inability to mimic the intricate tumor microenvironment. …

121 Exploring therapeutic potential: insights from drug penetration and targeting using Nilogen’s ex-vivo 3D-EXpress tumoroid platform

Abhinit Nagar, Jared Ehrhart, Arnat Balabiyev, Nisha R. Dhanushkodi et autres

Background The effectiveness of therapeutics relies on their ability to penetrate and target solid tumors, often hindered by complex tumor-stroma architecture. Labeling therapeutics with fluorochromes aids in visualizing infiltration, binding, and internalization kinetics, enhancing our understanding of mechanisms of action for a …

816 Predictive modeling of immune-related adverse events using tumoroids

Arnat Balabiyev, Abhinit Nagar, Jared Ehrhart, Nisha R. Dhanushkodi et autres

Background Immunotherapy has revolutionized cancer treatment, yet the emergence of immune-related adverse events (irAEs) poses significant challenges to its widespread adoption. Predicting and managing irAEs is still a critical aspect of patient care. Tumoroids which retain the endogenous tumor microenvironment offer a …

82 Advancing cancer therapy: high-content imaging for precise tumor cell killing analysis in patient-derived tumoroids

Seth Currlin, Abhinit Nagar, Jared Ehrhart, Arnat Balabiyev et autres

Background Advancing cancer research and therapeutic development relies on effective tumor cell killing (TCK) analysis, particularly during the critical preclinical phase where accurate evaluation platforms are indispensable. Conventional methods, such as cell lines or primary cultures, often fail to replicate the complex …

Fibrin polymer on the surface of biomaterial implants drives the foreign body reaction

Arnat Balabiyev, Nataly P. Podolnikova, Jacquelyn Kilbourne, D. Page Baluch et autres

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The Cis Interaction of CD47 with Integrin Mac‐1 (α M β 2 , CD11b/CD18) Regulates Macrophage Responses

Nataly P. Podolnikova, Arnat Balabiyev, Tatiana P. Ugarova

CD47 is a widely expressed cell surface receptor that has originally been identified as an integrin‐associated protein. In immune cells, CD47 has been shown to associate in cis with several integrins of the β 1 and β 3 subfamilies and the one …

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Fibrin Polymer on the Surface of Biomaterial Implants Drives the Foreign Body Reaction

Arnat Balabiyev, Nataly P. Podolnikova, Jacquelyn Kilbourne, D. Page Baluch et autres

ABSTRACT Implantation of biomaterials and medical devices in the body triggers the foreign body reaction (FBR) which is characterized by macrophage fusion at the implant surface leading to the formation of foreign body giant cells and the development of the fibrous capsule …

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Transition of podosomes into zipper-like structures in macrophage-derived multinucleated giant cells

Arnat Balabiyev, Nataly P. Podolnikova, Aibek Mursalimov, David Lowry et autres

, and other components of podosomes are present in ZLSs. Macrophages deficient in WASp or Cdc42, two key molecules involved in actin core organization in podosomes, as well as cells treated with the inhibitors of the Arp2/3 complex, failed to form ZLSs. …

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