Fengjie Cui

Semi-Rational Design of Lignin Peroxidase for Enhanced Activity and Application in Wheat Straw Lignin Degradation

Yong Chen, Na Shi, Anjun Li, De Cheng et autres

Abstract Lignin peroxidase (LiP) is a key bottleneck in wheat straw lignin degradation due to its limited catalytic efficiency. In this study, a lignin peroxidase from Phanerochaete chrysosporium (PcLiP; GenBank accession no. X15599.1) was engineered using a semirational design strategy. Seven candidate …

cn, se (code pays fourni par la source)

Deciphering the genetic background of an industrial 2-ketogluconic acid-producing strain Pseudomonas plecoglossicida JUIM01 using whole-genome sequencing

Lulu Li, Lei Sun, Xinyi Zan, Fengjie Cui et autres

2-Ketogluconic acid (2KGA) is an important precursor for the food antioxidant erythorbic acid, currently produced via microbial fermentation using Pseudomonas species. To facilitate the genetic improvement of production strains, the complete genome of an industrial 2KGA producer P. plecoglossicida JUIM01 was sequenced …

cn (code pays fourni par la source)

Substrate-Binding Site Engineering of Thermomyces dupontii Lipase (TDL) to Improve Selectivity for the Synthesis of Functional sn -1,3-Diacylglycerol

Yixin Zhang, JianYong Lei, Lei Sun, YuFeng Qian et autres

Diacylglycerol (DAG) oils have shown unique health functions, including preventing weight gain, lowering serum triacylglycerol (TAG) levels, and improving glucose metabolism. sn -1,3-DAG produced by esterification, hydrolysis, and glycerolysis using regioselective microbial lipases is regarded as the sole compound contributing to DAG’s …

cn, ru (code pays fourni par la source)

Phosphoproteomic Insights into the Metabolic Reprogramming of Cell Growth and Lipid Accumulation in Mucor circinelloides

Xinyi Zan, Jun Shen, Y. F. Wang, Lina Zhao et autres

Mucor circinelloides is a model oleaginous fungus for studying microbial lipid accumulation, yet the post-translational mechanisms coordinating growth and lipid biosynthesis remain poorly understood. Here, we present the first systematic quantitative phosphoproteomic analysis of M. circinelloides across its life cycle, encompassing spores …

cn, us, fr (code pays fourni par la source)

Chitin Synthases in Fungi: A Comprehensive Review of Classification, Catalytic Mechanism, and Functional Roles

Lin Xu, Ting-Ting Wen, Cai-Yu Chen, L. Sun et autres

Fungi contain the most complex chitin synthase family, which contributes to chitin biosynthesis and cell wall rigidity. In the past decades, their functions involved in morphological diversity, viability, and virulence of fungi have been well investigated using typical molecular methods such as …

cn, si (code pays fourni par la source)

Forecasting Industrial Carbon Peaking and Exploring Emission Reduction Pathways at the Metropolitan Scale: A Multi-Scenario STIRPAT Analysis of the Hangzhou Metropolitan Area

Fengjie Cui, Zhoukai Chen, Xiaoan Li, Xiangdong Xue et autres

The rapid development of industry has led to intensive energy and resource consumption, increasing carbon emissions. As key areas for carbon control, metropolitan regions play an essential role in China’s urbanization and regional development, yet research on predicting industrial carbon emissions remains …

cn, dk (code pays fourni par la source)

Functional Characterization of a β-1,3-Glucanosyltransferase CmGel4 in Cordyceps militaris Using a Precise CRISPR-Cas9 Genome-Editing System

Ting-Ting Wen, Xu Lin, Rui Jin, Zhiyu Liu et autres

Cordyceps militaris polysaccharides, especially β-glucans, have presented significant antitumor, hypoglycemic, and immunomodulatory activities. However, the enzymes involved in the branching formation of C. militaris β-glucans remain to be elucidated. In the present study, a 1.69-kb β-1,3-glucanosyltransferase CmGel4 gene putatively involved in β-glucan …

cn, si, cz (code pays fourni par la source)

Unlocking Kluyveromyces marxianus for efficient protein expression and single-cell protein production

Fengjie Cui, Yu Cao, Xuan Lou, Jun O. Liu et autres

cn (code pays fourni par la source)

The Role of Glyceraldehyde-3-Phosphate Dehydrogenase in 2-Ketogluconic Acid Industrial Production Strain Pseudomonas plecoglossicida JUIM01

Lei Sun, Qian-Nan Zhang, Lulu Li, Lei Zhang et autres

The full-length gapA gene (1002 bp) was cloned from Pseudomonas plecoglossicida JUIM01, an industrial strain used for 2-ketogluconic acid (2KGA) production. The protein encoded by gapA (designated Gap) was predicted to be a canonical NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase that catalyzes the interconversion between …

cn (code pays fourni par la source)

Characterization and antifungal target evaluation of cell wall polysaccharides from Botrytis cinerea mycelium

Lijuan Meng, Dongmei Li, Zi‐Ying Zhang, Jin-Long Xu et autres

cn, kr (code pays fourni par la source)

Targeted quercetin extraction from cherry and strawberry juices via chitosan-modified macroporous monolith

Umar Zeb, Shakeel Zeb, Tariq Aziz, Abdul Haleem et autres

cn (code pays fourni par la source)

Caspofungin-lavender conjugates halt Alternaria rot in peaches: Unravelling antifungal mechanisms in-vitro, in-vivo, and in-silico

Syed Asim Shah Bacha, Shashi Kiran, Ting-Ting Wen, Asif Ali Khan et autres

cn, ae, Égypte (code pays fourni par la source)