Christian Much

Distinct specificity and functions of PRC2 subcomplexes in human stem cells and cardiac differentiation

Christian Much, Sandy M. Rajkumar, Liming Chen, Aravind R. Gade et autres

us (code pays fourni par la source)

Macromolecular interactions dictate Polycomb-mediated epigenetic repression

Christian Much, Sandy M. Rajkumar, Liming Chen, Aravind R. Gade et autres

Abstract The dynamic regulation of epigenetic states relies on complex macromolecular interactions. PRC2, the methyltransferase complex responsible for depositing H3K27me3, interacts with distinct accessory proteins to form the mutually exclusive subcomplexes PHF1-PRC2.1, MTF2-PRC2.1, PHF19-PRC2.1, and PRC2.2. The functions of these subcomplexes are …

us (code pays fourni par la source)

The temporal dynamics of lncRNA Firre-mediated epigenetic and transcriptional regulation

Christian Much, Erika L. Lasda, Isabela Tiemy Pereira, Tenaya K. Vallery et autres

Numerous studies have now demonstrated that lncRNAs can influence gene expression programs leading to cell and organismal phenotypes. Typically, lncRNA perturbations and concomitant changes in gene expression are measured on the timescale of many hours to days. Thus, we currently lack a …

us (code pays fourni par la source)

T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation

Alexandro Landshammer, Adriano Bolondi, Helene Kretzmer, Christian Much et autres

Long non-coding RNAs (lncRNAs) have emerged as fundamental regulators in various biological processes, including embryonic development and cellular differentiation. Despite much progress over the past decade, the genome-wide annotation of lncRNAs remains incomplete and many known non-coding loci are still poorly characterized. …

de, us, cn (code pays fourni par la source)

Author response: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation

Alexandro Landshammer, Adriano Bolondi, Helene Kretzmer, Christian Much et autres

Long non-coding RNAs (lncRNAs) have emerged as fundamental regulators in various biological processes, including embryonic development and cellular differentiation.Despite much progress over the past decade, the genome-wide annotation of lncRNAs remains incomplete and many known non-coding loci are still poorly characterized.Here, we …

de, us, cn (code pays fourni par la source)

Abstract IA010: The lncRNA Firre functions as a transcriptional activator from a distance

John L. Rinn, Christian Much, Erika L. Lasda, Jordan P. Lewandowski et autres

Abstract There are currently over 16,000 long-noncoding RNAs (lncRNAs) annotated in mammalian genomes, a number on par with protein-coding genes. Numerous studies have demonstrated that lncRNAs can influence gene expression programs, but lack the temporal resolution to identify the immediate regulatory events. …

us (code pays fourni par la source)

Structural and functional basis of mammalian microRNA biogenesis by Dicer

David Zapletal, Eliška Táborská, Josef Pasulka, Radek Malı́k et autres

MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucleases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is specifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a …

cz, gb, it, hr, at, ru (code pays fourni par la source)

Discovery and characterization of LNCSOX17 as an essential regulator in human endoderm formation

Alexandro Landshammer, Adriano Bolondi, Helene Kretzmer, Christian Much et autres

ABSTRACT Long non-coding RNAs (lncRNAs) have emerged as fundamental regulators in various biological processes, including embryonic development and cellular differentiation. Despite much progress over the past decade, the genome-wide annotation of lncRNAs remains incomplete and many known non-coding loci are still poorly …

de, us, cn (code pays fourni par la source)

The temporal dynamics of lncRNA Firre -mediated epigenetic and transcriptional regulation

Christian Much, Erika L. Lasda, Isabela Tiemy Pereira, Tenaya K. Vallery et autres

Abstract Numerous studies have now demonstrated that lncRNAs can influence gene expression programs leading to cell and organismal phenotypes. Typically, lncRNA perturbations and concomitant changes in gene expression are measured on the timescale of many hours to days. Thus, we currently lack …

us (code pays fourni par la source)

Molecular basis of indispensable accuracy of mammalian miRNA biogenesis

D. Zapletal, Eliška Táborská, Josef Pasulka, Radek Malı́k et autres

Abstract Mammalian Dicer is the gatekeeper into the essential gene-regulating miRNA pathway. What is committing mammalian Dicer to the miRNA pathway remains unknown. We report that Dicer’s highly conserved DExD/H helicase domain is the key structural element supporting accurate miRNA biogenesis. While …

cz, gb, hr (code pays fourni par la source)

Evolutionary divergence of Firre localization and expression

Christian Much, Michael J. Smallegan, Taeyoung Hwang, Skylar D. Hanson et autres

Long noncoding RNAs (lncRNAs) are rapidly evolving and thus typically poorly conserved in their sequences. How these sequence differences affect the characteristics and potential functions of lncRNAs with shared synteny remains unclear. Here we show that the syntenically conserved lncRNA Firre displays …

us, de (code pays fourni par la source)

NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells

Azzurra Codino, Tomasz W. Turowski, Louie N. van de Lagemaat, Ivayla Ivanova et autres

mouse allele and applied the highly stringent cross-linking and analysis of cDNAs to define NANOS2 RNA occupancy in SSC lines. NANOS2 recognizes the AUKAAWU consensus motif, mostly found in the 3' untranslated region of defined messenger RNAs (mRNAs). We find that NANOS2 …

gb, de (code pays fourni par la source)