Nathan G. Schoepp

Characterizing cell interactions at scale with made-to-order droplet ensembles (MODEs)

Justin L. Madrigal, Nathan G. Schoepp, Linfeng Xu, Codian S. Powell et autres

Cell-cell interactions are important to numerous biological systems, including tissue microenvironments, the immune system, and cancer. However, current methods for studying cell combinations and interactions are limited in scalability, allowing just hundreds to thousands of multicell assays per experiment; this limited throughput …

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Abstract 133: Micro-environment on demand (MOD): A versatile technology for cognate T-cell receptor - neoantigen discovery

Maithreyan Srinivasan, Justin L. Madrigal, Nathan G. Schoepp, Russell H. Cole

Abstract NCI estimates that cancer will be the leading cause of death in 2030, worldwide. Checkpoint inhibitors and adoptive cell therapies (ACTs) cost up to ~$2 million/patient and have shown durable responses in a few patients while many patients experience life-threatening side-effects. …

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Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae

Nathan G. Schoepp, Eric J. Liaw, Alexander Viloria Winnett, Emily S. Savela et autres

The rise in carbapenem-resistant Enterobacteriaceae (CRE) infections has created a global health emergency, underlining the critical need to develop faster diagnostics to treat swiftly and correctly. Although rapid pathogen-identification (ID) tests are being developed, gold-standard antibiotic susceptibility testing (AST) remains unacceptably slow …

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Surfactant-enhanced DNA accessibility to nuclease accelerates phenotypic β-lactam antibiotic susceptibility testing of Neisseria gonorrhoeae

Emily S. Savela, Nathan G. Schoepp, Matthew M. Cooper, Justin C. Rolando et autres

Rapid antibiotic susceptibility testing (AST) for Neisseria gonorrhoeae (Ng) is critically needed to counter widespread antibiotic resistance. Detection of nucleic acids in genotypic AST can be rapid, but it has not been successful for β-lactams (the largest antibiotic class used to treat …

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P662 Cell envelope damage ofN. gonorrhoeaeafter 15-min beta-lactam exposure enables rapid antimicrobial susceptibility testing

Emily S. Savela, Nathan G. Schoepp, Justin C. Rolando, Olusegun O. Soge et autres

Background Designing diagnostic tools to perform phenotypic antimicrobial susceptibility testing (AST) at the point-of-care (POC) is a vital step in tackling the global threat of antimicrobial resistance. Gonorrhea infections with resistance to the first-line dual therapy have already emerged, highlighting the impending …

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Phenotypic Antimicrobial Susceptibility Testing Based on Nucleic Acid Analysis

Nathan G. Schoepp

Antimicrobial resistance (AMR) is one of the most widely recognized threats to global health, and one that continues to grow as new mechanisms of resistance evolve and resistant pathogens spread. Antibiotics are a cornerstone of modern medicine, but their misuse and overuse …

Real-Time, Digital LAMP with Commercial Microfluidic Chips Reveals the Interplay of Efficiency, Speed, and Background Amplification as a Function of Reaction Temperature and Time

Justin C. Rolando, Erik Jue, Nathan G. Schoepp, Rustem F. Ismagilov

Real-time, isothermal, digital nucleic acid amplification is emerging as an attractive approach for a multitude of applications including diagnostics, mechanistic studies, and assay optimization. Unfortunately, there is no commercially available and affordable real-time, digital instrument validated for isothermal amplification; thus, most researchers …

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RNA markers enable phenotypic test of antibiotic susceptibility in Neisseria gonorrhoeae after 10 minutes of ciprofloxacin exposure

Tahmineh Khazaei, Jacob T. Barlow, Nathan G. Schoepp, Rustem F. Ismagilov

Antimicrobial-resistant Neisseria gonorrhoeae is an urgent public-health threat, with continued worldwide incidents of infection and rising resistance to antimicrobials. Traditional culture-based methods for antibiotic susceptibility testing are unacceptably slow (1-2 days), resulting in the use of broad-spectrum antibiotics and the further development …

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Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples

Nathan G. Schoepp, Travis S. Schlappi, Matthew S. Curtis, Slava S. Butkovich et autres

directly from clinical urine samples in less than 30 min. With further development for additional pathogens, antibiotics, and sample types, rapid digital AST (dAST) could enable rapid clinical decision-making, improve management of infectious diseases, and facilitate antimicrobial stewardship.

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Corrigendum: Digital Quantification of DNA Replication and Chromosome Segregation Enables Determination of Antimicrobial Susceptibility after only 15 Minutes of Antibiotic Exposure

Nathan G. Schoepp, Eugenia M. Khorosheva, Travis S. Schlappi, Matthew S. Curtis et autres

In the “Antibiotic exposure time course experiments” section of the “Experimental Section” of the Supporting Information for this Communication, “early logarithmic” should read “early stationary (OD600 3.1–6.9).” Also, the authors do not recommend the use of Epicentre QuickExtract RNA Extraction Solution for …

Berichtigung: Digital Quantification of DNA Replication and Chromosome Segregation Enables Determination of Antimicrobial Susceptibility after only 15 Minutes of Antibiotic Exposure

Nathan G. Schoepp, Eugenia M. Khorosheva, Travis S. Schlappi, Matthew S. Curtis et autres

Im Abschnitt “Antibiotic exposure time course experiments” des Experimentalteils in den Hintergrundinformationen zu dieser Zuschrift muss “early logarithmic” durch “early stationary (OD600 3.1–6.9)” ersetzt werden. Die Autoren raten außerdem von der Verwendung der “Epicentre QuickExtract RNA Extraction Solution” für Pathogene ab, da …

Evaluation of phenotype stability and ecological risk of a genetically engineered alga in open pond production

Shawn J. Szyjka, Shovon Mandal, Nathan G. Schoepp, Briana M. Tyler et autres

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