Clare M. Waterman

CD44 restricts EGFR mobility to polarize cytoskeletal signalling modules driving bleb-based migration

Ankita Jha, Ankit Chandra, Payam E. Farahani, Jared E. Toettcher et autres

Cells under high confinement migrate efficiently in low-adhesion environments by forming stable, polarized, hydrostatic pressure-driven leader blebs. Here we investigated the basis of polarized bleb morphology in metastatic melanoma cells migrating under low-adhesive and highly confined microenvironments. Using high-resolution live imaging, molecular …

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Lamin B receptor upregulation in metastatic melanoma causes nuclear envelope fragility in confined migration during cancer invasion

Michelle A. Baird, Cayla E Jewett, Daniela A. Malide, Lisa E. Kratz et autres

Cancer cells migrate through regions of tissue confinement during metastasis, causing nuclear envelope (NE) rupture, which generates heritable DNA damage and disease aggression. We sought to determine if NE rupture was intrinsic to malignant transformation and seek a mechanistic cause. We found …

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Intrinsic mechanotransduction during apical constriction licenses lineage competence in pluripotent stem cells

Mehdi S. Hamouda, Céline Labouesse, George W. Wylde, Daniel Yamamoto et autres

To acquire the capacity for multi-lineage differentiation, pluripotent stem cells must undergo a transition from naïve pluripotency to lineage competency. This transition requires epithelialization and changes in nuclear architecture. We sought to determine whether the cell and tissue mechanics intrinsic to epithelialization …

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CD44 and Ezrin restrict EGF receptor mobility to generate a novel spatial arrangement of cytoskeletal signaling modules driving bleb-based migration

Ankita Jha, Ankit Chandra, Payam E. Farahani, Jared E. Toettcher et autres

Cells under high confinement form highly polarized hydrostatic pressure-driven, stable leader blebs that enable efficient migration in low adhesion, environments. Here we investigated the basis of the polarized bleb morphology of metastatic melanoma cells migrating in non-adhesive confinement. Using high-resolution time-lapse imaging …

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Blebology: principles of bleb-based migration

Juan Manuel GARCIA ARCOS, Ankita Jha, Clare M. Waterman, Matthieu Piel

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Lamin B Receptor Upregulation in Metastatic Melanoma Causes Cholesterol-Mediated Nuclear Envelope Fragility

Michelle A. Baird, Robert S Fischer, Cayla E Jewett, Daniela A. Malide et autres

Abstract Metastatic cancer cells migrate through regions of tissue confinement, causing nuclear envelope (NE) rupture and heritable DNA damage. We discovered that cells from multiple cancers have increased NE fragility in confinement and transcriptional upregulation of nuclear genes compared to benign counterparts. …

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NLRP3 is essential for neutrophil polarization and chemotaxis in response to leukotriene B4 gradient

Stijn Van Bruggen, P.A. Jarrot, Eline Thomas, Casey E. Sheehy et autres

Neutrophil recruitment to sites of infection and inflammation is an essential process in the early innate immune response. Upon activation, a subset of neutrophils rapidly assembles the multiprotein complex known as the NLRP3 inflammasome. The NLRP3 inflammasome forms at the microtubule organizing …

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Single-molecule characterization of subtype-specific β1 integrin mechanics

Myung Hyun Jo, Jing Li, Valentin Jaumouillé, Yuxin Hao et autres

Although integrins are known to be mechanosensitive and to possess many subtypes that have distinct physiological roles, single molecule studies of force exertion have thus far been limited to RGD-binding integrins. Here, we show that integrin α4β1 and RGD-binding integrins (αVβ1 and …

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Whose Agenda is It? Navigating the Politics of Setting the Research Agenda in Education Research-Practice Partnerships

Joanna L. Meyer, Clare M. Waterman, George A. Coleman, Michael J. Strambler

In this article, we provide insight into sharing power and balancing practitioner and researcher priorities during the process of establishing a research agenda for a research-practice partnership (RPP). We draw on the literature about effective collaboration within RPPs to identify concepts and …

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Whose agenda is it? Navigating the politics of setting the research agenda in education research-practice partnerships

Joanna L. Meyer, Clare M. Waterman, George A. Coleman, Michael J. Strambler

In this article, we provide insight into sharing power and balancing practitioner and researcher priorities during the process of establishing a research agenda for a research-practice partnership (RPP). We draw on the literature about effective collaboration within RPPs to identify concepts and …

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Subtype-specific single β1 integrin mechanics for activation, mechanotransduction and cytoskeleton remodeling

Myung Hyun Jo, Jing Li, Valentin Jaumouillé, Yuxin Hao et autres

Abstract Although integrins are known to be mechano-sensitive and to possess many subtypes that have distinct physiological roles, single molecule studies of force exertion have thus far been limited to RGD-binding integrins. Here, we show that integrin α4β1 and RGD-binding integrins (αVβ1 …

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MARK2 regulates directed cell migration through modulation of myosin II contractility and focal adhesion organization

Ana M. Pasapera, Sarah M. Heissler, Masumi Eto, Yukako Nishimura et autres

Cancer cell migration during metastasis is mediated by a highly polarized cytoskeleton. MARK2 and its invertebrate homolog Par1B are kinases that regulate the microtubule cytoskeleton to mediate polarization of neurons in mammals and embryos in invertebrates. However, the role of MARK2 in …

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