Stanislav Sokolenko

Multi-Omics Network Integration Reveals Cross-Domain Molecular Correlates of Fetal Bovine Serum Grade

Akila Wijerathna‐Yapa, Arslan Hamid, Stanislav Sokolenko

Abstract Fetal bovine serum (FBS) is the dominant supplement in mammalian cell culture, yet its quality grades are typically treated as fixed, defined inputs despite a poorly characterized molecular basis. We present a multi-omics meta-analysis integrating original proteomics, peptidomics, metabolomics, and lipidomics …

ca, de (code pays fourni par la source)

Comprehensive Characterization of Peptides in Plant and Yeast-Based Hydrolysates for Cell Culture Media Applications

Kathy Sharon Isaac, Michelle Combe, Stanislav Sokolenko

This repository contains supplementary data for the publication Comprehensive Characterization of Peptides in Plant and Yeast-Based Hydrolysates for Cell Culture Media Applications. It includes peptidomics data of four plant-based (pea, cotton, soy, and wheat) and five different yeast-based hydrolysate products obtained via …

ca (code pays fourni par la source)

Comprehensive Characterization of Peptides in Plant and Yeast-Based Hydrolysates for Cell Culture Media Applications

Kathy Sharon Isaac, Michelle Combe, Stanislav Sokolenko

This repository contains supplementary data for the publication Comprehensive Characterization of Peptides in Plant and Yeast-Based Hydrolysates for Cell Culture Media Applications. It includes peptidomics data of four plant-based (pea, cotton, soy, and wheat) and five different yeast-based hydrolysate products obtained via …

ca (code pays fourni par la source)

Uncovering the small-molecule landscape of fetal bovine serum: Integrated untargeted metabolomics and lipidomics identify compositional programs in three commercial grade products

Akila Wijerathna-Yapa, Stanislav Sokolenko

Fetal bovine serum (FBS) is foundational to mammalian cell culture, yet the small-molecule composition of commercial grades—and its functional implications remain poorly defined. We performed integrated, untargeted LC–MS metabolomics and lipidomics on Low-, Medium-, and High-grade FBS (Thermo Fisher Scientific), and PCA …

ca (code pays fourni par la source)

Protein hydrolysates in cell culture: Toward multi‐omics characterization

Michelle Combe, Brandon M Wrage, Angel Varela‐Rohena, Stanislav Sokolenko

The high cost of conventional cell culture media has driven demand for sustainable, cost-effective serum-free formulations in biopharmaceutical manufacturing and cultivated meat production. Although plant- and yeast-derived hydrolysates are generally considered to be promising additives, significant variability in raw materials and processing …

ca, us (code pays fourni par la source)

What Is in Fetal Bovine Serum Used for Cell Culture? Integrated Proteomics and Peptidomics Reveal Grade-Dependent Molecular Profiles

Akila Wijerathna-Yapa, Stanislav Sokolenko

Fetal bovine serum (FBS) is a widely used but poorly defined mammalian cell culture supplement, introducing substantial batch-to-batch and grade-specific variability that compromises experimental reproducibility. We performed integrative proteomics and peptidomics profiling of three commercially available FBS grades using data-independent acquisition (DIA) …

ca, by (code pays fourni par la source)

Assessment of engineering graduate attributes: A case study on modeling and analyzing heterogeneous data

Kathy Sharon Isaac, Andrew J. Furlong, Stanislav Sokolenko

Graduate attributes (GAs) are a required component for accreditation of undergraduate engineering programs in Canada. Similar to ABET student outcomes in the United States, they attempt to quantify a broad range of technical and non-technical skills that engineers are expected to possess …

ca (code pays fourni par la source)

Biallelic ACER3 Variants Cause Infantile- and Early-Childhood-Onset Neurodegeneration with Leukodystrophy

Rauan B. Kaiyrzhanov, Ruijuan Xu, Mina Zamani, Robert D. Healey et autres

kz, us, gb, fr, Égypte, es, ca, in, ch, ir, tr, de, cl, no, mx, pl, nl, sa, Tunisie (code pays fourni par la source)

Complete Mass Spectrometry Dataset and Analysis Files for Metabolomics Characterization of Commercial Fetal Bovine Serum

Akila Wijerathna-Yapa, Stanislav Sokolenko

ca (code pays fourni par la source)

Complete Mass Spectrometry Dataset and Analysis Files for Metabolomics Characterization of Commercial Fetal Bovine Serum

Akila Wijerathna-Yapa, Stanislav Sokolenko

ca (code pays fourni par la source)

Neurodevelopmental disease-causing variants in choline kinase CHKA gene couple phosphatidylcholine synthesis to oxidative stress damage and disease etiology

Mahtab Tavasoli, Mariam Alkandari, Gabriel G. Dorighello, Michael McPhee et autres

Biallelic variants in CHKA, which encodes the first enzyme in the CDP-choline pathway for the synthesis of phosphatidylcholine, cause an inherited disorder characterized by epilepsy, microcephaly, and intellectual disability. How a deficiency in CHKA activity manifests these neurological symptoms is poorly understood. …

ca, gb, in, Égypte (code pays fourni par la source)

Complete Mass Spectrometry Dataset and Analysis Files for Lipidomics Characterization of Commercial Fetal Bovine Serum

Akila Wijerathna-Yapa, Stanislav Sokolenko

ca (code pays fourni par la source)