Xian-Ge Sun

Low‐Concentration Enhancement Effect: Enabling Sensitive Detection of EML4 ‐ ALK Variants via Multiplex RT ‐ MIRA

Xiaodong Ren, Luo Xi-yao, Jin-Mi Li, Xian-Ge Sun et autres

OBJECTIVE: ALK fusion variant detection is essential for guiding targeted therapy in non-small cell lung cancer (NSCLC). The emerging reverse transcription multi-enzyme isothermal rapid amplification (RT-MIRA) technology exhibits substantial potential in molecular diagnostics, enabling efficient variant profiling. However, its performance and optimization …

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Electrical characterization of single tumor cells with high accuracy using a low-cost microfluidic impedance cytometry

Hong Wang, Xiaodong Ren, Wei-Long Kong, Xian-Ge Sun et autres

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Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples

Jin Li, Mei-Yun Shang, Shaoli Deng, Min Li et autres

Bloodstream infection (BSI) caused by bacteria is highly pathogenic and lethal, and easily develops whole-body inflammatory state. Immediate identification of disease-causing bacteria can improve patient prognosis. Traditional testing methods are not only time-consuming, but such tests are limited to laboratories. Recombinase polymerase …

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Rapid Capture of DNA from Human Urine Using Chitosan-Coated Nylon Membrane and Its Application

Xiaodong Ren, Xian-Ge Sun, Ning Su, Qing Yu Huang

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Rapid detection of EGFR mutation in CTCs based on a double spiral microfluidic chip and the real-time RPA method

Wenman Li, Xiaodong Ren, Yuzhu Jiang, Ning Su et autres

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Centrifugal microfluidic-based multiplex recombinase polymerase amplification assay for rapid detection of SARS-CoV-2

Ruoxu Li, Ning Su, Xiaodong Ren, Xian-Ge Sun et autres

The COVID-19 pandemic has spread worldwide, and rapid detection of the SARS-CoV-2 virus is crucial for infection surveillance and epidemic control. This study developed a centrifugal microfluidics-based multiplex reverse transcription recombinase polymerase amplification (RT-RPA) assay for endpoint fluorescence detection of the E, …

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Isothermal exponential amplification reactions triggered by circular templates (cEXPAR) targeting miRNA

Xuemei Qu, Xiaodong Ren, Ning Su, Xian-Ge Sun et autres

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Advances in liquid biopsy–based markers in NSCLC

Xiaodong Ren, Ning Su, Xian-Ge Sun, Wenman Li et autres

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Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples

Jin Li, Mei-Yun Shang, Shaoli Deng, Min Li et autres

Abstract Bloodstream infection (BSI) caused by bacteria is highly pathogenic and lethal, and easily develops whole-body inflammatory state. Immediate identification of disease-causing bacteria can improve patient prognosis. Traditional testing methods are not only time-consuming, but such tests are limited to laboratories. Recombinase …

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Hydrological Response and Ecological Flow Optimization in Water Diversion Area of Inter-basin Water Diversion Project

Tao Bai, Xian-Ge Sun, Jian Wei, Lianzhou Wu

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A Resistance-Based Microfluidic Chip for Deterministic Single Cell Trapping Followed by Immunofluorescence Staining

Xian-Ge Sun, Bowen Li, Wenman Li, Xiaodong Ren et autres

Microchips are fundamental tools for single-cell analysis. Although various microfluidic methods have been developed for single-cell trapping and analysis, most microchips cannot trap single cells deterministically for further analysis. In this paper, we describe a novel resistance-based microfluidic chip to implement deterministic …

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An acceptable methodological evaluation method of real-time fluorescent RT-PCR targeting SARS-CoV-2

Xiaodong Ren, Qi-Mei Tang, Min Chen, Qian Huang et autres

Abstract Background: it is necessary to evaluate real-time fluorescent reverse-transcription PCR (RT-PCR) methods to detect the nucleic acids of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: considering lack of positive specimens in some particular locations in China, the specimens from healthy …

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