Michaela Helble

Nanoparticle Immunoadjuvant Complexes Augment Germinal Center Responses to Vaccination

Nicholas J. Tursi, Colby J. Agostino, Jinwei Huang, Toshitha Kannan et autres

Vaccine approaches capable of eliciting enhanced germinal center (GC) responses would result in improved protective humoral immunity against infectious diseases. Here, we investigate whether a cytokine can be scaffolded onto a self-assembling nanoparticle immunogen to enhance antigen-specific GC responses and B cell …

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Structural engineering of stabilized, expanded epitope nanoparticle vaccines for HPV

Michaela Helble, Xizhou Zhu, Pratik Sanjivkumar Bhojnagarwala, Kevin Liaw et autres

Oncogenic forms of HPV account for 4.5% of the global cancer burden worldwide. This includes cervical, vaginal, vulvar, penile, and anal cancers, as well as head and neck cancers. As such, there is an urgent need to develop effective therapeutic vaccines to …

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Structure and sequence engineering approaches to improve in vivo expression of nucleic acid-delivered antibodies

Michaela Helble, Jacqueline D. Chu, Abigail Rose Trachtman, Alana Huynh et autres

Monoclonal antibodies are an important class of biologics with over 160 Food and Drug Administration/European Union-approved drugs. A significant bottleneck to global accessibility of recombinant monoclonal antibodies stems from complexities related to their production, storage, and distribution. Recently, gene-encoded approaches such as …

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Nanoparticle immunoadjuvant complexes augment antigen-specific germinal center responses to enhance humoral immunity

Nicholas J. Tursi, Toshitha Kannan, Jennifer A. Londregan, Katlyn Lederer et autres

Abstract An alluring strategy for improving protective humoral immunity against infectious diseases would be to directly target the germinal center (GC). Robust GC responses are associated with high titer and high affinity antibody responses. Despite advances in formulation strategies and vaccine design, …

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Structure and sequence engineering of nucleic acid-delivered antibodies and nanoparticle vaccines

Michaela Helble

New nucleic-acid delivery technologies, such as DNA delivery, have recently come to the forefront of scientific advances. As this field is relatively new, the parameters and mechanisms that guide successful and potent DNA immunotherapies still require further elucidation. DNA delivery of monoclonal …

Engineered antibody cytokine chimera synergizes with DNA-launched nanoparticle vaccines to potentiate melanoma suppression in vivo

Nicholas J. Tursi, Ziyang Xu, Michaela Helble, Susanne N. Walker et autres

Cancer immunotherapy has demonstrated great promise with several checkpoint inhibitors being approved as the first-line therapy for some types of cancer, and new engineered cytokines such as Neo2/15 now being evaluated in many studies. In this work, we designed antibody-cytokine chimera (ACC) …

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SARS-CoV-2 Assays To Detect Functional Antibody Responses That Block ACE2 Recognition in Vaccinated Animals and Infected Patients

Susanne N. Walker, Neethu Chokkalingam, Emma L. Reuschel, Mansi Purwar et autres

(SARS-CoV-2) has caused a global pandemic of COVID-19, resulting in cases of mild to severe respiratory distress and significant mortality. The global outbreak of this novel coronavirus has now infected >20 million people worldwide, with >5 million cases in the United States …

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Poster: Interaction of Water with Hydrophobic and Hydrophilic Surfaces

Graham Barry Bartlett Scott, Ian Kowalok, Michaela Helble, Scott J. Lehman et autres