Taylor Chen

Preclinical safety and bleeding evaluation in swine for a small interfering RNA-lipid nanoparticle that prevents excess fibrinogen synthesis

Monica S Seadler, Francesca Ferraresso, Halen Turner, Laura M. Ketelboeter et autres

us, ca (code pays fourni par la source)

AAVone: A cost-effective, single-plasmid solution for efficient AAV production with reduced DNA impurities

Rongze Yang, Ngoc Tam Tran, Taylor Chen, Mengtian Cui et autres

transduction efficiency, but a substantial reduction in DNA impurities from plasmid bacterial backbones and a marked reduction in non-functional snap-back genomes. The AAVone system does not pose a risk for generating replication-competent AAV contaminants. Furthermore, the AAVone system requires significantly less DNAs …

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AAVone: A Cost-Effective, Single-Plasmid Solution for Efficient AAV Production with Reduced DNA Impurities

Rongze Yang, Ngoc Tam Tran, Taylor Chen, Mengtian Cui et autres

ABSTRACT Currently, the most common approach for manufacturing GMP-grade adeno-associated virus (AAV) vectors involves transiently transfecting mammalian cells with three plasmids that carry the essential components for production. The requirement for all three plasmids to be transfected into a single cell and …

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siRNA-mediated reduction of a circulating protein in swine using lipid nanoparticles

Massimo F. Cau, Francesca Ferraresso, Monica S Seadler, Katherine E. Badior et autres

delivery of nucleic acids. Swine are a preferred animal model for studying the cardiovascular and immune systems, but there are limited strategies for genetic manipulation. Lipid nanoparticles (LNPs) efficiently deliver small interfering RNA (siRNA) to knock down circulating proteins, but swine are …

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The Rational Design of Highly Potent and Selective Covalent MAP2K7 Inhibitors

Karl A. Scheidt, Dalton Kim, Meghan Orr, Ada Kwong et autres

The mitogen-activated protein kinase signaling cascade is conserved across eukaryotes from yeast to humans, where it plays a central role regulating activities including proliferation, differentiation, and stress responses. This pathway propagates external stimuli through a series of phosphorylation events, allowing external signals …

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An Mb1-Cre-driven oncogenic Kras mutation results in a mouse model of T-acute lymphoblastic leukemia/lymphoma with short latency and high penetrance

Jacob J. Junco, Taylor Chen, Raushan Rashid, Maci Terrell et autres

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Concise Review: Regulation of Self-Renewal in Normal and Malignant Hematopoietic Stem Cells by Krüppel-Like Factor 4

Chun Shik Park, Andrew Henry Lewis, Taylor Chen, H. Daniel Lacorazza

Pluripotent and tissue-specific stem cells, such as blood-forming stem cells, are maintained through a balance of quiescence, self-renewal, and differentiation. Self-renewal is a specialized cell division that generates daughter cells with the same features as the parental stem cell. Although many factors …

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Abstract 145: Pharmacologic inhibition of SIAH2 stabilizes DYRK2 and inhibits survival and self-renewal in chronic myeloid leukemia (CML) leukemic stem cells

Chun Shik Park, Ye Shen, Andrew Henry Lewis, Koramit Suppipat et autres

Abstract Leukemia stem cells (LSCs) are a rare population able to sustain and recapitulate leukemia through a poorly understood mechanism of self-renewal. Because more than half of patients relapse after the cessation of TKI therapy, it is clear that a cure is …

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Rational development and characterization of humanized anti–EGFR variant III chimeric antigen receptor T cells for glioblastoma

Laura A. Johnson, John Scholler, Takayuki Ohkuri, Akemi Kosaka et autres

Chimeric antigen receptors (CARs) are synthetic molecules designed to redirect T cells to specific antigens. CAR-modified T cells can mediate long-term durable remissions in B cell malignancies, but expanding this platform to solid tumors requires the discovery of surface targets with limited …

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Validation of a humanized anti-EGFR variant III chimeric antigen receptor for a Phase I trial of CART-EGFRvIII in glioblastoma

Jennifer L. Brogdon, Andreas Loew, Pramod Thekkat, Neeraja Idamakanti et autres

Chimeric antigen receptors (CARs) are synthetic molecules designed to re-direct T cells to specific antigens; CAR-modified T cells can mediate long-term durable remissions in B cell malignancies, but expanding this platform to solid tumors requires the discovery of novel surface targets with …

Novel Chimeric Antigen Receptor T Cells for the Treatment of Hodgkin Lymphoma

Marco Ruella, Saad S. Kenderian, Olga Shestova, Taylor Chen et autres

Abstract Hodgkin lymphoma (HL) generally carries a good prognosis. However, 10-15% of patients relapse or are refractory to first-line therapy. These patients have a poor prognosis and would benefit from innovative approaches. Our group and others have demonstrated the clinical efficacy of …

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Abstract 4493: Rapamycin enhanced in vitro and in vivo activity of dexamethasone against acute lymphoblastic leukemia and lymphoma preclincal models via inhibition of p70S6 kinase activity

Yongku Ryu, Chong Zhang, Taylor Chen, Min H. Kang

Abstract Background: Glucocorticoids are an important part of therapy for lymphoid malignancies and understanding resistance mechanisms may enable designing drug combinations that will improve treatment outcome. We reported that rapamycin (RM) can enhance cytotoxicity of dexamethasone (DM) in cell culture models of …

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