Madison Conte

Lymphogranuloma Venereum Detection in Chlamydia trachomatis Positive Self-Collected Mail-in Male Rectal Samples in Maryland, United States

Justin P. Hardick, Madison Conte, Nisha Ramdeep, Yukari Carol Manabe et autres

BACKGROUND: Infection with Chlamydia trachomatis (CT) can have distinct clinical presentations, such as trachoma, or lymphogranuloma venereum (LGV). Certain populations are at greater risk for LGV acquisition and transmission, which may require a longer duration of therapy than other urogenital CT sexually …

us (code pays fourni par la source)

Characterizing SARS-CoV-2 Transcription of Subgenomic and Genomic RNAs During Early Human Infection Using Multiplexed Droplet Digital Polymerase Chain Reaction

Hyon S. Hwang, Che‐Min Lo, Michael J. Murphy, Tanner Grudda et autres

BACKGROUND: Control of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission requires understanding SARS-CoV-2 replication dynamics. METHODS: We developed a multiplexed droplet digital polymerase chain reaction (ddPCR) assay to quantify SARS-CoV-2 subgenomic RNAs (sgRNAs), which are only produced during active viral replication, …

us (code pays fourni par la source)

Mitigation of SARS-CoV-2 transmission at a large public university

Diana Rose E. Rañoa, Robin L. Holland, Fadi G. Alnaji, Kelsie J. Green et autres

In Fall 2020, universities saw extensive transmission of SARS-CoV-2 among their populations, threatening health of the university and surrounding communities, and viability of in-person instruction. Here we report a case study at the University of Illinois at Urbana-Champaign, where a multimodal "SHIELD: …

us (code pays fourni par la source)

Daily longitudinal sampling of SARS-CoV-2 infection reveals substantial heterogeneity in infectiousness

Ruian Ke, Pamela P. Martinez, Rebecca L. Smith, Laura L. Gibson et autres

us (code pays fourni par la source)

Longitudinal Analysis of SARS-CoV-2 Vaccine Breakthrough Infections Reveals Limited Infectious Virus Shedding and Restricted Tissue Distribution

Ruian Ke, Pamela P. Martinez, Rebecca L. Smith, Laura L. Gibson et autres

Background: The global effort to vaccinate people against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during an ongoing pandemic has raised questions about how vaccine breakthrough infections compare with infections in immunologically naive individuals and the potential for vaccinated individuals to transmit …

us (code pays fourni par la source)

Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

Roger S. Zou, Momčilo M. Gavrilov, Yang Liu, Dominique Rasoloson et autres

Nucleic acid detection is essential for numerous biomedical applications, but often requires complex protocols and/or suffers false-positive readouts. Here, we describe SENTINEL, an approach that combines isothermal amplification with a sequence-specific degradation method to detect nucleic acids with high sensitivity and sequence-specificity. …

us (code pays fourni par la source)

Characterizing SARS-CoV-2 transcription of subgenomic and genomic RNAs during early human infection using multiplexed ddPCR

Hyon S. Hwang, Che‐Min Lo, Michael J. Murphy, Tanner Grudda et autres

Abstract Control of SARS-CoV-2 (SCV-2) transmission is a major priority that requires understanding SCV-2 replication dynamics. We developed and validated novel droplet digital PCR (ddPCR) assays to quantify SCV-2 subgenomic RNAs (sgRNAs), which are only produced during active viral replication, and discriminate …

us (code pays fourni par la source)

Longitudinal analysis of SARS-CoV-2 vaccine breakthrough infections reveal limited infectious virus shedding and restricted tissue distribution

Ruian Ke, Pamela P. Martinez, Rebecca L. Smith, Laura L. Gibson et autres

The global effort to vaccinate people against SARS-CoV-2 in the midst of an ongoing pandemic has raised questions about the nature of vaccine breakthrough infections and the potential for vaccinated individuals to transmit the virus. These questions have become even more urgent …

us (code pays fourni par la source)

Mitigation of SARS-CoV-2 Transmission at a Large Public University

Diana Rose E. Rañoa, Robin L. Holland, Fadi G. Alnaji, Kelsie J. Green et autres

In the Fall of 2020, many universities saw extensive transmission of SARS-CoV-2 among their populations, threatening the health of students, faculty and staff, the viability of in-person instruction, and the health of surrounding communities. 1, 2 Here we report that a multimodal …

us (code pays fourni par la source)

Daily sampling of early SARS-CoV-2 infection reveals substantial heterogeneity in infectiousness

Ruian Ke, Pamela P. Martinez, Rebecca L. Smith, Laura L. Gibson et autres

The dynamics of SARS-CoV-2 replication and shedding in humans remain poorly understood. We captured the dynamics of infectious virus and viral RNA shedding during acute infection through daily longitudinal sampling of 60 individuals for up to 14 days. By fitting mechanistic models, …

us (code pays fourni par la source)

Longitudinal Assessment of Diagnostic Test Performance Over the Course of Acute SARS-CoV-2 Infection

Rebecca L. Smith, Laura L. Gibson, Pamela P. Martinez, Ruian Ke et autres

BACKGROUND: Serial screening is critical for restricting spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by facilitating timely identification of infected individuals to interrupt transmission. Variation in sensitivity of different diagnostic tests at different stages of infection has not been well …

us (code pays fourni par la source)

Longitudinal assessment of diagnostic test performance over the course of acute SARS-CoV-2 infection

Rebecca L. Smith, Laura L. Gibson, Pamela P. Martinez, Ruian Ke et autres

WHAT IS ALREADY KNOWN ABOUT THIS TOPIC?: Diagnostic tests and sample types for SARS-CoV-2 vary in sensitivity across the infection period. WHAT IS ADDED BY THIS REPORT?: We show that both RTqPCR (from nasal swab and saliva) and the Quidel SARS Sofia …

us (code pays fourni par la source)