Jordi Soriano

Limited Detectability of Network Functional Alterations in a Tauopathy Model Using Mouse Primary Cortical Cultures

Clara F. López-León, Julia Sala‐Jarque, José Antonio del Rı́o, Jordi Soriano

Background: Tauopathies are neurodegenerative disorders characterized by the abnormal hyperphosphorylation and aggregation of the microtubule-associated protein tau, leading to disrupted neuronal connectivity and progressive brain dysfunction. Despite their clinical relevance, most in vitro models have focused primarily on molecular and cellular aspects, …

es, us (code pays fourni par la source)

Reservoir computing in simulated neuronal cultures: Effect of network structure

Akke Mats Houben, Anna-Christina Haeb, J Garcia-Ojalvo, Jordi Soriano

Biological neurons are emerging as attractive candidates for artificial intelligence and machine learning applications given their natural energy efficiency and self-repair capacity. However, they differ from their idealized artificial counterparts. Biological neurons have highly variable and noisy dynamics and display intrinsic spontaneous …

es (code pays fourni par la source)

Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures

Anna-Christina Haeb, Hideaki Yamamoto, Paul Roach, Daniel J. Merryweather et autres

Complementary metal-oxide-semiconductor (CMOS)-based high-density microelectrode arrays (HD-MEAs) enable neuronal recordings with high spatiotemporal resolution. However, integrating polydimethylsiloxane (PDMS) microstructures onto HD-MEA surfaces to control network architecture is currently challenging and platform specific. Here, we present a protocol for PDMS fabrication, HD-MEA chip …

es, jp, gb (code pays fourni par la source)

Experiments on a Tauopathy Model with Mice Primary Cortical Cultures: the Difficulty of Observing Network Functional Alterations

Clara F. López-León, Julia Sala‐Jarque, José Antonio del Rı́o, Jordi Soriano

Tauopathies are a group of neurodegenerative diseases associated to a pathological, hyperphosphorylated state of the microtubule-associated protein tau which, in the brain, cause a progressive degradation of neuronal connections that lead to overt network-wide functional alterations. Despite the importance of these diseases, …

Role of connectivity anisotropies in the dynamics of cultured neuronal networks

Akke Mats Houben, Jordi García‐Ojalvo, Jordi Soriano

An inherent challenge in designing laboratory-grown, engineered living neuronal networks lies in predicting the dynamic repertoire of the resulting network and its sensitivity to experimental variables. To fill this gap, and inspired by recent experimental studies, we present a numerical model designed …

es (code pays fourni par la source)

Variability vs. phenotype: Multimodal analysis of Dravet syndrome brain organoids powered by deep learning

Isabel Turpin-Moreno, Adriana Modrego, Andrea Martí-Sarrias, Laura García‐González et autres

gene. Brain organoids (BOs) have emerged as reliable models for neurodevelopmental genetic disorders, reproducing human brain developmental milestones and rising as a promising drug testing tool. Here, we determined the underlying molecular DS pathophysiology affecting neuronal connectivity, revealing an early onset excitatory-inhibitory …

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Protocol to generate PDMS topographical patterns for hiPSC-derived or rat primary neuronal cultures

Anna-Christina Haeb, Mireia Olives-Verger, Jordi Soriano

Soft lithography is a promising technique to fabricate tailored substrates on polydimethylsiloxane (PDMS) casts. Here, we present a protocol to generate PDMS topographical patterns for neuronal networks in vitro . The protocol outlines the development of a two-level resin master mold with …

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In-silico study on the dynamical and topological relationships between three-dimensional cultures and relative slices

Leonardo Della Mea, Angelo Piga, Monica Frega, Jordi Soriano

Three-dimensional (3D) in-vitro neuronal cultures represent a pioneering technological advance in exploring dynamics, function and dysfunction in environments that mimic brain's rich spatial organization. However, the recording of activity on the entire 3D network remains a challenge as researchers often resort to …

it, es (code pays fourni par la source)

Modular architecture confers robustness to damage and facilitates recovery in spiking neural networks modeling in vitro neurons

Takuma Sumi, Akke Mats Houben, Hideaki Yamamoto, Hideyuki Kato et autres

Impaired brain function is restored following injury through dynamic processes that involve synaptic plasticity. This restoration is supported by the brain’s inherent modular organization, which promotes functional separation and redundancy. However, it remains unclear how modular structure interacts with synaptic plasticity to …

jp, es (code pays fourni par la source)

Modular architecture confers robustness to damage and facilitates recovery in spiking neural networks modeling in vitro neurons

Takuma Sumi, Akke Mats Houben, Hideaki Yamamoto, Hideyuki Kato et autres

Abstract Impaired brain function is restored following injury through dynamic processes that involve synaptic plasticity. This restoration is supported by the brain’s inherent modular organization, which promotes functional separation and redundancy. However, it remains unclear how the modular structure interacts with synaptic …

jp, es (code pays fourni par la source)

Variability vs Phenotype: multimodal analysis of Dravet Syndrome Brain Organoids powered by Deep Learning

Isabel Turpin-Moreno, Adriana Modrego, Andrea Martí-Sarrias, Anna Christina Haeb et autres

ABSTRACT Brain organoids (BO) have risen as a reliable model for neurodelopmental disorders (ND), reproducing human brain development milestones. However, their significant intra- and inter-organoid variability compromises their use in advanced tasks such as drug testing. Overcoming experimental variability is crucial for …

es (code pays fourni par la source)

Integration of Calcium Imaging Traces via Deep Generative Modeling

Josep Maria Canals, Jordi Soriano, Jordi Abante

Calcium imaging allows for the parallel measurement of large neuronal populations in a spatially resolved and minimally invasive manner, and has become a gold-standard for neuronal functionality. While deep generative models have been successfully applied to study the activity of neuronal ensembles, …