Monica Frega

Astrocytes contribute to olanzapine-mediated reversal of kleefstra syndrome-associated neurodevelopmental regression

Karlijn Vermeulen‐Kalk, Shan Wang, Joost Kummeling, Britt Mossink et autres

Kleefstra syndrome (KLEFS1) results from EHMT1 haploinsufficiency and is characterized by variable neurodevelopmental delays and psychopathology. Developmental regression, marked by the sudden loss of previously acquired daily life skills during late puberty or early adulthood, has emerged as a severe complication in …

nl, us (code pays fourni par la source)

Distinct effects of different metabolic stress models on human-derived neuronal networks

Linda Collo, Eva J.H.F. Voogd, Giulia Parodi, Marloes Levers et autres

Abstract Different in vitro models are widely used as experimental platforms to assess neuronal responses to metabolic stress and test potential treatments for patients with ischemic stroke. Results of those studies depend on the stress models used, and the link between cell …

it, nl (code pays fourni par la source)

Burst suppression: a default brain state associated with loss of network complexity

Nina Doorn, Gerco C. Hassink, Monica Frega, Michel J. A. M. van Putten

Burst suppression (BS) is a highly stereotyped EEG pattern observed across a wide range of clinical contexts, from general anesthesia and postanoxic coma to neonatal encephalopathy. Despite its consistent appearance, BS comprises two distinct forms with markedly different implications. BS with identical …

nl, it (code pays fourni par la source)

Electrophysiological Alteration in Duplication 15q hiPSCs-Derived in Vitro Model

Linda Collo, Verdiana Pullano, Giulia Parodi, Francesca Scilef et autres

it, nl (code pays fourni par la source)

Does sex matter in neurons’ response to hypoxic stress?

Eva J.H.F. Voogd, Marloes Levers, Jeannette Hofmeijer, Monica Frega et autres

BACKGROUND: Stroke exhibits significant sex differences in incidence, response to treatment, and outcome. Preclinical studies suggest that hormones, particularly estrogens, are key to differential sensitivity, as female neurons demonstrate enhanced resilience compared to males in both in vivo and in vitro models. …

nl (code pays fourni par la source)

In-silico study on the dynamical and topological relationships between three-dimensional cultures and relative slices

Leonardo Della Mea, Angelo Piga, Monica Frega, Jordi Soriano

Three-dimensional (3D) in-vitro neuronal cultures represent a pioneering technological advance in exploring dynamics, function and dysfunction in environments that mimic brain's rich spatial organization. However, the recording of activity on the entire 3D network remains a challenge as researchers often resort to …

it, es (code pays fourni par la source)

HiPSC-Derived Neuronal Networks on Micro-Electrode Arrays: a Functional Model of the Ischemic Penumbra

Linda Collo, Giulia Parodi, Giorgia Zanini, Eva J.H.F. Voogd-Sikkens et autres

Acute ischemic stroke, caused by cerebral blood flow blockage, leads to neuronal damage driven by a complex pathophysiological cascade of processes, among which excessive glutamate release plays an important role. In this study, we developed a human-derived in vitro model mimicking the …

it, nl (code pays fourni par la source)

Automated inference of disease mechanisms in patient-hiPSC-derived neuronal networks

Nina Doorn, Michel J. A. M. van Putten, Monica Frega

Human induced pluripotent stem cells (hiPSCs)-derived neuronal networks on multi-electrode arrays (MEAs) are a powerful tool for studying neurological disorders. The electric activity patterns of these networks differ between healthy and patient-derived neurons, reflecting underlying pathology. However, elucidating these underlying molecular mechanisms …

nl, it (code pays fourni par la source)

Hypothermia improves neuronal network recovery in a human-derived in vitro model of oxygen-deprivation

Eva J.H.F. Voogd, Marloes Thijs, Marloes Levers, Jeannette Hofmeijer et autres

Mild therapeutic hypothermia showed potential neuroprotective properties during and after cerebral hypoxia or ischemia in experimental animal studies. However, in clinical trials, where hypothermia is mainly applied after reperfusion, results were divergent and neurophysiological effects unclear. In our current study, we employed …

nl (code pays fourni par la source)

In vitro electrophysiological drug testing on neuronal networks derived from human induced pluripotent stem cells

Giulia Parodi, Giorgia Zanini, Linda Collo, Roberta Impollonia et autres

In vitro models for drug testing constitute a valuable and simplified in-vivo-like assay to better comprehend the biological drugs effect. In particular, the combination of neuronal cultures with Micro-Electrode Arrays (MEAs) constitutes a reliable system to investigate the effect of drugs aimed …

it, nl (code pays fourni par la source)

CACNA1A haploinsufficiency leads to reduced synaptic function and increased intrinsic excitability

Marina P. Hommersom, Nina Doorn, Sofía Puvogel, Elly Lewerissa et autres

Haploinsufficiency of CACNA1A, encoding the pore-forming α1 subunit of P/Q-type voltage-gated calcium channels, is associated with a clinically variable phenotype ranging from cerebellar ataxia to neurodevelopmental syndromes with epilepsy and intellectual disability. To understand the pathological mechanisms of CACNA1A loss-of-function variants, we …

nl (code pays fourni par la source)

Does sex matter in neurons’ response to hypoxic stress?

Eva J.H.F. Voogd, Marloes Levers, Jeannette Hofmeijer, Monica Frega et autres

Abstract Background Stroke exhibits significant sex differences in incidence, response to treatment and outcome. Preclinical studies suggest that hormones, particularly estrogens, are key to differential sensitivity, as female neurons demonstrate enhanced resilience compared to males in both in vivo and in vitro …

nl (code pays fourni par la source)