Abrahan Hernández‐Hernández

Comparative analysis of whole genome amplification kits for single-cell genome analysis

Abrahan Hernández‐Hernández, Pontus Höjer, Michelle Ljungmark, Anastasios Glaros et autres

ABSTRACT Whole genome amplification (WGA) enables detailed analysis of genomic heterogeneity at the cellular level. Here, we systematically compare two commercially available WGA technologies on single cells; Multiple Displacement Amplification in the Qiagen REPLI-g kit (MDA/REPLI-g) and Primary Template-directed Amplification in the …

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Identification of potential new T cell activation molecules: a Bioinformatic Approach

Mario Morales-Martínez, David Andón-García, Karla Aimee Patiño-Santiago, Jesús Miguel Parga-Ortega et autres

T-cell activation is central for the initiation of T cell mediated adaptive immune response and is the result of the close communication between the Antigen Presenting Cell (APC) and the T lymphocyte. Although T-cell activation is currently well understood, and many intracellular …

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Regulation of meiotic telomere dynamics through membrane fluidity promoted by AdipoR2-ELOVL2

Jingjing Zhang, Mario Ruiz, Per‐Olof Bergh, Marcus Henricsson et autres

The cellular membrane in male meiotic germ cells contains a unique class of phospholipids and sphingolipids that is required for male reproduction. Here, we show that a conserved membrane fluidity sensor, AdipoR2, regulates the meiosis-specific lipidome in mouse testes by promoting the …

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Spermiogenesis alterations in the absence of CTCF revealed by single cell RNA sequencing

Ulises Torres-Flores, Fernanda Díaz-Espinosa, Tayde López-Santaella, Rosa Rebollar‐Vega et autres

CTCF is an architectonic protein that organizes the genome inside the nucleus in almost all eukaryotic cells. There is evidence that CTCF plays a critical role during spermatogenesis as its depletion produces abnormal sperm and infertility. However, defects produced by its depletion …

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The Transcriptomic Landscape of Pediatric Astrocytoma

Abrahan Hernández‐Hernández, Tayde López-Santaella, Aranxa Torres-Caballero, Amarantha Serrato et autres

Central nervous system tumors are the most common solid neoplasia during childhood and represent one of the leading causes of cancer-related mortality. Tumors arising from astrocytic cells (astrocytomas) are the most frequently diagnosed, and according to their histological and pathological characteristics, they …

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A cryo-fixation protocol to study the structure of the synaptonemal complex

Rosario Ortíz, Olga M. Echeverría, Sergej Masich, Christer Höög et autres

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Epigenetic Dysregulation of Mammalian Male Meiosis Caused by Interference of Recombination and Synapsis

Roberto de la Fuente, Florencia Pratto, Abrahan Hernández‐Hernández, Marcia Manterola et autres

Meiosis involves a series of specific chromosome events, namely homologous synapsis, recombination, and segregation. Disruption of either recombination or synapsis in mammals results in the interruption of meiosis progression during the first meiotic prophase. This is usually accompanied by a defective transcriptional …

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Extracellular Vesicles from Human Plasma Show a Distinctive Proteome and miRNome Profile in Patients with Severe Cutaneous Adverse Reactions

Orlando Salinas-Jaramillo, Alejandra Monroy-Arreola, Juan Sebastian Herrera-Noreña, Ana Laura Guzmán-Ortiz et autres

Cutaneous drug-induced reactions are immune-mediated responses that can lead to life-threatening diseases such as drug reaction with eosinophilia and systemic symptoms (DRESS), Stevens–Johnson syndrome, and toxic epidermal necrolysis, collectively known as severe cutaneous adverse reactions (SCARs). Unfortunately, they cannot be predicted during …

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A close-to-native structure of the synaptonemal complex

Rosario Ortíz, Olga M. Echeverría, Sergej Masich, Christer Höög et autres

Abstract Genetic variability in sexually reproducing organisms results from an exchange of genetic material between homologous chromosomes. The genetic exchange mechanism is dependent on the synaptonemal complex (SC), a protein structure localized between the homologous chromosomes. Current structural models of the SC …

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Spermiogenesis alterations in the absence of CTCF revealed by single cell RNA sequencing

Ulises Torres-Flores, Fernanda Díaz-Espinosa, Tayde López-Santaella, Rosa Rebollar‐Vega et autres

Abstract CTCF is an architectonical protein that organizes the genome inside the cell nucleus in almost all eukaryotic cells. There is evidence that CTCF plays a critical role during spermatogenesis as its depletion produces abnormal sperm and infertility. However, the defects produced …

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Benzo[ghi]perylene Induces Morphological and Genotoxic Effects in Human Bronchial Cells Independently of The AhR Pathway

Zelmy Castro-Gálvez, Montserrat Zaragoza‐Ojeda, Abrahan Hernández‐Hernández, Francisco Arenas‐Huertero

Abstract Polycyclic aromatic hydrocarbons (PAH) are organic compounds found in the contaminated atmosphere of Mexico City, where the PAH present with highest concentration is benzo[ghi]perylene [B[ghi]p]. We recently demonstrated that double-stranded breaks in DNA appear after 3h of exposure, whereas cellular changes …

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Synaptonemal complex formation produces a particular arrangement of the lateral element-associated DNA

Rosario Ortíz, Silvia Juárez Chavero, Olga M. Echeverría, Abrahan Hernández‐Hernández

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