Shunji Li

Deep learning-based microfluidic rapid phenotypic AST uncovers early resistance trajectories

Shunji Li, Chenxi Dai, Dongjuan Chen, Chao Wan et autres

cn (code pays fourni par la source)

A Tonsil Organoid Model for Epstein-Barr Virus-Host Immune Interactions in Germinal Centers 2308488

Yizhe Sun, Mahina Mitul, Shunji Li, Ling Zhong et autres

Abstract Introduction Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that establishes lifelong persistence in B lymphocytes by adopting distinct latency programs within lymphoid tissues such as the tonsils. In vitro, EBV infection of primary B cells drives transformation into latency III …

us (code pays fourni par la source)

Epstein-Barr virus transformation creates a methionine-dependent ferroptosis vulnerability in B cells

Shaowen White, Rui Guo, Bidisha Mitra, H Li et autres

SUMMARY Epstein-Barr virus (EBV) causes over 200,000 cancers annually, including immunoblastic lymphomas in immunosuppressed hosts. Most transformed cells arrest, yet survive when deprived of the essential amino acid methionine. We instead find that EBV transformed lymphoblastoid cell lines (LCLs), which model the …

us (code pays fourni par la source)

Arginine metabolism supports de novo pyrimidine biosynthesis to block DNA damage and maintain Epstein-Barr virus latency

Shaowen White, Yifei Liao, Yue Wang, Shunji Li et autres

ABSTRACT Incompletely understood mechanisms serve to maintain Epstein-Barr virus (EBV) latency, in which viral oncogene(s) are expressed, but lytic antigens are not expressed. Shortly after the discovery of EBV and even before it was named, Werne and Gertrude Henle identified that restriction …

us, il (code pays fourni par la source)

The HUSH Complex Dictates EBV-transformed B cell Sensitivity to NK Cell Surveillance Through Repression of NKG2A Ligand γ-Proto-cadherin

Yizhe Sun, Isabella Y. Kong, Wanyu Li, Jesse S. Plung et autres

Summary Natural Killer (NK) cells control Epstein-Barr virus (EBV), though how EBV+ B-cells escape NK surveillance to form tumors remains unknown. To gain insights, we performed a human genome-wide CRISPR-Cas9 screen in EBV-transformed lymphoblastoid cell lines (LCL). This revealed that the HUSH …

us, es (code pays fourni par la source)

A CRISPR-Cas9 screen Reveals STEEP1 as a Key Host Dependency Factor for Epstein-Barr Virus Latent Membrane Protein 1 Trafficking and Signaling

Shunji Li, Yizhe Sun, Yifei Liao, Ling Zhong et autres

Abstract The Epstein-Barr virus (EBV) oncogene Latent membrane protein 1 (LMP1) is essential for B-cell transformation into continuously growing lymphoblastoid cell lines. LMP1 traffics to plasma membrane and intracellular signaling sites to mimic aspects of signaling by the B cell co-receptor CD40. …

us (code pays fourni par la source)

Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings

Chao Wan, Huijuan Yuan, Chenxi Dai, Dongjuan Chen et autres

The misuse of antibiotics has accelerated the rise of antimicrobial resistance (AMR), particularly in resource-limited regions, posing a critical threat to global public health. Current antibiotic susceptibility test (AST) technologies are trapped in a paradox: regions most in need of rapid diagnostics …

cn, ro (code pays fourni par la source)

Epstein-barr virus latent membrane protein 1 targets cIAP1, cIAP2 and TRAF2 for proteasomal degradation to activate the non-canonical NF-κB pathway

Yizhe Sun, Shunji Li, Bidisha Mitra, Ling Zhong et autres

The Epstein-Barr virus (EBV) oncoprotein Latent Membrane Protein 1 (LMP1) is expressed in multiple malignancies and is critical for B-cell immortalization. LMP1 constitutively activates NF-κB signaling pathways, which are essential for EBV-mediated B cell transformation and for transformed B cell survival. Reverse …

us (code pays fourni par la source)

Self-Powered Gravity Domino Microfluidics for Rapid At-Home Immunoassays with Artificial Intelligence

Huijuan Yuan, Chao Wan, Xiang Liu, Chenxi Dai et autres

At-home testing facilitates early screening and longitudinal monitoring of immune disorders, significantly enhancing patient engagement in personalized healthcare management. This convenient self-testing capability primarily stems from advancements in microfluidic technology, with the microfluidic chain reaction (MCR) demonstrating particular efficacy. MCR platforms incorporate …

cn (code pays fourni par la source)

AI-powered programmable wetting-delamination μPAD for point-of-care food safety detection

Chenxi Dai, Hao Huang, Yunhao Zhang, Yingying Liu et autres

cn (code pays fourni par la source)

Epstein-Barr Virus Latent Membrane Protein 1 targets cIAP1, cIAP2 and TRAF2 for Proteasomal Degradation to Activate the Non-canonical NF-κB Pathway

Yizhe Sun, Shunji Li, Bidisha Mitra, Ling Zhong et autres

The Epstein-Barr virus oncoprotein Latent Membrane Protein 1 (LMP1) is expressed in multiple malignancies and is critical for B-cell immortalization. LMP1 constitutively activates NF-κB signaling pathways, which are essential for EBV-mediated B cell transformation and for transformed B cell survival. Reverse genetic …

us (code pays fourni par la source)

Deep Learning-Enhanced Hand-Driven Spatial Encoding Microfluidics for Multiplexed Molecular Testing at Home

Ying Zhang, Dongjuan Chen, Xin Tang, Tao Xu et autres

The frequent global outbreaks of viral infectious diseases have significantly heightened the urgent demand for molecular testing at home. However, the labor-intensive sample preparation and nucleic acid amplification steps, along with the complexity and bulkiness of detection equipment, have limited the large-scale …

cn, mx (code pays fourni par la source)