Maxime Denis

New High-Throughput Method for Aluminum Content Determination in Vaccine Formulations

Lorenzo Di Meola, Daniela Pasqui, Chiara Tigli, Stephen L. J. Luckham et autres

OBJECTIVE: This manuscript describes an innovative, non-destructive, high-throughput method for the quantification of aluminum hydroxide in aluminum-adjuvanted vaccines, eliminating the need of reagents and providing real-time results. The method is based on a spectrophotometric principle, and several model proteins were studied and …

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Straight to the point: targeted mRNA-delivery to immune cells for improved vaccine design

Bruna Clemente, Maxime Denis, Camila Pedroso Silveira, Francesca Schiavetti et autres

With the deepening of our understanding of adaptive immunity at the cellular and molecular level, targeting antigens directly to immune cells has proven to be a successful strategy to develop innovative and potent vaccines. Indeed, it offers the potential to increase vaccine …

Evolution of Vaccines Formulation to Tackle the Challenge of Anti-Microbial Resistant Pathogens

Francesco Tognetti, Massimiliano Biagini, Maxime Denis, Francesco Berti et autres

The increasing diffusion of antimicrobial resistance (AMR) across more and more bacterial species emphasizes the urgency of identifying innovative treatment strategies to counter its diffusion. Pathogen infection prevention is among the most effective strategies to prevent the spread of both disease and …

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The dual behaviour of β-vinylporphyrins in the presence of α,α′-dioxothiones

Cristina J. Dias, Francesco Papi, Maxime Denis, Cristina Nativi et autres

α,α′-Dioxothione react with meso -tetraarylporphyrins bearing vinyl functionalities as a heterodienophiles or as a heterodienes. The dual behaviour is dependent on the absence or on the presence of a metal ion on the porphyrin inner core.

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Identification of the Allosteric Binding Site for Thiazolopyrimidine on the C-Type Lectin Langerin

Hengxi Zhang, Carlos Pablo Modenutti, Yelha Phani Kumar Nekkanti, Maxime Denis et autres

Langerin is a mammalian C-type lectin expressed on Langerhans cells in the skin. As an innate immune cell receptor, Langerin is involved in coordinating innate and adaptive immune responses against various incoming threats. We have previously reported a series of thiazolopyrimidines as …

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Identification of the Allosteric Binding Site for Thiazolopyrimidine on the C-Type Lectin Langerin

Hengxi Zhang, Carlos Pablo Modenutti, Yelha Phani Kumar Nekkanti, Maxime Denis et autres

Langerin is a mammalian C-type lectin expressed on Langerhans cells in the skin. As an innate immune cell receptor, Langerin is involved in coordinating innate and adaptive immune responses against various incoming threats. We have previously reported a series of thiazolopyrimidines as …

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Targeting undruggable carbohydrate recognition sites through focused fragment library design

Elena Shanina, Sakonwan Kuhaudomlarp, Eike Siebs, Felix F. Fuchsberger et autres

Abstract Carbohydrate-protein interactions are key for cell-cell and host-pathogen recognition and thus, emerged as viable therapeutic targets. However, their hydrophilic nature poses major limitations to the conventional development of drug-like inhibitors. To address this shortcoming, four fragment libraries were screened to identify …

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Drug-like Inhibitors of DC-SIGN Based on a Quinolone Scaffold

Hengxi Zhang, Ondřej Daněk, Dmytro Makarov, Stanislav Rádl et autres

DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin) is a pattern recognition receptor expressed on immune cells and involved in the recognition of carbohydrate signatures present on various pathogens, including HIV, Ebola, and SARS-CoV-2. Therefore, developing inhibitors blocking the carbohydrate-binding site of DC-SIGN …

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Fucosylated Ubiquitin and Orthogonally Glycosylated Mutant A28C: Conceptually New Ligands for Burkholderia Ambifaria Lectin (BambL)†

Cristina Nativi, Sakonwan Kuhaudomlarp, Linda Cerofolini, Sabrina Santarsia et autres

Ubiquitin as scaffold protein and aryl-α-O-fucoside as determinant to achieve conceptually new ligands with high affinity for Burkholderia ambifaria lectin are described. Ub mutant A28C which displays a Cys residue in addition to the eight Lys residues was expressed. The resulting scaffold …

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Cover Feature: The Photocatalyzed Thiol‐ene reaction: A New Tag to Yield Fast, Selective and reversible Paramagnetic Tagging of Proteins (ChemPhysChem 9/2020)

Maxime Denis, Charlotte A. Softley, Stefano Giuntini, Matteo Gentili et autres

The Cover Feature illustrates the photo-initialization by UV light of the thiol-ene reaction between the alkene of a newly designed paramagnetic tag and the thiol group of the cysteine residue on a model protein. The protein tagging occurs in 1 hour. We …

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The Photocatalyzed Thiol‐ene reaction: A New Tag to Yield Fast, Selective and reversible Paramagnetic Tagging of Proteins

Maxime Denis, Charlotte A. Softley, Stefano Giuntini, Matteo Gentili et autres

Paramagnetic restraints have been used in biomolecular NMR for the last three decades to elucidate and refine biomolecular structures, but also to characterize protein-ligand interactions. A common technique to generate such restraints in proteins, which do not naturally contain a (paramagnetic) metal, …

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Design, Synthesis and Optimization of Paramagnetic Tags for NMR Spectroscopy

Maxime Denis

Paramagnetic restraints have been used in biomolecular NMR for the last three decades in order to elucidate and refine structures but also to characterize protein/ligand interactions. Pseudo-Contact Shifts (PCS) are the most commonly used restraints, consisting in measuring a shift between a …