Karen A. Proudfoot

Purification of the T-Cell Receptor ζ-Chain: Covalent Modification by 4-(2-Aminoethyl)- benzenesulfonyl Fluoride

Beatrice Marcy Sweeney, Karen A. Proudfoot, Angela Parton, Linda M. King et autres

Human p59fyn(T) regulates OKT3-induced calcium influx by a mechanism distinct from PIP2 hydrolysis in Jurkat T cells.

Kevin P. Rigley, Patrick M. Slocombe, Karen A. Proudfoot, S Wahid et autres

The earliest biochemical event after cross-linking of TCR is the tyrosine phosphorylation of a variety of substrates. At least three nonreceptor tyrosine kinases have been implicated in this signaling cascade: p59fyn(T), p56lck, and ZAP-70. Recently, PLC gamma 1 has been shown to …

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Improved tumor targeting with chemically cross-linked recombinant antibody fragments.

DJ King, Alison V. Turner, APH Farnsworth, Adair et autres

The construction and use of recombinant chimeric and later fully humanized (CDR-grafted) antibodies to tumor-associated antigens has reduced the immune response generated to these antibodies in clinical studies. However, their long circulating half-life is a disadvantage for tumor imaging and therapy. Fragments …

Expression, purification and characterization of B72.3 Fv fragments

David J. King, Olwyn Byron, A. Mountain, Neil Weir et autres

The Fv fragment of the antibody B72.3 has been produced by expression in both a mammalian and microbial system, namely Chinese hamster ovary (CHO) cells and Escherichia coli. In both cases secretion of the Fv into the culture medium was achieved, with …

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Purification of recombinant chimeric B72.3 Fab′ and F(ab′)2 using streptococcal protein G

Karen A. Proudfoot, Christopher J. Torrance, Alastair D.G. Lawson, David J. King

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Expression, purification and characterization of a mouse-human chimeric antibody and chimeric Fab' fragment

David J. King, John R. Adair, Sarojani Angal, Dominic Löw et autres

B72.3 is a mouse monoclonal antibody against a tumour-associated antigen, TAG72, which recognizes breast, ovarian and colorectal tumour tissue. A mouse-human chimeric version of B72.3 has been expressed in Chinese-hamster ovary cells. This molecule has the binding specificity of B72.3 and constant …

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Purification of a hybrid plasminogen activator protein

Janet Deistung, Deidree M. Forde, James P. O’Connell, Karen A. Proudfoot et autres

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Studies on the hyperplasia (‘regeneration’) of the rat liver following partial hepatectomy. Changes in lipid peroxidation and general biochemical aspects

T. F. Slater, Kevin H. Cheeseman, Chiara Benedetto, Margaret M. Collins et autres

Using the experimental model of partial hepatectomy in the rat, we have examined the relationship between cell division and lipid peroxidation activity. In rats entrained to a regime of 12 h light/12 h dark and with a fixed 8 h feeding period …

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Lipid Peroxidation, Antioxidants and Regenerating Rat Liver

Kevin H. Cheeseman, Sean Emery, Susan P. Maddix, Karen A. Proudfoot et autres

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Free radical mechanisms in relation to tissue injury

T. F. Slater, Kevin H. Cheeseman, Michael J. Davies, Karen A. Proudfoot

The present paper is a broad-ranging account of free-radical biochemistry in general, and of free-radical mechanisms of tissue injury in particular.Because it is broad-ranging within tight constraints of length it is necessarily lacking in detail on some issues of relevance; the following …

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Lipid peroxidation in regenerating rat liver

Kevin H. Cheeseman, M. W. Collins, Susan P. Maddix, Anna Franca Milia et autres

Rats entrained to a strictly regulated lighting and feeding schedule have been subjected to partial hepatectomy or a sham operation. In the partially hepatectomised animals the period of liver regeneration is characterised by regular bursts of thymidine kinase activity. Liver microsomes from …

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Studies on lipid peroxidation in normal and tumour tissues. The Novikoff rat liver tumour

Kevin H. Cheeseman, M. W. Collins, Karen A. Proudfoot, T. F. Slater et autres

A study has been made of the factors that contribute to the decreased rates of lipid peroxidation under different pro-oxidant conditions in intact Novikoff tumour cells, and in microsomal suspensions prepared from Novikoff tumour cells, compared with isolated normal rat hepatocytes and …

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