Douglas B. Craig

Interplay between fibroblast growth factor 9 (FGF9) and CD44 underlies 46,XY disorders of sex development in Kruppel‐like factor 1 (KLF‐1)‐E325K‐associated congenital dyserythropoietic anaemia (CDA‐IV)

Yaddanapudi Ravindranath, Douglas B. Craig, Prahlad Parajuli, Shruti Bagla et autres

A heterozygous Kruppel-like factor 1 (KLF1) E325K mutation causes a severe form of congenital dyserythropoietic anaemia (CDA), designated CDA-IV (Online Mendelian Inheritence in Man, OMIM 613673), a rare disease with only 15 cases known. In addition to haemolytic anaemia, manifest often at …

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Proteogenomic profiling defines progression-associated biology in high-risk endometrial cancer

G. Larry Maxwell, Nicholas W. Bateman, Kathleen M. Darcy, Chunqiao Tian et autres

Patients with high-risk endometrial carcinoma (HR-EC) experience substantial mortality despite multimodality therapy, molecular classification and contemporary care. We determined whether proteomic tumor programs capture lethal risk beyond established clinical and genomic classifiers and may inform translational investigations. We performed integrated clinical, genomic, …

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Measurements on the Distribution of E. coli β-Galactosidase Electrophoretic Mobilities Suggest Heterogeneous Ligand Binding

Douglas B. Craig, Gurleen Aulakh, Charlize Yerex

The distribution of electrophoretic mobility of E. coli β-galactosidase was measured in the presence of citrate, α-ketoglutarate, oxaloacetate, and pyrophosphate and compared to that of the enzyme in the absence of ligand. In the presence of citrate the mobility was found to …

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Analysis of Serotonin in Caenorhabditis Elegans Subjected to Micro-Dosing with Psilocybin| Iris Publishers

Douglas B. Craig

To accelerate the growth of scientific learning through research gathered from all over the world. We want to be the catalysts for new discoveries in medicine, neurosurgery and other fields of science that will help cure diseases and find new ways of …

Advancing Scientific Workflows: A Human-LLM Note-Taking System with Case-Based Reasoning

Douglas B. Craig

Human-centered artificial intelligence (AI) systems are most effective when they foster collaboration with users, enabling iterative problem solving and creativity. However, many existing AI solutions operate as opaque, autonomous systems, limiting opportunities for human engagement, transparency, and refinement. This lack of integration …

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A Human-LLM Note-Taking System with Case-Based Reasoning as Framework for Scientific Discovery

Douglas B. Craig

Scientific discovery is an iterative process that requires transparent reasoning, empirical validation, and structured problem-solving.This work presents a novel human-in-the-loop AI system that leverages case-based reasoning to facilitate structured scientific inquiry.The system is designed to be note-centric, using the Obsidian note-taking application …

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What’s the data say? An LLM-based system for interrogating experimental data

Douglas B. Craig, Sorin Drăghici

Large Language Models (LLMs) have demonstrated remarkable capabilities across a variety of domains. However, issues such as hallucinations, implicit extrapolations, and inappropriate interpretations currently limit their wide adoption in scientific research. Retrieval-augmented Generation (RAG) has emerged as a promising technique to enhance …

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Single-Molecule Analysis of Alkaline Phosphatase

Douglas B. Craig

Chemical studies usually consist of measurements made on large ensembles of molecules with data representing average values for the population. It has been shown that individual molecules of a given enzyme have different properties. Large-scale averaging has in the past masked these …

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Author Correction: A novel approach for predicting upstream regulators (PURE) that affect gene expression

Tuan‐Minh Nguyen, Douglas B. Craig, Duc Tran, Tin Nguyen et autres

“SD is the founder of Advaita Bioinformatics which is commercializing iPathwayGuide, which is an IPA alternative. The method proposed in this article is not commercialized by AdvaitaBio. No funding, salaries, equipment or supplies have been received from Advaita for this work. No …

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Effect of Chemical Modification on the Distribution of Electrophoretic Mobilities of Individual Molecules of E. coli beta-Galactosidase

Brynne K Riehl, Allyster BT Klassen, Winner Pathak, Douglas B. Craig

Abstract Escherichia coli β-galactosidase was labelled with 1 mM fluorescein 5-carbamoylmethylthiopropanoic N-hydroxysuccinimidyl ester for 1 and 3 min. The samples were separated by capillary electrophoresis and peak areas compared to standards of label after attachment of BSA in order to determine the …

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A novel approach for predicting upstream regulators (PURE) that affect gene expression

Tuan‐Minh Nguyen, Douglas B. Craig, Duc Tran, Tin Nguyen et autres

External factors such as exposure to a chemical, drug, or toxicant (CDT), or conversely, the lack of certain chemicals can cause many diseases. The ability to identify such causal CDTs based on changes in the gene expression profile is extremely important in …

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Defective monocyte plasticity and altered cAMP pathway characterize USB1 ‐mutated poikiloderma with neutropenia Clericuzio type

Prahlad Parajuli, Douglas B. Craig, Manisha Gadgeel, Shruti Bagla et autres

Summary Poikiloderma with neutropenia (PN) Clericuzio type (OMIM #604173) is a rare disease with areas of skin hyper‐ and hypopigmentation caused by biallelic USB1 variants. The current study was spurred by poor healing of a perianal tear wound in one affected child …

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