Michiel van Gent

Herpes simplex virus infection promotes ALS pathology through ICP0-mediated PML body disruption

Dennis Freisem, Daniel Rombach, Sarah J. Brockmann, Anne Fink et autres

ABSTRACT Transactive response DNA binding protein 43 kDa (TDP-43) pathology, is a central molecular hallmark of amyotrophic lateral sclerosis (ALS). However, the underlying triggers are incompletely understood. Here, we show that infection with herpes simplex virus (HSV) induces molecular hallmarks of ALS …

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The dynamic interactions between virus infections and nonsense-mediated decay

Teun T. van der Klugt, Michiel van Gent

Humans are continuously exposed to a wide array of viruses that cause a significant amount of morbidity and mortality worldwide. Over recent years, the evolutionarily conserved host RNA degradation pathway nonsense-mediated decay (NMD) has emerged as a broad antiviral defense mechanism that …

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Publisher Correction: Herpesviruses mimic zygotic genome activation to promote viral replication

Eva Neugebauer, Stephanie Wälter, Tan Jiang, Nir Drayman et autres

Author notes These authors contributed equally: Eva Neugebauer, Stephanie Walter, Jiang Tan. Authors and Affiliations Institute of Virology, University Medical Center, and Faculty of Medicine, Albert-Ludwig-University Freiburg, Freiburg, Germany Eva Neugebauer, Jiang Tan, Sandra Pennisi, Pia Veratti, Karla S. Stein, Isabelle Welker …

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Herpesviruses mimic zygotic genome activation to promote viral replication

Eva Neugebauer, Stephanie Wälter, Tan Jiang, Nir Drayman et autres

Zygotic genome activation (ZGA) is crucial for maternal to zygotic transition at the 2-8-cell stage in order to overcome silencing of genes and enable transcription from the zygotic genome. In humans, ZGA is induced by DUX4, a pioneer factor that drives expression …

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Neurovirulence of Usutu virus in human fetal organotypic brain slice cultures partially resembles Zika and West Nile virus

Eleanor M. Marshall, Ahmad Rashidi, Michiel van Gent, Barry Rockx et autres

Usutu (USUV), West Nile (WNV), and Zika virus (ZIKV) are neurotropic arthropod-borne viruses (arboviruses) that cause severe neurological disease in humans. However, USUV-associated neurological disease is rare, suggesting a block in entry to or infection of the brain. We determined the replication, …

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Neurovirulence of Usutu virus in human fetal organotypic brain slice cultures resembles Zika and West Nile virus

Eleanor M. Marshall, Ahmad Rashidi, Michiel van Gent, Barry Rockx et autres

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Herpes simplex virus infection induces necroptosis of neurons and astrocytes in human fetal organotypic brain slice cultures

Ahmad Rashidi, Diana Nguyen Tran, Caithlin R. Peelen, Michiel van Gent et autres

BACKGROUND: Herpes simplex virus (HSV) encephalitis (HSE) is a serious and potentially life-threatening disease, affecting both adults and newborns. Progress in understanding the virus and host factors involved in neonatal HSE has been hampered by the limitations of current brain models that …

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Herpes simplex virus infection induces necroptosis of neurons and astrocytes in human fetal organotypic brain slice cultures

Ahmad Rashidi, Diana Nguyen Tran, Caithlin R. Peelen, Michiel van Gent et autres

Abstract Background Herpes simplex virus (HSV) encephalitis (HSE) is a serious and potentially life-threatening disease, affecting both adults and newborns. Progress in understanding the virus and host factors involved in neonatal HSE has been hampered by the limitations of current brain models …

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Varicella-zoster virus proteome-wide T-cell screening demonstrates low prevalence of virus-specific CD8 T-cells in latently infected human trigeminal ganglia

Michiel van Gent, Werner J. D. Ouwendijk, Victoria Louise Campbell, Kerry J. Laing et autres

BACKGROUND: Trigeminal ganglia (TG) neurons are an important site of lifelong latent varicella-zoster virus (VZV) infection. Although VZV-specific T-cells are considered pivotal to control virus reactivation, their protective role at the site of latency remains uncharacterized. METHODS: Paired blood and TG specimens …

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GTF3A mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand RNA5SP141

Leslie Naesens, Santoshi Muppala, Dhiraj Acharya, Josephine Nemegeer et autres

Herpes simplex virus 1 (HSV-1) infects several billion people worldwide and can cause life-threatening herpes simplex encephalitis (HSE) in some patients. Monogenic defects in components of the type I interferon system have been identified in patients with HSE, emphasizing the role of …

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Droplet-based single-cell RNA sequencing of paraformaldehyde-fixed cells

Hoang Van Phan, Michiel van Gent, Nir Drayman, Anindita Basu et autres

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High-throughput RNA sequencing of paraformaldehyde-fixed single cells

Hoang Van Phan, Michiel van Gent, Nir Drayman, Anindita Basu et autres

Single-cell transcriptomic studies that require intracellular protein staining, rare cell sorting, or inactivation of infectious pathogens are severely limited. This is because current high-throughput single-cell RNA sequencing methods are either incompatible with or necessitate laborious sample preprocessing for paraformaldehyde treatment, a common …

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