Vladislav Semak

Microgel Culture in Stirred Tank Reactors: Effects on Extracellular Vesicle Secretion and Mesenchymal Stem Cell Function

Oscar Fabian García‐Aponte, Simon Kahlenberg, Vladislav Semak, Nicolas Mytzka et autres

Three-dimensional (3D) dynamic conditions are necessary for physiologically relevant mesenchymal stem cell (MSC) culture. Furthermore, bioreactor-based processes and hydrogel-encapsulation substantially improve niche standardization, expansion control, and process scalability. The integration of these technologies can overcome some challenges that prevent the clinical translation …

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Microgel culture in stirred tank reactors: Effects on extracellular vesicle secretion and mesenchymal stem cell function

Oscar Fabian García‐Aponte, Simon Kahlenberg, Vladislav Semak, Nicolas Mytzka et autres

Three-dimensional (3D) dynamic conditions are necessary for physiologically relevant mesenchymal stem cell (MSC) culture. Furthermore, bioreactor-based processes and hydrogel-encapsulation substantially improve niche standardization, expansion control, and process scalability. The integration of these technologies can overcome some challenges that prevent the clinical translation …

Content of Short-Lived Radionuclides (125Sb, 131I, 141Ce, and 144Ce) in Fish

Nataliia E. Zarubina, Vladislav Semak, Liliia P. Ponomarenko

This review is part of a series of studies on short-lived radionuclide accumulation in aquatic organisms following nuclear weapons testing, routine facility discharges, and accidental releases. It examines the pathways of uptake, accumulation, and internal redistribution of 125Sb, 131I, 141Ce, and 144Ce …

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Content of Short-Lived Radionuclides (95Zr, 95Nb, 103Ru, and 106Ru) in Fish

Nataliia E. Zarubina, Vladislav Semak, Liliia P. Ponomarenko

This review addresses the intake, distribution, and bioaccumulation of the short-lived radionuclides 95Zr, 95Nb, 103Ru, and 106Ru in aquatic ecosystems before and after the 1986 accident at the Chornobyl Nuclear Power Plant. Particular attention is given to a detailed analysis of these …

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Content of Short-Lived Radionuclides (54Mn, 60Co, and 65Zn) in Fish

Nataliia E. Zarubina, Vladislav Semak, Liliia P. Ponomarenko, Oleg S. Burdo

This review summarizes data on the accumulation of three short-lived biogenic radionuclides—54Mn, 60Co, and 65Zn—in various fish species in the cooling pond of the Chornobyl Nuclear Power Plant in Ukraine, along with an analysis of the available literature. Significant differences exist in …

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Ecological Half-Life of 137Cs in Fish

Nataliia E. Zarubina, Vladislav Semak, Oleg S. Burdo, Liliia P. Ponomarenko

In this study, the long-term (i.e., over a 27-year period) dynamics of 137Cs content are presented for seven species of fish in both the cooling pond (CP) of the Chornobyl Nuclear Power Plant and the Kaniv Reservoir (KR). The decline of 137Cs …

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Ecological Half-Life of 137Cs in Fungi

Nataliia E. Zarubina, Vladislav Semak, Oleg S. Burdo, Liliia P. Ponomarenko

The ecological half-life of 137Cs was calculated individually for four symbiotrophic fungi species (Boletus edulis, Imleria badia, Suillus luteus, Paxillus involutus) at 10 sampling sites in the Chornobyl exclusion zone and in the Kyiv region. It was found that the maximum rate …

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Polyzwitterionic Coating of Porous Adsorbents for Therapeutic Apheresis

Vladislav Semak, Tanja Eichhorn, René Weiss, Viktoria Hoffmann Weber

Adsorbents for whole blood apheresis need to be highly blood compatible to minimize the activation of blood cells on the biomaterial surface. Here, we developed blood-compatible matrices by surface modification with polyzwitterionic polysulfobetainic and polycarboxybetainic coatings. Photoreactive zwitterionic terpolymers were synthesized by …

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Heparin-Functionalized Adsorbents Eliminate Central Effectors of Immunothrombosis, including Platelet Factor 4, High-Mobility Group Box 1 Protein and Histones

Marie Ebeyer-Masotta, Tanja Eichhorn, René Weiss, Vladislav Semak et autres

Inflammation and thrombosis are closely intertwined in numerous disorders, including ischemic events and sepsis, as well as coronavirus disease 2019 (COVID-19). Thrombotic complications are markers of disease severity in both sepsis and COVID-19 and are associated with multiorgan failure and increased mortality. …

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Comparative Analysis of Platelet-Derived Extracellular Vesicles Using Flow Cytometry and Nanoparticle Tracking Analysis

Sobha Karuthedom George, Lucia Lauková, René Weiss, Vladislav Semak et autres

Growing interest in extracellular vesicles (EVs) has prompted the advancements of protocols for improved EV characterization. As a high-throughput, multi-parameter, and single particle technique, flow cytometry is widely used for EV characterization. The comparison of data on EV concentration, however, is hindered …

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Desialylation of platelet surface glycans enhances platelet adhesion to adsorbent polymers for lipoprotein apheresis

Lucia Lauková, René Weiss, Vladislav Semak, Viktoria Hoffmann Weber

BACKGROUND: Lipoprotein apheresis is an important therapeutic option in homozygous familial hypercholesterolemia, progressive atherosclerosis, or when depletion of lipoprotein(a) is indicated. It is generally regarded as safe, but drops in platelet counts as well as sporadic episodes of thrombocytopenia have been reported. …

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Synthesis of Fluorescein Aldehydes for the Sensitive Detection of L-Cysteine

Jörg Ettenauer, Vladislav Semak, Martin Brandl

Amino acids containing thiol-groups such as Cysteine (Cys), Homocysteine (Hcy), and Glutathione play an important role in a great variety of biological processes. However, too low, but also too high concentrations can have negative effects on human health. Therefore, it is of …

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