Takao Yasui

An Unbiased Geometry‐Resolved Membrane Platform for Proteome‐Scale Discovery of Membrane Curvature Sensors

Komikawa Takumi, Rikuto Kawakami, Kunanon Chattrairat, Tatsuya Niwa et autres

Membrane curvature organizes protein-driven biochemistry on cellular membranes. Conventional membrane curvature assays are inherently limited by both candidate-based experimental designs and restricted membrane geometries, preventing proteome-scale discovery of curvature-sensing proteins. Here, we present a geometry-resolved membrane platform that enables hypothesis-agnostic identification of …

jp, us (code pays fourni par la source)

固体支持脂質二重膜を用いた逆曲率感知タンパク質の探索

児美川 拓実, Takumi Komikawa, Rikuto Kawakami, Chattrairat Kunanon et autres

Discrete polyketones enable antibody click conjugation for selective exosome profiling

Kunanon Chattrairat, Akira Yokoi, Yumehiro Manabe, Yuki Ide et autres

Targeted molecular capture from complex biological fluids remains a critical challenge in extracellular vesicle (EV) analysis. Here we develop a nanowire-based microfluidic platform functionalized via a single-step click conjugation using N -hydroxysuccinimide ester-functionalized polyketones (pKNHSs) with discrete chain lengths. Tetrameric pKNHSs enabled …

jp (code pays fourni par la source)

Overcoming charge screening via porous nanostructures for attomolar microRNA detection

Zetao Zhu, Kazuki Nagashima, Taiga Ajiri, Kunanon Chattrairat et autres

jp, th (code pays fourni par la source)

Click conjugation using discrete polyketones enables dual anchoring of antibodies and nanowires for exosome profiling

Kunanon Chattrairat, Akira Yokoi, Yumehiro Manabe, Yuki Ide et autres

Targeted molecular capture from complex biological fluids remains a critical challenge in extracellular vesicle (EV) analysis. Here we develop a nanowire-based microfluidic platform functionalized via a single-step click conjugation using N-hydroxysuccinimide-functionalized polyketones (pKNHSs) with discrete chain lengths. Tetrameric pKNHS enabled efficient and …

jp, es (code pays fourni par la source)

Estimation of α/β Ratio for Late Genitourinary Toxicity Following Salvage Radiotherapy for Biochemically Recurrent Prostate Cancer

Shingo Takano, Natsuo Tomita, Takehiro Takaoka, Minae Niwa et autres

The glomerulus as a selective gate for tumor-derived extracellular vesicles in urine

Shota Kawaguchi, Taiga Ajiri, Rina Mitsuya, Reiko Tsuchiya et autres

Abstract Urinary small extracellular vesicles (sEVs), which can reflect systemic conditions, hold great promise for non-invasive cancer diagnostics, yet the mechanism by which tumor-derived sEVs reach urine remains unclear. Here, we demonstrate that the glomerulus actively transcytoses circulating tumor-derived sEVs into urine. …

jp (code pays fourni par la source)

Nanobiodevices and quantum life science for future healthcare

Taisuke Shimada, Takao Yasui, Hiroshi Yukawa, Yoshinobu Baba

jp (code pays fourni par la source)

Selective Growth of ZnO Nanosheets via Ionic Layer Epitaxy for UV Photodetection Application

Ryunosuke Matsumura, Yuta Kazama, Hikaru Saito, Takao Yasui et autres

Zinc oxide (ZnO) nanosheets have shown great promise for ultraviolet (UV) photodetectors due to their excellent optoelectronic properties and atomically thin structure. Surfactant-assisted ionic layer epitaxy (ILE) is a simple and scalable technique for synthesizing monolayered ZnO nanosheets; however, the formation of …

jp, th (code pays fourni par la source)

Selective adsorption of unmethylated DNA on ZnO nanowires for separation of methylated DNA

Marina Musa, Zetao Zhu, Hiromi Takahashi, Wataru Shinoda et autres

DNA methylation is a crucial epigenetic modification used as a biomarker for early cancer progression. However, existing methods for DNA methylation analysis are complex, time-consuming, and prone to DNA degradation. This work demonstrates selective capture of unmethylated DNAs using ZnO nanowires without …

jp (code pays fourni par la source)

Extracellular Vesicle Isolation and Analysis Using Nanowires

Kunanon Chattrairat, Takao Yasui

Abstract Extracellular vesicles (EVs) have been considered as biomarkers for diagnosis and prognosis in therapeutic treatments. Although applications of EVs in health care are being started and more are expected, EV studies have not yet provided a deep understanding of EV biogenesis …

jp (code pays fourni par la source)

Non-toxic core–shell nanowires for in vitro extracellular vesicle scavenging

Piyawan Paisrisarn, Kunanon Chattrairat, Yuta Nakamura, Kazuki Nagashima et autres

Extracellular vesicles (EVs) from cancer cells promote abnormal growth in normal cells, potentially leading to cancer proliferation. We developed a nanowire-based EV-elimination device that efficiently eliminated EVs without toxicity. This method restored normal growth in mammary gland cells cultured with breast adenocarcinoma-derived …

jp (code pays fourni par la source)