Yohei Morishita

The Biosynthesis of a Fungal Flavonoid, Chlorflavonin, Precisely Controlled by Two Types of Equilibrium States

Sho Furumura, Taro Ozaki, Kazuya Hasegawa, Hironori Hayashi et autres

Flavonoids are polyphenolic natural products predominantly isolated from plants and exhibit a diverse array of biological activities. Because they serve as important pharmaceuticals and nutraceuticals, there is a strong demand for their sustainable supply. Chlorflavonin is a rare fungal flavonoid with potent …

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Biosynthesis of O -Prenylated Chaetophenols Triggers Nonenzymatic Pathways toward Structural Diversity

Hiroto Matsui, Yohei Morishita, Yuto Homma, Kento Tsukada et autres

Chaetophenols (CPs) are structurally diverse aromatic polyketides that have been isolated from Chaetomium fungi. They exhibit two distinct prenylation patterns that have key roles in generating chemical diversity and controlling the dimerization modes. We investigated the mechanism of CP biogenesis through heterologous …

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4-Desmethyl 7-Prenyl Mollicellin Analogue Isolated from Chaetomium brasiliense

Shuntaro Morita, Sho Furumura, Yohei Morishita, Taro Ozaki et autres

A new depsidone, termed mollicellin Z2 (1), and three known mollicellins D, H, and I (2-4), were isolated from cultured Chaetomium brasiliense NBRC 6548 mycelia. The structure of 1 was determined from one dimensional (1D) and 2D-NMR and high resolution electron ionization …

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Genome mining-based discovery of an atypical fungal non-reducing polyketide synthase encoding dimeric alkylresorcinol biosynthesis

Yuto Homma, Tomoya Hasegawa, Yohei Morishita, Soya Koremura et autres

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Discovery of a Fungal HR-PKS Cluster Encoding Biosynthetic Pathways for Macrolides with Two Distinct Ring Sizes

Yi Jie Li, Yohei Morishita, Akihiro Sugawara, Ashaimaa Y. Moussa et autres

Through the heterologous expression of a highly reducing polyketide synthase and a thioesterase from the apeml cluster, a putative macrolide biosynthetic gene cluster on the genome of Aspergillus petrakii, we obtained a naturally new 10-membered macrolide (1) and recifeiolide (2), a known …

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Vinigrol Tricyclic Scaffold Biosynthesis Employs an Atypical Terpene Cyclase and a Multipotent Cyclization Cascade

Kento Tsukada, Fumito Sato, Taro Matsuyama, Ryo Matsuda et autres

High Resolution Image Download MS PowerPoint Slide Vinigrol ( 1 ) is a fungal diterpenoid consisting of a decahydro-1,5-butanonaphthalene ring system with no analogs in nature. Despite immense efforts in synthetic studies, the vinigrol biosynthesis pathway remains largely unknown. Herein, we identified …

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Genome Mining-Based Discovery of Pyrano[2,3-c]pyrrole Type Natural Products Possessing Alkyl Side Chain with Branched Methyl Groups

Yue Shi, Taro Ozaki, Yohei Morishita, Tohru Taniguchi et autres

High Resolution Image Download MS PowerPoint Slide Genome mining of polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) in fungal endophyte Neopestalotiopsis olumideae strain BRIP 39872 identified seven new natural products (olumilides A–D, D1, D1’, D2) with pyrano[2,3- c ]pyrrole scaffolds and their biosynthetic precursor …

jp, in, fr, Ouganda, us, sg, au, it (code pays fourni par la source)

Ketosynthase Domain Catalyzes β-Lactonization in the Biosynthesis of the HMG-CoA Synthase Inhibitor Hymeglusin

Mizuki Hirokawa, Taro Ozaki, Kento Tsukada, Akihiro Sugawara et autres

High Resolution Image Download MS PowerPoint Slide Hymeglusin ( 1 ) is a fungal polyketide consisting of a β-lactone ring with a unique (3 R,4 R ) configuration. 1 is a potent 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase inhibitor. Because it circumvents drug resistance in …

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Biosynthesis of Circumdatins Employs an Anthranilate Tailoring Pathway for NRPS Substrate Supplies

Yohei Morishita, Yuto Homma, Akihiro Sugawara, Ashaimaa Y. Moussa et autres

Circumdatins are a unique class of fungal alkaloids featuring a pyrrolo[4,2]benzodiazepine scaffold. In this study, we identified the biosynthetic gene cluster through genome mining guided by structural analysis and docking simulations. Heterologous expression and precursor feeding experiments revealed an unprecedented tailoring pathway …

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Peptide Recognition and Mechanism of the Radical S-Adenosyl- l -methionine Multiple Cyclophane Synthase ChlB

Jérémie Ruel, Thi Quynh Ngoc Nguyen, Yohei Morishita, Anthony Usclat et autres

Ribosomally synthesized and post-translationally modified peptides (RiPPs) represent a valuable class of natural products, often featuring macrocyclization, which enhances stability and rigidity to achieve specific conformations, frequently underlying antibiotic activity. ChlB is a metalloenzyme with two catalytic domains─a radical S -adenosyl- l …

fr, vn, sg, jp (code pays fourni par la source)

Author Correction: Fused radical SAM and αKG-HExxH domain proteins contain a distinct structural fold and catalyse cyclophane formation and β-hydroxylation

Yohei Morishita, Suze Ma, E. De la Mora, He Li et autres

sg, jp, cn, fr (code pays fourni par la source)

Discovery and Theoretical Studies of Nonenzymatic Polyketide Dimerizations of Chaetophenols

Hiroto Matsui, Yohei Morishita, Takashi Yamamoto, Taro Ozaki et autres

High Resolution Image Download MS PowerPoint Slide Nonenzymatic reactions sometimes construct complex structures observed in natural products, showing us unique chemical reactions. In our exploration of natural products, we identified a novel type of isochromene-derived polyketide dimer 7 with a 6/6/6/6/6 five-ring …

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