Grant Ganshaw

Structure of a Novel Enzyme That Catalyzes Acyl Transfer to Alcohols in Aqueous Conditions

Irimpan I. Mathews, M. Soltis, Mae Saldajeno, Grant Ganshaw et autres

The unusual architecture of the enzyme (MsAcT) isolated from Mycobacterium smegmatis forms the mechanistic basis for favoring alcoholysis over hydrolysis in water. Unlike hydrolases that perform alcoholysis only under anhydrous conditions, MsAcT demonstrates alcoholysis in substantially aqueous media and, in the presence …

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Structure of the Native M. Smegmatis Aryl Esterase

Irimpan I. Mathews, M. Soltis, Mae Saldajeno, Grant Ganshaw et autres

Structure of the Inhibitor bound form of M. Smegmatis Aryl Esterase

Irimpan I. Mathews, M. Soltis, Mae Saldajeno, Grant Ganshaw et autres

A Bacillus subtilis fusion protein system to produce soybean Bowman–Birk protease inhibitor

Gudrun Vogtentanz, Katherine D. Collier, Michael Bodó, Judy H. Chang et autres

A silicone‐based controlled‐release device for accelerated proteolytic debridement of wounds

R. H. Bott, James W. Crissman, Csilla Kollar, Mae Saldajeno et autres

A new device for rapid enzymatic debridement of cutaneous wounds has been developed using a controlled-release, silicone-based, dried emulsion. A dehydrated serine protease of the subtilisin family, previously untested for wound debridement, was incorporated into the emulsion. This device exhibited excellent storage …

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Structure of a novel acyltransferase

R.C. Bott, Mae Saldajeno, Grant Ganshaw, Marguerite A. Cervin et autres

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Comparative NMR study on the impact of point mutations on protein stability of Pseudomonas mendocina lipase

Nathalie Sibille, Adrien Favier, Ana I. Azuaga, Grant Ganshaw et autres

In this work we compare the dynamics and conformational stability of Pseudomonas mendocina lipase enzyme and its F180P/S205G mutant that shows higher activity and stability for use in washing powders. Our NMR analyses indicate virtually identical structures but reveal remarkable differences in …

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Do Enzymes Change the Nature of Transition States? Mapping the Transition State for General Acid−Base Catalysis of a Serine Protease

R. Bott, Gina Chan, Blanca Domingo, Grant Ganshaw et autres

The properties of the transition state for serine protease-catalyzed hydrolysis of an amide bond were determined for a series of subtilisin variants from Bacillus lentus. There is no significant change in the structure of the enzyme upon introduction of charged mutations S156E/S166D, …

S156E/S166D variant of Bacillus lentus subtilisin

R. Bott, Giek Far Chan, Beatriz Domingo, Grant Ganshaw et autres

Enhanced immunogenicity of a functional enzyme by T cell epitope modification

Jeanette Mucha, Marcia M Stickler, AJ Poulose, Grant Ganshaw et autres

BACKGROUND: T helper epitopes are necessary for the induction of high titers of antigen-specific IgG antibodies. We are interested in the epitope modification of intact proteins as a method to enhance their immunogenicity for the generation of recombinant protein-based vaccines. RESULTS: Hartley …

Snapshots of Enzyme Activation

R. Bott, Grant Ganshaw, M. Soltis, Peter Kühn et autres

Crystals of B. lentus subtilisin, when cryo-cooled diffract to 0.85 Å resolution or better, which has been sufficient to resolve a special hydrogen bond within the catalytic triad at pH 5.9 (1) We have collected data from crystals at pH 5.0 and …

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Engineered Bacillus lentus subtilisins having altered flexibility 1 1Edited by P. E. Wright

Thomas P. Graycar, Mark Knapp, Grant Ganshaw, JUDY L. DAUBERMAN et autres