А. В. Лавров

Transcriptomic Profile of Directed Differentiation of iPSCs into Hepatocyte-like Cells

Irina Panchuk, Valeriia Kovalskaia, Konstantin S. Kochergin-Nikitsky, Valentina D. Yakushina et autres

The liver is the central organ in metabolism; however, modeling hepatic diseases remains limited by current experimental models. Animal models frequently fail to predict human liver physiology, while primary hepatocytes rapidly dedifferentiate in culture. We performed comprehensive transcriptomic profiling of induced pluripotent …

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The Promise and Pitfalls of AAV-Mediated Gene Therapy for Duchenne Muscular Dystrophy

Elizaveta V. Kurshakova, Olga A. Levchenko, Svetlana Smirnikhina, А. В. Лавров

gene encoding the dystrophin protein. The absence of functional dystrophin leads to destabilization of the dystrophin-associated glycoprotein complex (DAPC), sarcolemmal damage, and progressive degeneration of muscle fibers. Current therapeutic strategies focus on restoring dystrophin expression using genome editing approaches. Adeno-associated virus (AAV) …

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Methodological landscape in the field of integration site identification of retroviruses and retroviral vectors

Konstantin S. Kochergin-Nikitsky, А. В. Лавров, Svetlana Smirnikhina

Detailed mapping of viral vector integration sites, including retroviral and particularly lentiviral vectors, is critical for assessing their safety in preclinical and clinical studies. Although integration into the host genome follows certain virus-specific patterns, it remains a stochastic event and can cause …

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Prime Editing Modification with FEN1 Improves F508del Variant Editing in the CFTR Gene in Airway Basal Cells

Olga V. Volodina, Anna G. Demchenko, Arina A. Anuchina, О. П. Рыжкова et autres

Prime editing is a promising approach for correcting pathogenic variants, but its efficiency remains variable across genomic contexts. Here, we systematically evaluated 12 modifications of the PEmax system for correcting the CFTR F508del pathogenic variant that caused cystic fibrosis in patient-derived airway …

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P043 Innovative approaches in cystic fibrosis: development of prime editing-based gene therapy for F508del CFTR variant

Olga V. Volodina, Anna G. Demchenko, Arina A. Anuchina, О. П. Рыжкова et autres

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Selection of Optimal pegRNAs to Enhance Efficiency of Prime Editing in AT-Rich Genome Regions

Olga V. Volodina, Anna G. Demchenko, Arina A. Anuchina, О. П. Рыжкова et autres

Abstract Prime editing is a highly promising strategy for treating hereditary disorders due to its superior efficiency and safety profile compared to the conventional CRISPR-Cas9 systems. This study is dedicated to development of a causal therapy for cystic fibrosis by targeting the …

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Lung Organoids from hiPSCs Can Be Efficiently Transduced by Recombinant Adeno-Associated Viral and Adenoviral Vectors

Lyubava Belova, Anna G. Demchenko, Anastasia Erofeeva, Konstantin S. Kochergin-Nikitsky et autres

Background: Organoids are a valuable model for studying hereditary diseases such as cystic fibrosis (CF). Recombinant adenoviral (rAdV) and adeno-associated viral (rAAV) vectors are promising tools for CF gene therapy and genome editing. Objective: This study aims to determine the most efficient …

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The youngest known European Promephitis (Carnivora: Mephitidae): a new discovery from the Early Pliocene of Moldova

I. F. Araslanov, А. В. Лавров, Gennady F. Baryshnikov, Alexander O. Averianov

Mephitidae is one of the most enigmatic Carnivora (Mammalia) group. They originated in the early–middle Oligocene of Asia according to molecular studies. However, their first fossil appearance is dated to the Early Miocene. Mephitidae has one of the longest gaps in the …

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Challenges in developing gene therapy against Duchenne muscular dystrophy

Elizaveta V. Kurshakova, Olga A. Levchenko, А. В. Лавров

Duchenne muscular dystrophy is a progressive X-linked recessive neuromuscular disorder resulting from pathogenic mutations in the DMD gene, which codes dystrophin. It is one of the essential structural proteins of muscle cells that maintains the integrity of cross-striated muscles. Duchenne muscular dystrophy …

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Helminth Eggs from Pachycrocuta Brevirostris (Carnivora, Hyaenidae) Coprolites from Taurida Cave (Early Pleistocene, Crimea)

Tatiana N. Sivkova, Daniyar R. Khantemirov, Д. О. Гимранов, А. В. Лавров

Twenty-seven Pachycrocuta brevirostris coprolites from Taurida Cave (Early Pleistocene) were studied. Eggs of parasitic worms were found in 6 of them (22.2%). Eggs of Trematoda species were identified among them, as were eggs of Taenia, Toxocara, and Capillaria. Toxocara eggs were the …

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Etruscan wolf Canis etruscus (Canidae, Carnivora) from the Early Pleistocene of Crimea (Taurida cave)

А. В. Лавров, Marina Vladimirovna Sotnikova, Д. О. Гимранов, Joan Madurell‐Malapeira et autres

The cranial and mandibular fragments of the medium-sized canid from the Early Pleistocene locality of Taurida cave (about 1.8–1.5 Ma) in Crimea are described and included in the hypodigm of the species Canis etruscus Forsyth Major, 1877, a common member of the …

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Cheetah Acinonyx pardinensis (Felidae, Carnivora)from the Early Pleistocene of Crimea (Taurida cave)

Д. О. Гимранов, Joan Madurell‐Malapeira, Qigao Jiangzuo, А. В. Лавров et autres

A mandible fragment of Acinonyx pardinensis (Croizet et Jobert, 1828) is described from the Early Pleistocene locality in the Taurida cave (Crimea, Late Villafranchian, about 1.8–1.5 Ma). This is the first discovery of the genus Acinonyx in the Pleistocene of Crimea. Along …

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