Sven Schuierer

Simian iPS-derived hepatocytes support Plasmodium cynomolgi infection and hypnozoite formation

Mélanie Pellisson, Anne‐Marie Zeeman, Thierry Doll, Lucy Kirchhofer-Allan et autres

Introduction Plasmodium cynomolgi is one of the few malaria parasite species that forms dormant liver stages known as hypnozoites and is therefore a suitable model for Plasmodium vivax , the causative agent of recurrent malaria. Very little is known about the biology …

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Single cell multi-omics reveals re-dosing with CD3 bispecific antibody induces a TCF7 high central memory CD8 + T cell population associated with reduced cytokine production

Ling Hui, Lauren C. Anderson, Katie Kubek-Luck, Juliane Perner et autres

T cell engaging therapies are commonly accompanied by excessive cytokine production and risk of cytokine release syndrome (CRS). Intriguingly, CRS risk with CD3-engaging bispecific antibody (BSP) is primarily limited to the first dose, termed the first-dose effect. Mechanisms underlying this effect remain …

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Disruption of serotonin homeostasis in intestinal organoids provides insights into drug-induced gastrointestinal toxicity

Georgia M Rouseti, Audrey Fischer, Nicole Rathfelder, Annick Waldt et autres

Drug-induced gastrointestinal toxicity is a frequent clinical adverse event that needs to be carefully monitored and managed to ensure patient compliance. While preclinical assessment of drug-induced gastrointestinal toxicity mostly relies on animal experimentation, intestinal organoids have gained increasing attention to identify gastrointestinal …

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Molecular characterization of chronic cutaneous wounds reveals subregion‐ and wound type‐specific differential gene expression

Shola M. Richards, Caroline Gubser Keller, Robert Kreutzer, Géraldine Greiner et autres

A limited understanding of the pathology underlying chronic wounds has hindered the development of effective diagnostic markers and pharmaceutical interventions. This study aimed to elucidate the molecular composition of various common chronic ulcer types to facilitate drug discovery strategies. We conducted a …

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Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi

Gabriel Mitchell, Guglielmo Roma, Annemarie Voorberg-van der Wel, Martin Beibel et autres

BACKGROUND: The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously reported and …

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Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi

Gabriel Mitchell, Guglielmo Roma, Annemarie Voorberg-van der Wel, Martin Beibel et autres

ABSTRACT The zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. We previously reported the transcriptional profiling of P. cynomolgi liver stages and …

us, ch, nl (code pays fourni par la source)

Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq

Vincent Hahaut, Dinko Pavlinić, Walter Carbone, Sven Schuierer et autres

We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease …

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Probabilistic machine learning ensures accurate ambient denoising in droplet-based single-cell omics

Caibin Sheng, Rui Lopes, Gang Li, Sven Schuierer et autres

Abstract Droplet-based single-cell omics, including single-cell RNA sequencing (scRNAseq), single-cell CRISPR perturbations (e.g., CROP-seq), and single-cell protein and transcriptomic profiling (CITE-seq) hold great promise for comprehensive cell profiling and genetic screening at the single-cell resolution. However, these technologies suffer from substantial noise, …

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Persistence of Plasmodium cynomolgi hypnozoites in cynomolgus monkey iPS-derived hepatocytes

Mélanie Pellisson, Anne‐Marie Zeeman, Thierry Doll, Lucy Kirchhofer-Allan et autres

Abstract Plasmodium cynomolgi (Pc) is one of the few parasite species that forms quiescent liver stage parasites known as hypnozoites and is therefore a suitable model for Plasmodium vivax . Very little is known about liver stage dormancy, which hampers the search …

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Single-cell and bulk transcriptomics of the liver reveals potential targets of NASH with fibrosis

Zhongyi Wang, Adrian Keogh, Annick Waldt, Rachel Cuttat et autres

Fibrosis is characterized by the excessive production of collagen and other extracellular matrix (ECM) components and represents a leading cause of morbidity and mortality worldwide. Previous studies of nonalcoholic steatohepatitis (NASH) with fibrosis were largely restricted to bulk transcriptome profiles. Thus, our …

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ZNRF3 and RNF43 cooperate to safeguard metabolic liver zonation and hepatocyte proliferation

Tianliang Sun, Stefano Annunziato, Sebastian Bergling, Caibin Sheng et autres

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A Simple and Efficient CRISPR Technique for Protein Tagging

Fanning Zeng, Valérie Beck, Sven Schuierer, Isabelle Garnier et autres

Genetic knock-in using homology-directed repair is an inefficient process, requiring the selection of few modified cells and hindering its application to primary cells. Here, we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by CRISPR in …

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