Raffaela S. Berger

Correction: Fadil et al. Isotope Ratio Outlier Analysis (IROA) for HPLC–TOFMS-Based Metabolomics of Human Urine. Metabolites 2022, 12, 741

Fadi Fadil, Claudia Samol, Raffaela S. Berger, Fabian Kellermeier et autres

It was pointed out to us that we had not followed exactly the IROA TruQuant IQQ Workflow Kit protocol in the experimental part of our work [...].

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D-2-hydroxyglutarate supports a tolerogenic phenotype with lowered major histocompatibility class II expression in non-malignant dendritic cells and acute myeloid leukemia cells

Kathrin Hammon, Kathrin Renner, Michael Althammer, Florian Voll et autres

D-2-hydroxyglutarate (D-2-HG) accumulates in patients with acute myeloid leukemia (AML) with mutated isocitrate dehydrogenase (IDH) and in other malignancies. D-2-HG suppresses antitumor T-cell immunity but little is known about potential effects on non-malignant myeloid cells. Here we show that D-2-HG impairs human …

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Macrophages inhibit Coxiella burnetii by the ACOD1‐itaconate pathway for containment of Q fever

Lisa Kohl, Md Nur A Alam Siddique, Barbara Bodendorfer, Raffaela S. Berger et autres

Abstract Infection with the intracellular bacterium Coxiella (C.) burnetii can cause chronic Q fever with severe complications and limited treatment options. Here, we identify the enzyme cis‐aconitate decarboxylase 1 (ACOD1 or IRG1) and its product itaconate as protective host immune pathway in …

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Isotope Ratio Outlier Analysis (IROA) for HPLC–TOFMS-Based Metabolomics of Human Urine

Fadi Fadil, Claudia Samol, Raffaela S. Berger, Fabian Kellermeier et autres

Metabolic fingerprinting by mass spectrometry aims at the comprehensive, semiquantitative analysis of metabolites. Isotope dilution, if successfully implemented, may provide a more reliable, relative quantification. Therefore, the 13C labeled yeast extract of the IROA TruQuant kit was added as an internal standard …

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Macrophages inhibit Coxiella burnetii by the ACOD1-itaconate pathway for containment of Q fever

Lisa Kohl, Md Nur A Alam Siddique, Barbara Bodendorfer, Raffaela S. Berger et autres

Abstract Infection with the intracellular bacterium Coxiella (C.) burnetii can cause chronic Q fever with severe complications and limited treatment options. Here, we identify the enzyme cis- aconitate decarboxylase 1 (ACOD1 or IRG1) and its product itaconate as protective host immune pathway …

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Acidic Microenvironments Found in Cutaneous Leishmania Lesions Curtail NO-Dependent Antiparasitic Macrophage Activity

Linus Frick, Linda Hinterland, Kathrin Renner, Marion Vogl et autres

Local tissue acidosis affects anti-tumor immunity. In contrast, data on tissue pH levels in infected tissues and their impact on antimicrobial activity is sparse. In this study, we assessed the pH levels in cutaneousLeishmanialesions.Leishmania major-infected skin tissue displayed pH levels of 6.7 …

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Empagliflozin Reduces Renal Hyperfiltration in Response to Uninephrectomy, but Is Not Nephroprotective in UNx/DOCA/Salt Mouse Models

Philipp Tauber, Frederick Sinha, Raffaela S. Berger, Wolfram Gronwald et autres

Large-scale clinical outcome studies demonstrated the efficacy of SGLT2 inhibitors in patients with type II diabetes. Besides their therapeutic efficacy in diabetes, significant renoprotection was observed in non-diabetic patients with chronic kidney disease (CKD), suggesting the existence of glucose-independent beneficial effects of …

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Kynurenine induces T cell fat catabolism and has limited suppressive effects in vivo

Peter J. Siska, Jing Jiao, Carina Matos, Katrin Singer et autres

Background L-kynurenine is a tryptophan-derived immunosuppressive metabolite and precursor to neurotoxic anthranilate and quinolinate. We evaluated the stereoisomer D-kynurenine as an immunosuppressive therapeutic which is hypothesized to produce less neurotoxic metabolites than L-kynurenine. Methods L-/D-kynurenine effects on human and murine T cell …

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Lactonization of the Oncometabolite D-2-Hydroxyglutarate Produces a Novel Endogenous Metabolite

Raffaela S. Berger, Christian J. Wachsmuth, Magdalena C. Waldhier, Kathrin Renner et autres

In recent years, onco-metabolites like D-2-hydroxyglutarate, which is produced in isocitrate dehydrogenase-mutated tumors, have gained increasing interest. Here, we report a metabolite in human specimens that is closely related to 2-hydroxyglutarate: the intramolecular ester of 2-hydroxyglutarate, 2-hydroxyglutarate-γ-lactone. Using 13C5-L-glutamine tracer analysis, we …

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Optimized Protocol for the In Situ Derivatization of Glutathione with N-Ethylmaleimide in Cultured Cells and the Simultaneous Determination of Glutathione/Glutathione Disulfide Ratio by HPLC-UV-QTOF-MS

Xueni Sun, Raffaela S. Berger, Paul V. Heinrich, Ibtissam Marchiq et autres

Glutathione (GSH) and glutathione disulfide (GSSG) are commonly used to assess the oxidative status of a biological system. Various protocols are available for the analysis of GSH and GSSG in biomedical specimens. In this study, we present an optimized protocol for the …

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Development and application of analytical tools to study the origin, fate and impact of the oncometabolite 2-hydroxyglutarate and its lactone

Raffaela S. Berger

This thesis investigated the impact and metabolism of the known oncometabolite D 2 hydroxyglutarate and its recently recognized lactone R-5-oxo-2-tetrahydro-furancarboxylic acid. Production and biological effects of 2 hydoxyglutarate are well described in the literature. However, accumulation of this metabolite as a result …

Quantification and 13C-Tracer analysis of total reduced glutathione by HPLC-QTOFMS/MS

Xueni Sun, Paul V. Heinrich, Raffaela S. Berger, Peter J. Oefner et autres

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