Tim M. van Leeuwe

Deletion of the Aspergillus niger Pro-Protein Processing Protease Gene kexB Results in a pH-Dependent Morphological Transition during Submerged Cultivations and Increases Cell Wall Chitin Content

Tim M. van Leeuwe, Mark Arentshorst, Gabriel Forn‐Cuní, Nicholas Geoffrion et autres

There is a growing interest in the use of post-fermentation mycelial waste to obtain cell wall chitin as an added-value product. In the pursuit to identify suitable production strains that can be used for post-fermentation cell wall harvesting, we turned to an …

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Chitin in the fungal cell wall: Towards valorization of spent biomass of Aspergillus niger

Tim M. van Leeuwe

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass. In the European Research Area Industrial Biotechnology (ERA-IB) funded project, we effectively aimed to improve the composition of post-fermentation fungal biomass for extraction …

A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan

Tim M. van Leeuwe, Jasper Wattjes, Anna Niehues, Gabriel Forn‐Cuní et autres

Chitin is an important fungal cell wall component that is cross-linked to β-glucan for structural integrity. Acquisition of chitin to glucan cross-links has previously been shown to be performed by transglycosylation enzymes in Saccharomyces cerevisiae, called Congo Red hypersensitive (Crh) enzymes. Here, …

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Interrogation of the cell wall integrity pathway in Aspergillus niger identifies a putative negative regulator of transcription involved in chitin deposition

Tim M. van Leeuwe, Mark Arentshorst, Peter J. Punt, Arthur F. J. Ram

Post-fermentation fungal biomass waste provides a viable source for chitin. Cell wall chitin of filamentous fungi, and in particular its de-N-acetylated derivative chitosan, has a wide range of commercial applications. Although the cell wall of filamentous fungi comprises 10-30% chitin, these yields …

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Rab GDP-dissociation inhibitor gdiA is an essential gene required for cell wall chitin deposition in Aspergillus niger

Tim M. van Leeuwe, Anne Gerritsen, Mark Arentshorst, Peter J. Punt et autres

The cell wall is a distinctive feature of filamentous fungi, providing them with structural integrity and protection from both biotic and abiotic factors. Unlike plant cell walls, fungi rely on structurally strong hydrophobic chitin core for mechanical strength together with alpha- and …

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Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

Tim M. van Leeuwe, Mark Arentshorst, Tim Ernst, Ebru Alazi et autres

Abstract Background CRISPR/Cas9 mediated genome editing has expedited the way of constructing multiple gene alterations in filamentous fungi, whereas traditional methods are time-consuming and can be of mutagenic nature. These developments allow the study of large gene families that contain putatively redundant …

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The evolution of no-cost resistance at sub-MIC concentrations of streptomycin in Streptomyces coelicolor

Sanne Westhoff, Tim M. van Leeuwe, Omar I. Qachach, Zheren Zhang et autres

At the high concentrations used in medicine, antibiotics exert strong selection on bacterial populations for the evolution of resistance. However, these lethal concentrations may not be representative of the concentrations bacteria face in soil, a recognition that has led to questions of …

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The evolution of no-cost resistance at sub-MIC concentrations of streptomycin in Streptomyces coelicolor

Sanne Westhoff, Tim M. van Leeuwe, Omar I. Qachach, Zheren Zhang et autres

Abstract At the high concentrations used in medicine, antibiotics exert strong selection on bacterial populations for the evolution of resistance. However, these lethal concentrations may not be representative of the concentrations bacteria face in soil, a recognition that has lead to questions …

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The evolution of no-cost resistance at sub-MIC concentrations of

Sanne Westhoff, Tim M. van Leeuwe, Omar I. Qachach, Zheren Zhang et autres