Ebru Alazi

Loss of function of the carbon catabolite repressor CreA leads to low but inducer‐independent expression from the feruloyl esterase B promoter in Aspergillus niger

Jos Reijngoud, Mark Arentshorst, Claudine W.B. Ruijmbeek, Ian D. Reid et autres

OBJECTIVE: With the aim to decipher the mechanisms involved in the transcriptional regulation of feruloyl esterase encoded by faeB, a genetic screen was performed to isolate A. niger mutants displaying inducer-independent expression from the faeB promoter. RESULT: PfaeB-amdS and PfaeB-lux dual reporter …

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Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi

Tim M. van Leeuwe, Mark Arentshorst, Tim Ernst, Ebru Alazi et autres

Abstract Background CRISPR/Cas9 mediated genome editing has expedited the way of constructing multiple gene alterations in filamentous fungi, whereas traditional methods are time-consuming and can be of mutagenic nature. These developments allow the study of large gene families that contain putatively redundant …

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Functional analysis of three putative galactofuranosyltransferases with redundant functions in galactofuranosylation in Aspergillus niger

Mark Arentshorst, Davina de Lange, Joohae Park, Ellen Lagendijk et autres

Galactofuranose (Galf)-containing glycostructures are important to secure the integrity of the fungal cell wall. Golgi-localized Galf-transferases (Gfs) have been identified in Aspergillus nidulans and Aspergillus fumigatus. BLASTp searches identified three putative Galf-transferases in Aspergillus niger. Phylogenetic analysis showed that they group in …

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Correction to: Mutations in AraR leading to constitutive expression of arabinolytic genes in Aspergillus niger under derepressing conditions

Jos Reijngoud, Malte Deseke, Elmar T. M. Halbesma, Ebru Alazi et autres

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Mutations in AraR leading to constitutive of arabinolytic genes in Aspergillus niger under derepressing conditions

Jos Reijngoud, Malte Deseke, Elmar T. M. Halbesma, Ebru Alazi et autres

The AraR transcription factor of Aspergillus niger encodes a Zn(II) 2 Cys 6 transcription factor required for the induction of genes encoding arabinolytic enzymes. One of the target genes of AraR is abfA , encoding an arabinofuranosidase. The expression of abfA as …

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W361R mutation in GaaR, the regulator of D‐galacturonic acid‐responsive genes, leads to constitutive production of pectinases in Aspergillus niger

Ebru Alazi, Jing Niu, Simon B. Otto, Mark Arentshorst et autres

Abstract Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi. Aspergillus niger naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D‐galacturonic acid. The transcriptional activator GaaR, the repressor of D‐galacturonic acid …

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Modulating Transcriptional Regulation of Plant Biomass Degrading Enzyme Networks for Rational Design of Industrial Fungal Strains

Ebru Alazi, Arthur F. J. Ram

Filamentous fungi are the most important microorganisms for the industrial production of plant polysaccharide degrading enzymes due to their unique ability to secrete these proteins efficiently. These carbohydrate active enzymes (CAZymes) are utilized industrially for the hydrolysis of plant biomass for the …

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Discovery and exploitation of the transcriptional regulatory system of pectinases in Aspergillus niger

Ebru Alazi

Pectin is\na plant cell wall polysaccharide made of mainly D-galacturonic acid (GA)\nsubunits. The potency of the filamentous fungus Aspergillus niger to naturally\nsecrete high amounts of pectinases to degrade pectin has been utilized for the\nindustrial production of pectinases. Industrially produced pectinases by A.\nniger …

Inducer-independent production of pectinases in Aspergillus niger by overexpression of the D-galacturonic acid-responsive transcription factor gaaR

Ebru Alazi, Tim G J Knetsch, Marcos Di Falco, Ian D. Reid et autres

The transcription factor GaaR is needed for the expression of genes required for pectin degradation and transport and catabolism of the main degradation product, D-galacturonic acid (GA) in Aspergillus niger. In this study, we used the strong constitutive gpdA promoter of Aspergillus …

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The pathway intermediate 2‐keto‐3‐deoxy‐L‐galactonate mediates the induction of genes involved in D‐galacturonic acid utilization in Aspergillus niger

Ebru Alazi, Claire Khosravi, Tim Homan, Saskia du Pré et autres

In Aspergillus niger, the enzymes encoded by gaaA, gaaB, and gaaC catabolize d-galacturonic acid (GA) consecutively into l-galactonate, 2-keto-3-deoxy-l-galactonate, pyruvate, and l-glyceraldehyde, while GaaD converts l-glyceraldehyde to glycerol. Deletion of gaaB or gaaC results in severely impaired growth on GA and accumulation …

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An Evolutionarily Conserved Transcriptional Activator-Repressor Module Controls Expression of Genes for D-Galacturonic Acid Utilization in Aspergillus niger

Jing Niu, Ebru Alazi, Ian D. Reid, Mark Arentshorst et autres

The expression of genes encoding extracellular polymer-degrading enzymes and the metabolic pathways required for carbon utilization in fungi are tightly controlled. The control is mediated by transcription factors that are activated by the presence of specific inducers, which are often monomers or …

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Transcriptomic and molecular genetic analysis of the cell wall salvage response ofAspergillus nigerto the absence of galactofuranose synthesis

Joohae Park, Mark Hulsman, Mark Arentshorst, Matthijs Breeman et autres

The biosynthesis of cell surface-located galactofuranose (Galf)-containing glycostructures such as galactomannan, N-glycans and O-glycans in filamentous fungi is important to secure the integrity of the cell wall. UgmA encodes an UDP-galactopyranose mutase, which is essential for the formation of Galf. Consequently, the …

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