Christen L. Grassel

The role of CFA/I in adherence and toxin delivery by ETEC expressing multiple colonization factors in the human enteroid model

Emily M. Smith, Christen L. Grassel, Antonia Papadimas, Jennifer D. Foulke-Abel et autres

Enterotoxigenic Escherichia coli (ETEC) is a primary causative agent of diarrhea in travelers and young children in low-to-middle-income countries (LMICs). ETEC adhere to intestinal epithelia via colonization factors (CFs) and secrete heat-stable toxin (ST) and/or heat-labile toxin (LT), causing dysregulated cellular ion …

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Evaluation of a Live Attenuated S. sonnei Vaccine Strain in the Human Enteroid Model

Giulia Pilla, Tao Wu, Christen L. Grassel, Jonathan Moon et autres

Shigella is a leading cause of bacillary dysentery worldwide, responsible for high death rates especially among children under five in low–middle income countries. Shigella sonnei prevails in high-income countries and is becoming prevalent in industrializing countries, where multi-drug resistant strains have emerged, …

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Characterization of Schu S4 aro mutants as live attenuated tularemia vaccine candidates

Aimee L. Cunningham, Barbara J. Mann, Aiping Qin, Araceli E. Santiago et autres

There is a need for development of an effective vaccine against Francisella tularensis, as this potential bioweapon has a high mortality rate and low infectious dose when delivered via the aerosol route. Moreover, this Tier 1 agent has a history of weaponization. …

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Characterization of Schu S4 aro mutants as live attenuated tularemia vaccine candidates

Aimee L. Cunningham, Barbara J. Mann, Aiping Qin, Araceli E. Santiago et autres

There is a need for development of an effective vaccine against Francisella tularensis, as this potential bioweapon has a high mortality rate and low infectious dose when delivered via the aerosol route. Moreover, this Tier 1 agent has a history of weaponization. …

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Evaluating Shigella flexneri Pathogenesis in the Human Enteroid Model

Sridevi Ranganathan, Michele Y Doucet, Christen L. Grassel, BreOnna Delaine-Elias et autres

The enteric pathogen Shigella is one of the leading causes of moderate-to-severe diarrhea and death in young children in developing countries. Transformed cell lines and animal models have been widely used to study Shigella pathogenesis.

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Biochemical and Immunological Evaluation of Recombinant CS6-Derived Subunit Enterotoxigenic Escherichia coli Vaccine Candidates

S. Poole, Milton Maciel, Premkumar Dinadayala, Kathleen E. Dori et autres

(ETEC) isolates, is comprised of two major subunit proteins, CssA and CssB. Using donor strand complementation, we constructed a panel of recombinant proteins of 1 to 3 subunits that contained combinations of CssA and/or CssB subunits and a donor strand, a C-terminal …

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Deletion of the Major Facilitator Superfamily Transporter fptB Alters Host Cell Interactions and Attenuates Virulence of Type A Francisella tularensis

Phillip M. Balzano, Aimee L. Cunningham, Christen L. Grassel, Eileen M. Barry

ABSTRACT Francisella tularensis is a Gram-negative, facultative, intracellular coccobacillus that can infect a wide variety of hosts. In humans, F. tularensis causes the zoonosis tularemia following insect bites, ingestion, inhalation, and the handling of infected animals. The fact that a very small …

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The synthesis of OspD3 (ShET2) inShigella flexneriis independent of OspC1

Christina S. Faherty, Tao Wu, Carolyn R. Morris, Christen L. Grassel et autres

Shigella flexneri is a Gram-negative pathogen that invades the colonic epithelium and causes millions of cases of watery diarrhea or bacillary dysentery predominately in children under the age of 5 years in developing countries. The effector Shigella enterotoxin 2 (ShET2), or OspD3, …

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Characterization of a multicomponent live, attenuatedShigella flexnerivaccine

BreOnna C DeLaine, Tao Wu, Christen L. Grassel, Avital A. Shimanovich et autres

Shigella flexneri is a leading cause of diarrheal disease in children under five in developing countries. There is currently no licensed vaccine and broad spectrum protection requires coverage of multiple serotypes. The live attenuated vaccines CVD 1213 and CVD 1215 were derived …

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Simple method for purification of enterotoxigenic Escherichia coli fimbriae

Brittany Curtis, Christen L. Grassel, Rachel S. Laufer, Khandra T. Sears et autres

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Characterization of Francisella tularensis Schu S4 defined mutants as live-attenuated vaccine candidates

Araceli E. Santiago, Barbara J. Mann, Aiping Qin, Aimee L. Cunningham et autres

Francisella tularensis (Ft), the etiological agent of tularemia and a Tier 1 select agent, has been previously weaponized and remains a high priority for vaccine development. Ft tularensis (type A) and Ft holarctica (type B) cause most human disease. We selected six …

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Mo1941 Further Characterization of the Shigella Flexneri Effector OspD3 (ShET2) Reveals A Unique Expression Profile That Does Not Require OspC1

Christina S. Faherty, Tao Wu, Carolyn R. Morris, Christen L. Grassel et autres