Matthias Zscharnack

Point-of-care treatment of focal cartilage defects with selected chondrogenic mesenchymal stromal cells-An in vitro proof-of-concept study

Oliver Petters, Christian Schmidt, Christian B Thuemmler, Frank Peinemann et autres

Due to the poor self-healing capacities of cartilage, innovative approaches are a major clinical need. The use of in vitro expanded mesenchymal stromal cells (MSCs) in a 2-stage approach is accompanied by cost-, time-, and personnel-intensive good manufacturing practice production. A 1-stage …

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Preclinical good laboratory practice-compliant safety study to evaluate biodistribution and tumorigenicity of a cartilage advanced therapy medicinal product (ATMP)

Matthias Zscharnack, Christoph Krause, Gabriela Aust, Christian Thümmler et autres

BACKGROUND: The clinical development of advanced therapy medicinal products (ATMPs), a new class of drugs, requires initial safety studies that deviate from standard non-clinical safety protocols. The study provides a strategy to address the safety aspects of biodistribution and tumorigenicity of ATMPs …

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Isolation and Characterization of CD271+ Stem Cells Derived from Sheep Dermal Skin

Roomina Jahroomishirazi, Augustinus Bader, Sabine Ebert, Christian Schmidt et autres

BACKGROUND: Mesenchymal stem cells (MSCs) have great promise in the field of regenerative medicine due to their differentiation potential into several lineages. Besides the bone marrow, MSCs can be obtained from the dermis, which represents a large stem cell reservoir in the …

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Spatial Organization of Mesenchymal Stem Cells In Vitro—Results from a New Individual Cell-Based Model with Podia

Martin H K Hoffmann, Jens‐Peer Kuska, Matthias Zscharnack, Markus W. Loeffler et autres

Therapeutic application of mesenchymal stem cells (MSC) requires their extensive in vitro expansion. MSC in culture typically grow to confluence within a few weeks. They show spindle-shaped fibroblastoid morphology and align to each other in characteristic spatial patterns at high cell density. …

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Matrix-Associated Implantation of Predifferentiated Mesenchymal Stem Cells Versus Articular Chondrocytes

Bastian Marquaß, R. Schulz, Pierre Hepp, Matthias Zscharnack et autres

BACKGROUND: The use of predifferentiated mesenchymal stem cells (MSC) leads to better histological results compared with undifferentiated MSC in sheep. This raises the need for a longer term follow-up study and comparison with a clinically established method. HYPOTHESIS: We hypothesized that chondrogenic …

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Repair of Chronic Osteochondral Defects Using Predifferentiated Mesenchymal Stem Cells in an Ovine Model

Matthias Zscharnack, Pierre Hepp, Robert H. H. Richter, Thomas G Aigner et autres

BACKGROUND: The use of mesenchymal stem cells (MSCs) to treat osteochondral defects caused by sports injuries or disease is of particular interest. However, there is a lack of studies in large-animal models examining the benefits of chondrogenic predifferentiation in vitro for repair …

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Mesenchymal Stem Cells in Cartilage Repair: State of the Art and Methods to monitor Cell Growth, Differentiation and Cartilage Regeneration

Joerg Galle, A. Bader, Pierre Hepp, Wolfgang Grill et autres

Degenerative joint diseases caused by rheumatism, joint dysplasia or traumata are particularly widespread in countries with high life expectation. Although there is no absolutely convincing cure available so far, hyaline cartilage and bone defects resulting from joint destruction can be treated today …

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A novel MSC‐seeded triphasic construct for the repair of osteochondral defects

Bastian Marquaß, Jeremy S. Somerson, Pierre Hepp, Thomas G Aigner et autres

Mesenchymal stem cells (MSC) are increasingly replacing chondrocytes in tissue engineering based research for treatment of osteochondral defects. The aim of this work was to determine whether repair of critical-size chronic osteochondral defects in an ovine model using MSC-seeded triphasic constructs would …

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Impact of oxygen environment on mesenchymal stem cell expansion and chondrogenic differentiation

Axel Krinner, Matthias Zscharnack, A. Bader, Dirk Drasdo et autres

INTRODUCTION: In vitro expansion and differentiation of mesenchymal stem cells (MSC) rely on specific environmental conditions, and investigations have demonstrated that one crucial factor is oxygen environment. OBJECTIVES: In order to understand the impact of oxygen tension on MSC culture and chondrogenic …

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Low Oxygen Expansion Improves Subsequent Chondrogenesis of Ovine Bone-Marrow-Derived Mesenchymal Stem Cells in Collagen Type I Hydrogel

Matthias Zscharnack, C. Poesel, Joerg Galle, A. Bader

BACKGROUND/OBJECTIVE: A crucial factor when investigating cartilage tissue engineering using mesenchymal stem cells (MSCs) is their application in large-animal models and preclinical trials. However, in vitro studies using cells of these model organisms must proceed. Considering that oxygen tension is an important …

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Analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS

Beate Fuchs, Jürgen Schiller, Rosmarie Süß, Matthias Zscharnack et autres

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Cartilage tissue engineering by collagen matrix associated bone marrow derived mesenchymal stem cells.

R. Schulz, Matthias Zscharnack, I. Hanisch, M Geiling et autres

In this study we compared the in vitro formation of cartilaginous grafts composed of collagen type I hydrogel with both ovine primary articular chondrocytes (AC) and bone marrow derived mesenchymal stem cells (MSC). During 4 weeks of culture, aggregate properties were quantitatively …