Ali Ertürk

Creating bottom-up RNA transfer vehicles from synthetic protein assemblies

Maren Kirstin Schuhmacher, Christoph Gruber, Christopher M. R. Lang, Ricardo M. W. Ruijpers et autres

Abstract Evolution guides biological systems to populate ecological niches, with viruses among the most successful examples of this principle. Viruses evolved over billions of years to efficiently transfer genetic information. Although viruses are highly diverse, most have converged towards remarkable similarity in …

de, ch, gb (code pays fourni par la source)

Whole-mouse immunolabeling at cellular resolution for comprehensive 3D atlases

Hongcheng Mai, Yuewei Wang, Yu Michael Zhu, Yin Zhao et autres

cn, us, de, tr (code pays fourni par la source)

De novo H3.3K27M-altered diffuse midline glioma in human brainstem organoids to dissect GD2 CAR T cell function

Nils Bessler, Amber K. L. Wezenaar, Hendrikus C. R. Ariese, Celina Honhoff et autres

Diffuse midline glioma (DMG) is a highly aggressive and untreatable pediatric cancer primarily arising in the pontine brainstem region, necessitating the development of representative models for treatment advance. Here we developed an FGF4-driven human brainstem organoid model, which we used to genetically …

nl, dk, de, it, es (code pays fourni par la source)

Author Correction: Neuroimmune cardiovascular interfaces control atherosclerosis

Sarajo Kumar Mohanta, Li Peng, Yuanfang Li, Shu Lu et autres

de, cn, it, pl, se, gb, nl, ch, fr (code pays fourni par la source)

The stroke risk gene Foxf2 maintains brain endothelial cell function via Tie2 signaling

Katalin Völgyi, Judit González-Gallego, Stephan A. Müller, Mihail Ivilinov Todorov et autres

Cerebral small vessel disease (SVD) is a common chronic cerebrovascular disorder with poorly understood pathomechanisms. Genetic studies have identified FOXF2 as a major risk gene for both SVD and stroke. FOXF2 encodes a transcription factor primarily expressed in brain pericytes and endothelial …

de, it, se, us (code pays fourni par la source)

A fully iPS-cell-derived 3D model of the human blood–brain barrier for exploring neurovascular disease mechanisms and therapeutic interventions

Judit González-Gallego, Katalin Völgyi, Stephan A. Müller, Sophie Antesberger et autres

Blood-brain barrier (BBB) integrity is critical for brain homeostasis, with malfunctions contributing to neurovascular and neurodegenerative disorders. Mechanistic studies on BBB function have been mostly conducted in rodent and in vitro models, which recapitulate some disease features, but have limited translatability to …

de, tr, us, gb (code pays fourni par la source)

DISCO-seq: 3D single-cell transcriptomics of intact biological systems

Harsharan S. Bhatia, Laurent H. A. Simons, Louis Benedikt Kuemmerle, Cristin McCabe et autres

Single-cell transcriptomics has transformed tissue analysis, yet current methods struggle to integrate whole-tissue 3D architecture. Conventional techniques restrict molecular profiling to pre-selected 2D sections, losing systemic context and introducing anatomical bias by sampling less than 0.001% of a whole organism. To overcome …

de, us, gb (code pays fourni par la source)

A novel tamoxifen-inducible Mct8-CreERT2 mouse model for targeted studies of Mct8-expressing cells and thyroid hormone transport and function

Anna Molenaar, Noémi Mallet, Marin Bralo, Luciano Hoeher et autres

Deficiency of the Monocarboxylate Transporter 8 (MCT8) severely impairs thyroid hormone (TH) transport into the brain, disrupting brain development as well as peripheral TH homeostasis. Studies assessing MCT8 expression patterns and tissue-specific pathologies induced by local TH-deficiency are often inconclusive due to …

de, tr (code pays fourni par la source)

TMOD-24. Modeling Pediatric DMG in Human BrOs (Brainstem-regionalized Organoids) Dissects Tumour–Microglia–Immune Crosstalk and CAR T Cell Dysfunction

Nils Bessler, Amber K. L. Wezenaar, Hendrikus C. R. Ariese, Celina Honhoff et autres

Abstract Diffuse Midline Glioma (DMG) is a uniformly fatal paediatric brain tumour, most commonly arising in the pons, where access to tissue and effective therapies remain critically limited. To address this, we developed a novel guided human Brainstem-regionalized Organoid (BrO) model using …

nl, dk, us, de, it, es (code pays fourni par la source)

Leonardo: a toolset to correct sample-induced artifacts in light sheet microscopy images

Yu Liu, Lennart Kowitz, Tomáš Chobola, Kurt R. Weiss et autres

Abstract Selective plane illumination microscopy (SPIM, also known as light sheet fluorescence microscopy) is the method of choice for studying morphogenesis and function in biological specimens over extended periods, as it permits gentle and rapid volumetric imaging. In inhomogeneous samples, however, sample-induced …

de, us (code pays fourni par la source)

Graph neural networks learn emergent tissue properties from spatial molecular profiles

Mayar Ali, Sabrina Richter, Ali Ertürk, David S. Fischer et autres

Tissue phenotypes, such as metabolic states, inflammation, and tumor properties, emerge from both molecular states and spatial cell organization. Spatial molecular assays provide an unbiased view of tissue architecture, enabling phenotype prediction. Graph neural networks (GNNs) offer a natural framework for analyzing …

de, us (code pays fourni par la source)

LipiGo: A Versatile DNA-Lipid Nanoparticle Hybrid for Precision Drug Delivery

Karoline Kadletz, Ceren Kımna, Valentin Kenneth Reichenbach, Olga Carofiglio et autres

Abstract Lipid nanoparticles (LNPs) are established carriers for nucleic acid delivery, however, achieving efficient delivery to non-hepatic tissues remains a major challenge. Here, we present LipiGo, a nanocarrier platform engineered by integrating short single stranded DNA molecules into the lipid nanoparticle structure. …

de, se (code pays fourni par la source)