Shih-Cheng Chen

A Bright Ratiometric Dipyrene Probe for Functional Imaging of Condensate Microenvironments in Living Cells

Koki Matsumoto, Sora Kitai, Yoshio Nishiyama, Shogo Amemori et autres

Biomolecular condensates exhibit spatially heterogeneous microenvironments shaped by intertwined physicochemical factors, yet practical small-molecule tools for mapping these states in living cells remain limited. Here, we report PyLUMI, a bright dipyrene ratiometric probe, optimized by linker engineering to improve fluorescence output under …

jp, tw, us (code pays fourni par la source)

Coordinated translation initiation determines ‐1 programmed ribosomal frameshifting efficiency of chromosomal genes to impact on cell fitness

Sih‐Yun Tai, Masayuki Hashimoto, Yi‐Fang Zhuang, Hao‐Wen Liang et autres

The conserved -1 programmed ribosomal frameshifting (-1PRF), essential for eubacteria and RNA viruses, is thought to be exclusively regulated during translation elongation. One of the two required -1PRF cis-acting elements is a frameshifting stimulatory element (FSE), which pauses the elongating ribosome to …

tw, vn, nl (code pays fourni par la source)

Author response for "Coordinated translation initiation determines -1 programmed ribosomal frameshifting efficiency of chromosomal genes to impact on cell fitness"

Sih‐Yun Tai, Masayuki Hashimoto, Yi‐Fang Zhuang, Hao‐Wen Liang et autres

Data Resource Profile: Harmonized Electronic Health Records from the National Biobank Consortium of Taiwan (NBCT)

Yi‐Hsin Yang, Yung-Hsin Lee, Hsiu-An Lee, Li-Hsin Chang et autres

Taiwan announced the Human Biobank Management Act in 2010 [1] to establish a legal and ethical foundation for storing, managing, and utilizing participants’ biospecimens and health data. Over the years, many hospitals and research institutes have established biobanks, allowing secondary use for …

tw (code pays fourni par la source)

Author response for "Coordinated translation initiation determines -1 programmed ribosomal frameshifting efficiency of chromosomal genes to impact on cell fitness"

Sih‐Yun Tai, Masayuki Hashimoto, Yi‐Fang Zhuang, Hao‐Wen Liang et autres

Characterization of the binding features between SARS-CoV-2 5’-proximal transcripts of genomic RNA and nucleocapsid proteins

Shih-Cheng Chen, Cui-Ting Xu, Chuan-Fu Chang, Chia‐Shin Yang et autres

Packaging signals (PSs) of coronaviruses (CoVs) are specific RNA elements recognized by nucleocapsid (N) proteins that direct the selective packaging of genomic RNAs (gRNAs). These signals have been identified in the coding regions of the nonstructural protein 15 (Nsp 15) in CoVs …

tw (code pays fourni par la source)

Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a system

Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng‐Yao Chen et autres

Minus 1 programmed ribosomal frameshifting (−1 PRF) is a conserved translational regulation event essential for critical biological processes, including the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication. Efficient trans -modulation of the structured RNA element crucial to −1 PRF will endow …

tw (code pays fourni par la source)

Optimization of 5′UTR to evade SARS-CoV-2 Nonstructural protein 1-directed inhibition of protein synthesis in cells

Shih-Cheng Chen, Cui-Ting Xu, Chuan-Fu Chang, Ting-Yu Chao et autres

tw (code pays fourni par la source)

An iminocoumarin based covalent-assembly red-emitting fluorescent probe for detection of β-galactosidase activity in ovarian cancer cells

Ya‐Chu Ku, Pin‐Han Lin, Chung‐Yu Huang, Chia‐Wei Lee et autres

tw (code pays fourni par la source)

Structural phylogenetic analysis reveals lineage-specific RNA repetitive structural motifs in all coronaviruses and associated variations in SARS-CoV-2

Shih-Cheng Chen, René C. L. Olsthoorn, Chien‐Hung Yu

Abstract In many single-stranded (ss) RNA viruses, the cis-acting packaging signal that confers selectivity genome packaging usually encompasses short structured RNA repeats. These structural units, termed repetitive structural motifs (RSMs), potentially mediate capsid assembly by specific RNA–protein interactions. However, general knowledge of …

tw, nl (code pays fourni par la source)

Quantitative analysis of progesterone using isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry as a reference procedure for radioimmunoassay

Ming‐Hui Yang, Shih-Cheng Chen, Ko–Chin Chen, Han-Ping You et autres

tw, sg (code pays fourni par la source)

Relevance of the iron-responsive element (IRE) pseudotriloop structure for IRP1/2 binding and validation of IRE-like structures using the yeast three-hybrid system

Shih-Cheng Chen, René C. L. Olsthoorn

nl (code pays fourni par la source)