Daniele Pollini

Disruption of ribosome dynamics and mRNA homeostasis triggers a cascading imbalance in protein synthesis in models of Amyotrophic Lateral Sclerosis

Fabio Lauria, Federica Maniscalco, Cecilia Perrucci, Marta Marchioretto et autres

Abstract The RNA-binding protein TDP-43 is a major contributor and a pathological hallmark of Amyotrophic Lateral Sclerosis (ALS), yet how TDP-43 dysregulation mechanistically alters protein synthesis across neuronal compartments and disease models remains unclear. Here, we dissected TDP-43–driven translational alterations in both …

it (code pays fourni par la source)

Induction of Drug-Resistance and Production of a Culture Medium Able to Induce Drug-Resistance in Vinblastine Untreated Murine Myeloma Cells

Valentina Laghezza Masci, Davide Stefanoni, Angelo D’Alessandro, Marta Zambelli et autres

Cancer therapies use different compounds of synthetic and natural origin. However, despite some positive results, relapses are common, as standard chemotherapy regimens are not fully capable of completely eradicating cancer stem cells. While vinblastine is a common chemotherapeutic agent in the treatment …

it, us (code pays fourni par la source)

C9orf72 ALS/FTD dipeptide repeat protein levels are reduced by small molecules that inhibit PKA or enhance protein degradation

Nausicaa Valentina Licata, Riccardo Cristofani, Sally E. Salomonsson, Katherine Wilson et autres

Intronic GGGGCC (G4C2) hexanucleotide repeat expansion within the human C9orf72 gene represents the most common cause of familial forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) (C9ALS/FTD). Repeat‐associated non‐AUG (RAN) translation of repeat‐containing C9orf72 RNA results in the production of …

it, gb, us (code pays fourni par la source)

Multilayer and MATR3-dependent regulation of mRNAs maintains pluripotency in human induced pluripotent stem cells

Daniele Pollini, Rosa Loffredo, Federica Maniscalco, Marina Cardano et autres

Matrin3 (MATR3) is a nuclear RNA/DNA-binding protein that plays pleiotropic roles in gene expression regulation by directly stabilizing target RNAs and supporting the activity of transcription factors by modulating chromatin architecture. MATR3 is involved in the differentiation of neural cells, and, here, …

it (code pays fourni par la source)

MATR3-Dependent Multilayer Regulation of OCT4, NANOG and LIN28A is Essential for the Maintenance of the Human Pluripotency

Daniele Pollini, Rosa Loffredo, Annalisa Rossi, Mariachiara Micaelli et autres

it (code pays fourni par la source)

Generation and characterization of a human iPSC line from an ALS patient carrying the Q66K-MATR3 mutation

Daniele Pollini, Rosa Loffredo, Marina Cardano, Luciano Conti et autres

Fibroblasts isolated from an Amyotrophic Lateral Sclerosis (ALS)-patient carrying a mutation in Matrin-3 (p.Q66K -MATR3) gene were reprogrammed to the pluripotency stage by using non-integrating episomal plasmids. We generated the Q66K#44DRM induced pluripotent stem cell (iPSC) line that showed regular karyotype, expressed …

it (code pays fourni par la source)