K Andělová

First Dimension Trap‐and‐Elute Combined with Second Dimension Reversed‐Phase Liquid Chromatography Separation Using a Two‐Dimensional‐Liquid Chromatography‐Tandem Mass Spectrometry System for Sensitive Quantification of Human Insulin and Six Insulin Analogs in Plasma: Improved Chromatographic Resolution and Stability Testing

Pavel Šištík, Romana Uřinovská, Klára Handlosová, Petr Handlos et autres

Multidimensional chromatography coupled to tandem mass spectrometry (MS/MS), including simple sample preparation with protein precipitation, anion conversion with ammonium hydroxide, and solid-phase extraction using mixed-mode anion exchange in a 96-well plate format, has been validated for rapid simultaneous analysis of human insulin …

cz (code pays fourni par la source)

Contribution of methamphetamine and insulin to the death of a woman suffering from type I diabetes – which played the greater role?

Klára Handlosová, Pavel Šištík, Matěj Uvíra, K Andělová et autres

This report presents a fatal case of a young female Type I diabetic patient who developed convulsions and loss of consciousness after taking methamphetamine and spending some time in a dance club. During the convulsions, she was given sugar and when no …

cz (code pays fourni par la source)

P025: Comprehensive care improves the prognosis of women with severe HELLP syndrome and postpartal thrombotic microangiopathic syndrome

J Gumulec, Ondřej Šimetka, David Kaspřák, Erika Doležálková et autres

Comparison of a new immunoassay and PCR-based method for quantification of microRNAs in whole blood. A pilot methodical study

David Stejskal, Martina Hlozankova, Radka Šigutová, K Andělová et autres

BACKGROUND: MicroRNAs (miRNAs) are new generation biomarkers used in oncology, cardiology, metabolic syndrome, obesity or in neurology. miRNAs are short non-coding RNA molecules that regulate gene expression in eukaryotes. AIM: To compare a new commercial method for establishing miRNA (imunoassay) with a …

cz (code pays fourni par la source)

Gestational Diabetes – Metabolic Risks of Adult Women With Respect to Birth Weight

Daniela Vejražková, Olga Bradnová, Gabriela Vacínová, Josef Včelák et autres

Metabolic disorders such as obesity, insulin resistance and other components of metabolic syndrome (MetS) are connected with birth weight. Low and high birth weight is associated with a higher risk of developing type 2 diabetes mellitus, the mechanism is not clear. In …

cz (code pays fourni par la source)

Why should we accept the new international criteria for the diagnosis of gestational diabetes? (article in Czech)

Hana Krejčí, Kateřina Anderlová, K Andělová

Proč bychom měli přijmout nová mezinárodní kritéria pro diagnózu gestačního diabetes mellitus

Hana Krejčí, Kateřina Anderlová, K Andělová

Since 2008 there have been screened all pregnant women for gestational diabetes in the Czech Republic. Nevertheless since 2014 the results of the oral glucose tolerance test (which is a part of the screening) have been evaluated by two different sets of …

SAT0025 Pregnancy Outcomes in A Cohort of Women with Sle. Long Term 20 Years Observation

Dana Tegzová, K Andělová, I. Kučerová, J Madár et autres

cz, us, bd (code pays fourni par la source)

MTNR1B Genetic Variability Is Associated with Gestational Diabetes in Czech Women

Daniela Vejražková, Petra Lukášová, MarkéŽta Vaňkov‡á, Josef Včelák et autres

The gene MTNR1B encodes a receptor for melatonin. Melatonin receptors are expressed in human β-cells, which implies that genetic variants might affect glucose tolerance. Meta-analysis confirmed that the rs10830963 shows the most robust association. The aim of the study was to assess …

cz (code pays fourni par la source)

T2D Risk Haplotypes of the TCF7L2 Gene in the Czech Population Sample: the Association With Free Fatty Acids Composition

Josef Včelák, Daniela Vejražková, MarkéŽta Vaňkov‡á, Petra Lukášová et autres

The association of transcription factor 7-like 2 (TCF7L2) gene variants with the pathogenesis of T2D, gestational diabetes and polycystic ovary syndrome (PCOS) was examined. The study involved 1460 individuals: 347 T2D patients (D); 261 gestational diabetics (G); 147 offspring of T2D (O); …

cz (code pays fourni par la source)

O49 The pregnancy outcome in patients with systemic connective tissue disease

K Andělová, I. Kučerová, Dana Tegzová

Screening of Mutations and Polymorphisms in the Glucokinase Gene in Czech Diabetic and Healthy Control Populations

Petra Lukášová, Josef Včelák, MarkéŽta Vaňkov‡á, Daniela Vejražková et autres

Glucokinase (GCK) plays a key role in glucose metabolism. GCK mutations are known as a pathogenic cause of maturity-onset diabetes of the young type 2 (MODY2). These mutations are also found in gestational diabetics. The aim of our study was to assess …

cz, by (code pays fourni par la source)