Virneliz Fernández Vega

DDEL-18. High throughput screening driven personalized chemotherapy for brain metastases enabled by laser interstitial thermotherapy

Anjali Chauhan, Grace E. Hey, Nesrine Benslimane, Michael D. Cameron et autres

Abstract BACKGROUND Treatment of malignant brain tumors is limited in part because most chemotherapeutic agents do not cross the blood-brain barrier (BBB). Laser interstitial thermotherapy (LITT) transiently disrupts the BBB, potentially enhancing drug delivery. High-throughput screening (HTS) of tumor tissue enables identification …

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Multimodal Treatment of a Peripheral Primitive Neuroectodermal Tumor Originating From the Thoracic Cavity in a Dog

Leah H. Ackerman, Madison Toonder, Sarah Bosch, Varvara B. Semenova et autres

Peripheral primitive neuroectodermal tumor (pPNET) is a very rare, highly malignant tumor encountered in young dogs, with only four necropsy cases in the veterinary literature. A 1.5-year-old male intact French Bulldog presented for evaluation of progressive left forelimb lameness and ipsilateral Horner's …

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NVTG-08 PERSONALIZED TREATMENT OF MELANOMA BRAIN METASTASES THROUGH HIGH THROUGHPUT DRUG SCREENING

Bently Patrick Doonan, Timothy Spicer, Maryam Rahman, Louis D. Scampavia et autres

Abstract Melanoma is an increasing clinical concern, with an expected increase in incidence over the next 20 years. Compounding this problem, as many as 70% of all metastatic patients will develop brain metastases as a manifestation of their disease. Current strategies including …

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Abstract 3234: A new vulnerability to BET inhibition due to enhanced autophagy in BRCA2-deficient pancreatic cancer

Suyakarn Archasappawat, Eunjung Lee, Keely Y. Ji, Jocelyn V. Pena et autres

Abstract Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related deaths in the United States, with the lowest five-year survival rate of all cancers. Around 10% of pancreatic cancer diagnoses are thought to be familial pancreatic cancer (FPC). BRCA2 pathogenic …

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Rebamipide and Derivatives are Potent, Selective Inhibitors of Histidine Phosphatase Activity of the Suppressor of T Cell Receptor Signaling Proteins

Faisal Aziz, Kanamata Reddy, Virneliz Fernández Vega, Raja Dey et autres

The suppressor of T cell receptor signaling (Sts) proteins are negative regulators of immune signaling. Genetic inactivation of these proteins leads to significant resistance to infection. From a 590,000 compound high-throughput screen, we identified the 2-( 1H )-quinolinone derivative, rebamipide, as a …

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Protocol for high throughput 3D drug screening of patient derived melanoma and renal cell carcinoma

Luis M. Ortiz Jordan, Virneliz Fernández Vega, Justin Shumate, A. Mihaljević Peleš et autres

High Throughput Screening (HTS) with 3D cell models is possible thanks to the recent progress and development in 3D cell culture technologies. Results from multiple studies have demonstrated different drug responses between 2D and 3D cell culture. It is now widely accepted …

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Protocol for 3D screening of lung cancer spheroids using natural products

Virneliz Fernández Vega, Dong Joo Yang, Luis Ortiz Jordán, Fei Ye et autres

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer and accounts for ∼84% of all lung cancer cases. NSCLC remains one of the leading causes of cancer-associated death, with a 5-year survival rate less than 25%. This type …

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A Novel 3D Culture System for High-Throughput Hepatoxicity Screening.

Timothy Spicer, Virneliz Fernández Vega, Louis D. Scampavia, Lynsey Willetts et autres

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Figure 7, RecBCD nuclease assay

TD Bannister, Reji Nair, Timothy Spicer, Virneliz Fernández Vega et autres

Figure 8, AddAB nuclease assay

TD Bannister, Reji Nair, Timothy Spicer, Virneliz Fernández Vega et autres

Figure 5, One Step Synthesis of ML328

TD Bannister, Raveendranath Unnikrishnan Nair, Timothy Spicer, Virneliz Fernández Vega et autres

ML328: A Novel Dual Inhibitor of Bacterial AddAB and RecBCD Helicase-nuclease DNA Repair Enzymes

TD Bannister, Reji Nair, Timothy Spicer, Virneliz Fernández Vega et autres

The AddAB and RecBCD helicase-nucleases are related bacterial enzyme complexes that are instrumental in the repair of DNA double-strand breaks and in genetic recombination. Although they have been extensively studied both genetically and biochemically, no inhibitors specific for this class of enzymes …

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