Andres Jäschke

RiboGRAM learns multi-scale non-coding RNA grammar for structural and regulatory inference

wang hui, Zhaoming Chen, He Huang, Andres Jäschke et autres

Protein-coding sequences are read through the triplet genetic code, whereas non-coding RNA sequences encode function through motifs, their positioning, and base-paired architectures. Here, we identify a non-coding RNA grammar defined by multi-scale motif usage and 5'-to-3' positional gradients, and develop RiboGRAM to …

cn (code pays fourni par la source)

Fully Reversible Regulation of 8‐17 DNAzyme Activity Using Nucleoside‐Based Diarylethenes

Jörn Bargstedt, Simon M. Büllmann, Sophia Conwell, Andres Jäschke

ABSTRACT Controlling enzyme activity with external triggers is a central goal in chemical biology. Light is an especially attractive stimulus because it is noninvasive, offers high spatiotemporal precision, and can reversibly modulate molecular function. In this study, we use nucleoside‐based diarylethenes (DAEs) …

de (code pays fourni par la source)

A Near-Infrared Aptamer:Dye System For Live-Cell Super-Resolution RNA Imaging

Simon Fürbacher, Pia Doll, JingYe Zhang, Laura Lange et autres

Despite their advantageous properties for live-cell imaging and super-resolution microscopy, high-performance silicon rhodamine (SiR) near-infrared (NIR) probes are still rarely employed in RNA imaging via fluorescent light-up aptamers (FLAPs). Here, we developed the SiRiuS:SiR-5 system through a combined evolutionary approach: evolving the …

de (code pays fourni par la source)

HELIOS NAD-Seq: A Next-Generation Capture and Sequencing Protocol for NAD-Capped RNAs with Superior Targeting and Processing

Marvin Möhler, Tae Wha Seong, Frederik Weber, Héctor Morales Filloy et autres

de (code pays fourni par la source)

Co-evolution of a near-infrared aptamer:dye system for live-cell super-resolution RNA imaging

Jing‐ye Zhang, Laura Lange, Niklas van den Bergh, Yaqing Zhang et autres

ABSTRACT Despite their advantageous properties for live-cell imaging and super-resolution microscopy, high-performance silicon rhodamine (SiR) near-infrared (NIR) probes are still rarely employed in RNA imaging via fluorescent light-up aptamers (FLAPs). Here, we developed the SiRiuS:SiR-5 system through a combined evolutionary approach: evolving …

de (code pays fourni par la source)

Decoding the RNA binding systems by UltraGen

Yaqing Zhang, Hui Wang, Zhaoming Chen, Wenjun Lin et autres

de, cn (code pays fourni par la source)

Single-step discovery of high-affinity RNA ligands by UltraSelex

Yaqing Zhang, Yuan Ying Jiang, David Kuster, Qiwei Ye et autres

de, cn, us (code pays fourni par la source)

Flavin adenine dinucleotide (FAD) as a non-canonical RNA cap: Mechanisms, functions, and emerging insights

Pablo Gonzalez-Jabalera, Andres Jäschke

Flavin adenine dinucleotide (FAD), a versatile metabolic cofactor, is emerging as an important non-canonical RNA cap across various life domains. This review explores FAD's dual role as a coenzyme and an RNA modifier, focusing on its incorporation as a 5' cap structure …

de (code pays fourni par la source)

Super‐Resolved Protein Imaging Using Bifunctional Light‐Up Aptamers

Franziska Grün, Niklas van den Bergh, Maja Klevanski, Mrigank Singh Verma et autres

Abstract Efficient labeling methods for protein visualization with minimal tag size and appropriate photophysical properties are required for single‐molecule localization microscopy (SMLM), providing insights into the organization and interactions of biomolecules in cells at the molecular level. Among the fluorescent light‐up aptamers …

de, nl, us (code pays fourni par la source)

Super‐Resolved Protein Imaging Using Bifunctional Light‐Up Aptamers

Franziska Grün, Niklas van den Bergh, Maja Klevanski, Mrigank Singh Verma et autres

Efficient labeling methods for protein visualization with minimal tag size and appropriate photophysical properties are required for single-molecule localization microscopy (SMLM), providing insights into the organization and interactions of biomolecules in cells at the molecular level. Among the fluorescent light-up aptamers (FLAPs) …

de, nl, us (code pays fourni par la source)

Synthesis and Development of Inverse‐Type Nucleoside Diarylethene Photoswitches

Christoph M. Hendrich, Martin Reinschmidt, Simon M. Büllmann, Theresa Kolmar et autres

Nucleosidic diarylethenes (DAEs) have evolved from an emerging class of photochromes into a well-established option for integrating photochromic functionalities into biological systems. However, a comprehensive understanding of how chemical structure influences their photochromic properties remains essential. While structural features, such as an …

de (code pays fourni par la source)

Decoding the RNA interactome by UltraGen

Yaqing Zhang, Hui Wang, Zhaoming Chen, Wenjun Lin et autres

de, cn (code pays fourni par la source)