Neil P. Blackledge

Nuclear mTOR-Polycomb synergism at developmental gene promoters prone to activation

Persia Akbari Omgba, Gunwant Patil, Maximilian Stötzel, Chieh-Yu Cheng et autres

ABSTRACT Pluripotent cells hold the capacity to differentiate into cells with diverse functions, enabling development and regeneration. The balance between local epigenetic repression and maintaining the potential for prompt transcriptional activation supports pluripotency while allowing imminent differentiation. How the activation of developmental …

de, gb (code pays fourni par la source)

Epi-microRNA mediated metabolic reprogramming counteracts hypoxia to preserve affinity maturation

Rinako Nakagawa, Miriam Llorian, Sunita Varsani-Brown, Probir Chakravarty et autres

To increase antibody affinity against pathogens, positively selected GC-B cells initiate cell division in the light zone (LZ) of germinal centers (GCs). Among these, higher-affinity clones migrate to the dark zone (DZ) and vigorously proliferate by utilizing energy provided by oxidative phosphorylation …

gb, us (code pays fourni par la source)

The PNUTS phosphatase complex controls transcription pause release

Jessica R. Kelley, Emilia Dimitrova, Maciej Maciuszek, Hieu Nguyen et autres

Gene expression is regulated by controlling distinct steps of the transcriptional cycle, including initiation, pausing, elongation, and termination. Kinases phosphorylate RNA polymerase II (RNA Pol II) and associated factors to control transitions between these steps and to act as central gene regulatory …

gb, us (code pays fourni par la source)

The Polycomb system sustains promoters in a deep OFF state by limiting pre-initiation complex formation to counteract transcription

Aleksander Szczurek, Emilia Dimitrova, Jessica R. Kelley, Neil P. Blackledge et autres

The Polycomb system has fundamental roles in regulating gene expression during mammalian development. However, how it controls transcription to enable gene repression has remained enigmatic. Here, using rapid degron-based depletion coupled with live-cell transcription imaging and single-particle tracking, we show how the …

gb (code pays fourni par la source)

Epi-microRNA mediated metabolic reprogramming ensures affinity maturation

Rinako Nakagawa, Miriam Llorian, Sunita Varsani-Brown, Probir Chakravarty et autres

To increase antibody affinity against pathogens, positively selected GC-B cells initiate cell division in the light zone (LZ) of germinal centres (GCs). Among those, higher-affinity clones migrate to the dark zone (DZ) and vigorously proliferate by relying on oxidative phosphorylation (OXPHOS). However, …

gb, us (code pays fourni par la source)

A CpG island-encoded mechanism protects genes from premature transcription termination

Amy L. Hughes, Aleksander Szczurek, Jessica R. Kelley, Anna Lastuvkova et autres

Transcription must be tightly controlled to regulate gene expression and development. However, our understanding of the molecular mechanisms that influence transcription and how these are coordinated in cells to ensure normal gene expression remains rudimentary. Here, by dissecting the function of the …

gb (code pays fourni par la source)

A CpG island-encoded mechanism protects genes from premature transcription termination

Amy L. Hughes, Aleksander Szczurek, Jessica R. Kelley, Anna Lastuvkova et autres

Abstract Transcription must be highly controlled to regulate gene expression and development. However, our understanding of the molecular mechanisms that influence transcription and how these are coordinated in cells to ensure normal gene expression remains rudimentary. Here, we reveal that actively transcribed …

gb (code pays fourni par la source)

Variant PCGF1-PRC1 links PRC2 recruitment with differentiation-associated transcriptional inactivation at target genes

Hiroki Sugishita, Takashi Kondo, Shinsuke Ito, Manabu Nakayama et autres

Polycomb repressive complexes-1 and -2 (PRC1 and 2) silence developmental genes in a spatiotemporal manner during embryogenesis. How Polycomb group (PcG) proteins orchestrate down-regulation of target genes upon differentiation, however, remains elusive. Here, by differentiating embryonic stem cells into embryoid bodies, we …

jp, gb (code pays fourni par la source)

The molecular principles of gene regulation by Polycomb repressive complexes

Neil P. Blackledge, Robert J. Klose

gb (code pays fourni par la source)

BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome

Nadezda A. Fursova, Anne H. Turberfield, Neil P. Blackledge, Emma L. Findlater et autres

Histone-modifying systems play fundamental roles in gene regulation and the development of multicellular organisms. Histone modifications that are enriched at gene regulatory elements have been heavily studied, but the function of modifications found more broadly throughout the genome remains poorly understood. This …

gb (code pays fourni par la source)

Getting under the skin of Polycomb-dependent gene regulation

Neil P. Blackledge, Robert J. Klose

The Polycomb repressive system functions through chromatin to regulate gene expression and development. In this issue of Genes & Development, Cohen and colleagues (pp. 354–366) use the developing mouse epidermis as a model system to show that the two central Polycomb repressive …

gb (code pays fourni par la source)

BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome

Nadezda A. Fursova, Anne H. Turberfield, Neil P. Blackledge, Emma L. Findlater et autres

Abstract Histone-modifying systems play fundamental roles in gene regulation and the development of multicellular organisms. Histone modifications that are enriched at gene regulatory elements have been heavily studied, but the function of modifications that are found more broadly throughout the genome remains …

gb (code pays fourni par la source)