Accès ouvert
2025
article
OpenAlex
Constantine N. Tzouanas, Jessica E.S. Shay, Marc S. Sherman, Adam J. Rubin et autres
During chronic stress, cells must support both tissue function and their own survival. Hepatocytes perform metabolic, synthetic, and detoxification roles, but chronic nutrient imbalances can induce hepatocyte death and precipitate metabolic dysfunction-associated steatohepatitis (MASH, formerly NASH). Despite prior work identifying stress-induced drivers …
us
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Accès ouvert
2024
article
OpenAlex
Nuo Liu, Walaa E. Kattan, Benjamin E. Mead, Conner Kummerlowe et autres
High-throughput phenotypic screens using biochemical perturbations and high-content readouts are constrained by limitations of scale. To address this, we establish a method of pooling exogenous perturbations followed by computational deconvolution to reduce required sample size, labor and cost. We demonstrate the increased …
us
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Accès ouvert
2024
article
OpenAlex
Jakob Benedict Seidelin, Mariana Bronze, Anja Poulsen, Mohamed Attauabi et autres
The study reveals the regulatory dynamics of epithelial regeneration and extracellular matrix remodeling after experimental injury of the human colon in vivo and shows that human intestinal regeneration is different from data obtained from animals. A lag phase in epithelial restitution is …
dk, us
(code pays fourni par la source)
Accès ouvert
2023
preprint
OpenAlex
Constantine N. Tzouanas, Marc S. Sherman, Jessica E.S. Shay, Adam J. Rubin et autres
Abstract Under chronic stress, cells must balance competing demands between cellular survival and tissue function. In metabolic dysfunction-associated steatotic liver disease (MASLD, formerly NAFLD/NASH), hepatocytes cooperate with structural and immune cells to perform crucial metabolic, synthetic, and detoxification functions despite nutrient imbalances. …
us
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Accès ouvert
2023
preprint
OpenAlex
Benjamin E. Mead, Conner Kummerlowe, Nuo Liu, Walaa E. Kattan et autres
High-throughput phenotypic screens leveraging biochemical perturbations, high-content readouts, and complex multicellular models could advance therapeutic discovery yet remain constrained by limitations of scale. To address this, we establish a method for compressing screens by pooling perturbations followed by computational deconvolution. Conducting controlled …
us
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Accès ouvert
2022
article
OpenAlex
Sarah K. Nyquist, Patricia Gao, Tessa K. J. Haining, Michael R. Retchin et autres
Human breast milk (hBM) is a dynamic fluid that contains millions of cells, but their identities and phenotypic properties are poorly understood. We generated and analyzed single-cell RNA-sequencing (scRNA-seq) data to characterize the transcriptomes of cells from hBM across lactational time from …
us, pl
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Accès ouvert
2022
article
OpenAlex
Benjamin E. Mead, Kazuki Hattori, Lauren Levy, Shinya Imada et autres
The cellular composition of barrier epithelia is essential to organismal homoeostasis. In particular, within the small intestine, adult stem cells establish tissue cellularity, and may provide a means to control the abundance and quality of specialized epithelial cells. Yet, methods for the …
us
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Accès ouvert
2021
preprint
OpenAlex
Sarah K. Nyquist, Patricia Gao, Tessa K. J. Haining, Michael R. Retchin et autres
ABSTRACT Human breast milk is a dynamic fluid that contains millions of cells, but their identities and phenotypic properties are poorly understood. We used single-cell RNA-seq (scRNA-seq) to characterize the transcriptomes of cells from human breast milk (hBM) across lactational time from …
us
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Accès ouvert
2021
erratum
OpenAlex
Alex S. Genshaft, Carly G.K. Ziegler, Constantine N. Tzouanas, Benjamin E. Mead et autres
The original version of this Article contained an error in Fig. 3C furthest right panel, in which two white dots were inadvertently introduced. This has been corrected in both the PDF and HTML versions of the Article.
us, ca
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Accès ouvert
2021
article
OpenAlex
Alex S. Genshaft, Carly G.K. Ziegler, Constantine N. Tzouanas, Benjamin E. Mead et autres
A cell's phenotype and function are influenced by dynamic interactions with its microenvironment. To examine cellular spatiotemporal activity, we developed SPACECAT-Spatially PhotoActivatable Color Encoded Cell Address Tags-to annotate, track, and isolate cells while preserving viability. In SPACECAT, samples are stained with photocaged …
us, ca
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Accès ouvert
2021
article
OpenAlex
Jakob Benedict Seidelin, Martin Iain Bahl, Tine Rask Licht, Benjamin E. Mead et autres
BACKGROUND AND AIMS: The trigger hypothesis opens the possibility of anti-flare initiation therapies by stating that ulcerative colitis (UC) flares originate from inadequate responses to acute mucosal injuries. However, experimental evidence is restricted by a limited use of suitable human models. We …
dk, us
(code pays fourni par la source)
Accès ouvert
2020
preprint
OpenAlex
Benjamin E. Mead, Kazuki Hattori, Lauren Levy, Marko Vukovic et autres
Summary Barrier tissue epithelia play an essential role in maintaining organismal homeostasis, and changes in their cellular composition have been observed in multiple human diseases. Within the small intestinal epithelium, adult stem cells integrate diverse signals to regulate regeneration and differentiation, thereby …
us, fr, de, ru
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