Ying Lai

Organizing Large GTPase Atlastin‐Mediated Membrane Fusion by Programmable DNA Origami Platforms

Jingyao Li, Keying Wang, Qian Shi, Jiaqi Fan et autres

ABSTRACT In eukaryotic cells, the endoplasmic reticulum (ER) utilizes membrane fusion to form interconnected networks. GTP hydrolysis‐triggered conformational changes in ER‐anchored atlastins (ATLs) have been shown to be responsible for this process, which is facilitated by the amphipathic helix within its C‐terminal …

cn (code pays fourni par la source)

Calcium-sensitive synaptotagmin 11-lipid interaction modulates exo-endocytosis

Xuanang Wu, Jingyu Yao, Jingxiao Huo, Shaoqin Hu et autres

Synaptotagmins (Syts) are the primary Ca2+-sensors for synaptic vesicle exocytosis, while most mammalian Syts are non-Ca2+-affinitive and play critical roles in neurotransmission and synaptic plasticity with unclear mechanisms. Here, we show that high-alkaline non-Ca2+-binding Syt11 exhibits higher affinity for acidic phospholipids and …

cn, tw (code pays fourni par la source)

Munc18 modulates syntaxin phase separation to promote exocytosis

Qibing Pei, Qixin Chen, Zhiqi Tian, Le Zhu et autres

cn, us (code pays fourni par la source)

Synaptotagmin-1 serves as a primary Zn2+ sensor to mediate spontaneous neurotransmitter release under pathological conditions

Yijuan Xiang, Lele Cui, Jingyu Yao, Xiaochu Lou et autres

In the pre-synapse, vesicle fusion is a crucial process for neurotransmitter release in the nervous system. Under physiological conditions, synaptotagmin-1 (Syt1) locks synaptic vesicles in a priming state, allowing them to undergo synchronized neurotransmitter release upon Ca2+ activation. Elevation of intracellular ions …

cn, us (code pays fourni par la source)

Discovery of novel covalent PI3Kδ inhibitors bearing alaninamide moiety by lysine-targeted covalent strategy

Bo Yuan, Mengyan Ma, Yujie Wu, Jiaxin Liu et autres

cn (code pays fourni par la source)

Bioengineered nanovesicles for efficient siRNA delivery through ligand-receptor-mediated and enzyme-controlled membrane fusion

Lele Cui, Yongsheng Cui, Jing Liu, Wei Li et autres

A major obstacle in knocking down oncogenes for tumor therapy is the efficient delivery of siRNA into the cytosolic spaces of cancer cells. Here, we genetically bioengineer biomimetic nanovesicles with tumor-recognition and enzyme-controlled membrane fusion functions for efficiently delivering small interfering RNA …

cn (code pays fourni par la source)

Lysine-Targeted Covalent Strategy Leading to the Discovery of Novel Potent PROTAC-Based PI3Kδ Degraders

Bo Yuan, Jiaxin Liu, Yujie Wu, Mengyao Chen et autres

Proteolysis-targeting chimera (PROTAC) technology was employed to achieve the degradation of PI3Kδ in this study, and a series of PROTAC-based PI3Kδ degraders were first developed. Lysine-targeted covalent strategy led to the discovery of novel potent PROTAC-based PI3Kδ degraders. After screening and structure–activity …

cn (code pays fourni par la source)

Data from: Synaptotagmin-1 serves as a primary Zn2+ sensor to mediate spontaneous neurotransmitter release under pathological conditions

Yijuan Xiang, Lele Cui, Jingyu Yao, H. Lu et autres

This dataset provides the source data for the paper titled "Synaptotagmin-1 serves as a primary Zn²⁺ sensor to mediate spontaneous neurotransmitter release under pathological conditions." Data includes electrophysiological patch-clamp recordings of miniature excitatory postsynaptic currents (mEPSCs), spontaneous EPSCs (sEPSCs), and evoked EPSCs, …

cn, us (code pays fourni par la source)

Corrigendum to “STING guides the STX17-SNAP29-VAMP8 complex assembly to control autophagy” [Cell Insight 3 (2024) 100147]

Xiaoyu Song, Yufeng Xi, Ming Dai, Tao Li et autres

[This corrects the article DOI: 10.1016/j.cellin.2024.100147.].

cn, us (code pays fourni par la source)

Cryo-EM reveals mechanisms of natural RNA multivalency

Liu Wang, Jiahao Xie, Tao Gong, You‐Liang Tu et autres

Homo-oligomerization of biological macromolecules leads to functional assemblies that are critical to understanding various cellular processes. However, RNA quaternary structures have rarely been reported. Comparative genomics analysis has identified RNA families containing hundreds of sequences that adopt conserved secondary structures and likely …

cn, us, hk (code pays fourni par la source)

Discovery of Novel Covalent Pi3kδ Inhibitors Bearing Alaninamide Moiety by Lysine Targeting Covalent Strategy

Bo Yuan, Mengyan Ma, Yujie Wu, Jiaxin Liu et autres

Synergistic regulation of fusion pore opening and dilation by SNARE and synaptotagmin-1

Kaiju Li, Kaiyu Li, Jiaqi Fan, Xing Zhang et autres

Fusion pore opening is a transient intermediate state of synaptic vesicle exocytosis, which is highly dynamic and precisely regulated by the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex and synaptotagmin-1 (Syt1). Yet, the regulatory mechanism is not fully understood. In this …

cn (code pays fourni par la source)