Francesco Secundo

Effects of Orange Peel-Derived Carbon Sources on Nannochloropsis salina Mixotrophic Cultivation

Carlo Esposito, Giancarlo Aldini, Yanan Yin, Stefano Gandolfi et autres

Microalgae are recognized as sustainable biofactories for metabolites relevant to nutraceutical, pharmaceutical, and marine drug applications. Nannochloropsis salina is notable for its production of bioactive lipids, including the pharmaceutically relevant omega-3 eicosapentaenoic acid (EPA). Here, we evaluated orange peel extract (OPE), a …

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Sustainable production strategies of omega-3 PUFAs and their enrichment in the human diet

Kunpeng Gao, X Li, Hailong Zhang, Jianan Sun et autres

With the continuing improvement of living standards, humans have been paying more attention to the influence of diet on their health and disease prevention. One group of the most important and essential foods are those rich in polyunsaturated fatty acids (PUFAs), in …

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Inhibitory effect of gut microbiota-fermented trisaccharide GuFGa (Glc-Fuc-Gal) on Campylobacter jejuni: insights from transcriptomic and metabolomic analyses

Xinmiao Ren, Shuang Li, C P Wang, Francesco Secundo et autres

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rDNA-mediated multicopy integration and gene dosage quantification system for microbial zeaxanthin biosynthesis

Ruijing Ling, Shuting Hou, Xiangzhao Mao, Francesco Secundo et autres

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Enhanced Intestinal Absorption of Proteins via a Natural Metabolite-Based Deep Eutectic System

Simiao Di, Jie Luo, Yongqiang Lian, Francesco Secundo et autres

A natural metabolite-based deep eutectic system composed of betaine and lactic acid (BL-DES) was developed as a food-grade strategy to enhance intestinal absorption of proteins. Insulin was selected as a model protein for convenient and reliable assessment of intestinal absorption. BL-DES reduced …

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Insights into the catalytic mechanism of formate dehydrogenases from different microbial sources

Laura Legnani, Marco Gargiulo, Elia Lio, Enrico Mario Alessandro Fassi et autres

Four formate dehydrogenases (FDHs) from Pseudomonas sp . 101, Myceliophthora thermophila , Chaetomium thermophilum , and Ogataea parapolymorpha were recombinantly produced, purified, and characterized to investigate their catalytic properties and reaction mechanisms. The enzymes were studied for their ability to oxidize formate …

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Decoding the Prolonged Suppression of Zn on Fermentative Hydrogen Generation from Sewage Sludge

Mingyang Liu, Xiaomin Li, Francesco Secundo, Chen Lyu et autres

The presence of zinc (Zn) in various wastes poses significant challenges to biohydrogen recovery from organic wastes. The inhibitory effect of Zn on hydrogen production from sewage sludge was investigated, and a pronounced inhibition on hydrogen production was observed across the tested …

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Smart Alginate–Chitosan Oligosaccharide Microspheres for Controlled Protein Release in the Gastrointestinal Tract with Superior Protein Protection

Simiao Di, Jie Luo, Francesco Secundo, Xiangzhao Mao et autres

The design of biopolymer-based carriers that protect proteins from gastrointestinal degradation while enabling controlled release is essential for advancing oral delivery systems. In this study, smart alginate–chitosan oligosaccharide (ALG–COS) microspheres were fabricated via an initial rapid divalent cation-mediated cross-linking followed by carbodiimide-mediated …

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Author response for "Insights into the catalytic mechanism of formate dehydrogenases from different microbial sources"

Laura Legnani, Marco Gargiulo, Elia Lio, Enrico Mario Alessandro Fassi et autres

Co-cultivation of Nannochloropsis salina and Xanthophyllomyces dendrorhous in mixed medium enhances lipid and antioxidant yields

Carlo Esposito, Elia Lio, Stefano Gandolfi, Gianluca Ottolina et autres

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Microalgae: versatile microbial factories for natural product synthesis

Elia Lio, Gioia Di Stefano, Jacopo Paini, Carlo Esposito et autres

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Characterization and antimicrobial activity of extracts from microalgae Chlorococcum sp. with green organic solvents

Elia Lio, Martina Dramis, Gianluca Ottolina, Giancarlo Aldini et autres

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