Nicolas Loof

Current and Future Challenges in Cytometry: Reports of the CYTO 2025 Conference Workshops

Paul K. Wallace, Evan R. Jellison, Sherry Thornton, Kathleen Kluepfel et autres

joint effort begun in 2018 and continued in 2019 by Kamila Czechowska proved to be a valuable reference [1,2].Building on that spirit, we present summaries from CYTO 2025, held in Denver, Colorado, from May 31 to June 4.This manuscript serves as a …

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Cyt‐Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2025 Conference Workshops

Paul K. Wallace, Evan R. Jellison, Sherry Thornton, Kathleen Kluepfel et autres

joint effort begun in 2018 and continued in 2019 by Kamila Czechowska proved to be a valuable reference [1,2].Building on that spirit, we present summaries from CYTO 2025, held in Denver, Colorado, from May 31 to June 4.This manuscript serves as a …

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CD11c + CD88 + CD317 + myeloid cells are critical mediators of persistent CNS autoimmunity

Navid Manouchehri, Rehana Z. Hussain, Petra D. Cravens, Ekaterina Esaulova et autres

Significance The bone marrow-derived CD11c + CD88 + CD317 + myeloid cells within the central nervous system are associated with clinical experimental autoimmune encephalomyelitis. Transcriptional analyses identify ITGAX- (CD11c), C5AR1- (CD88), and BST2- (CD317) expressing cells as a distinct myeloid subset in …

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CD11c + CD88 + CD317 + myeloid cells are critical mediators of persistent CNS autoimmunity

Navid Manouchehri, Rehana Z. Hussain, Petra D. Cravens, Brian T. Edelson et autres

Abstract Background Natalizumab, a humanized monoclonal antibody (mAb) against α4-integrin, reduces the number of dendritic cells (DC) in cerebral perivascular spaces in multiple sclerosis (MS). Selective deletion of α4-integrin in CD11c + cells should curtail their migration to the CNS and ameliorate …

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FCRG - Fixation and Sorting for RNA

Nicolas Loof, A Mehrnoosh, Alan J. Bergeron, Alan Saluk et autres

Increasingly, Flow Cytometry Shared Resource Facilities are asked to sort cells for RNA isolation either in bulk or at the single cell level. In many cases, the ability to fix the cell prior to sorting is desirable. With so many fixation methods …

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NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte

S. M. Nashir Udden, Youn-Tae Kwak, Victoria Godfrey, Md Abdul Wadud Khan et autres

Hepatocellular carcinoma (HCC) is a deadly human cancer associated with chronic inflammation. The cytosolic pathogen sensor NLRP12 has emerged as a negative regulator of inflammation, but its role in HCC is unknown. Here we investigated the role of NLRP12 in HCC using …

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Author response: NLRP12 suppresses hepatocellular carcinoma via downregulation of cJun N-terminal kinase activation in the hepatocyte

S. M. Nashir Udden, Youn-Tae Kwak, Victoria Godfrey, M.A. Wadud Khan et autres

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TLR 3 agonism re‐establishes CNS immune competence during α4‐integrin deficiency

Rehana Z. Hussain, Petra C. Cravens, Richard Doelger, Brianne Dentel et autres

Abstract Objective Natalizumab blocks α4‐integrin‐mediated leukocyte migration into the central nervous system (CNS). It diminishes disease activity in multiple sclerosis (MS), but carries a high risk of progressive multifocal encephalopathy (PML), an opportunistic infection with JV virus that may be prompted by …

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Defining standard enzymatic dissociation methods for individual brains and spinal cords in EAE

Rehana Z. Hussain, William A. Miller-Little, Richard Doelger, Gary Cutter et autres

Objective To determine the capacity, effectiveness, efficiency, and reliability of select tissue dissociation methods to isolate mononuclear cells from the CNS of mice with experimental autoimmune encephalomyelitis (EAE). Methods As part of an assay qualification, we tested the isolation method Percoll PLUS …

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OMIP‐040: Optimized gating of human prostate cellular subpopulations

Gervaise H. Henry, Nicolas Loof, Douglas W. Strand

THIS panel was optimized to quantify the relative frequency of the major cell types present in the human prostate in addition to their sorting for downstream applications.Tissue resident white blood cells (referred to here as leukocytes) are identified by CD45, epithelia are …

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P16-49. Broad types of cytokines secreted by Gag-specific T cells from HIV infected patients on HAART

Matthieu Montès, Nicolas Loof, Amanda J. Cobb, D Jutras et autres

Previously described highly immunogenic epitopes from Gag are able to stimulate Th1/CTL cells. This response is essential for controlling progression of HIV infection. Techniques like IFN-γ-ELISPOT and IFN-γ intracellular staining are currently the standard methods to define antigen-specific immune responses. A recent …

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P16-29. HIV Nef-specific T cells: Th1/CTL, Th2 and Th17 responses

Matthieu Montès, Nicolas Loof, Amanda J. Cobb, D Jutras et autres

Identification of promiscuous antigenic regions is crucial for the design of an epitope-based vaccine. Still, the quality of the responses has been under estimated in most cases when only IFN-γ is used to measure anti-HIV cellular immunity. Luminex multiplexing system allows the …

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