Isabelle Huvent

Simultaneous Visualization of Distinct Posttranslational Modification States of β‐Catenin Using Genetic Code Expansion and Click Chemistry

Christer Abou Anny, Sébastien Nouaille, Isabelle Huvent, Corentin Spriet et autres

A novel strategy based on genetic code expansion combined with click chemistry for the simultaneous visualization of distinct posttranslational modification (PTM) states of a single protein within living cells. As a model, it is focused on threonine 41 (T41) of β-catenin, a …

fr (code pays fourni par la source)

Glycan-mediated adhesion mechanisms in antibiotic-resistant bacteria

Clara Dessenne, Christophe Mariller, Olivier Vidal, Isabelle Huvent et autres

Bacterial adhesins play a central role in host-pathogen interactions, with many specifically targeting glycans to mediate bacterial colonization, influence infection dynamics, and evade host immune responses. In this review, we focus on bacterial pathogens identified by the World Health Organization as critical …

fr, jp (code pays fourni par la source)

A Step‐by‐Step Guide for the Production of Recombinant Fluorescent TAT‐HA‐Tagged Proteins and their Transduction into Mammalian Cells

Christer Abou Anny, Sébastien Nouaille, Régis Fauré, Céline Schulz et autres

Investigating the function of target proteins for functional prospection or therapeutic applications typically requires the production and purification of recombinant proteins. The fusion of these proteins with tag peptides and fluorescently derived proteins allows the monitoring of candidate proteins using SDS-PAGE coupled …

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Cyclophilin A allows the allosteric regulation of a structural motif in the disordered domain 2 of NS5A and thereby fine-tunes HCV RNA replication

Marie Dujardin, Vanesa Madan, Neha S. Gandhi, François‐Xavier Cantrelle et autres

Implicated in numerous human diseases, intrinsically disordered proteins (IDPs) are dynamic ensembles of interconverting conformers that often contain many proline residues. Whether and how proline conformation regulates the functional aspects of IDPs remains an open question, however. Here, we studied the disordered …

fr, de, au (code pays fourni par la source)

F2‐06‐01: MAJOR DIFFERENCES BETWEEN THE SELF‐ASSEMBLY, SEEDING BEHAVIOR, AND INTERACTION WITH MODULATORS OF HEPARIN‐INDUCED VERSUS IN‐VITRO PHOSPHORYLATED TAU

Gal Bitan, Clément Despres, Jing Di, François‐Xavier Cantrelle et autres

Self-assembly of tau into neurotoxic oligomers and fibrils, and cell-to-cell spreading of these pathological tau species are critical processes underlying the pathogenesis of Alzheimer's disease (AD) and other tauopathies. Modulating the self-assembly process and inhibiting formation and spreading of such toxic species …

us, fr, de (code pays fourni par la source)

Major Differences between the Self-Assembly and Seeding Behavior of Heparin-Induced and in Vitro Phosphorylated Tau and Their Modulation by Potential Inhibitors

Clément Despres, Jing Di, François‐Xavier Cantrelle, Zizheng Li et autres

Self-assembly of the microtubule-associated protein tau into neurotoxic oligomers, fibrils, and paired helical filaments, and cell-to-cell spreading of these pathological tau species are critical processes underlying the pathogenesis of Alzheimer's disease and other tauopathies. Modulating the self-assembly process and inhibiting formation and …

fr, us, in, cn, de (code pays fourni par la source)

The Neuronal Tau Protein Blocks In Vitro Fibrillation of the Amyloid-β (Aβ) Peptide

Cecilia Wallin, Yoshitaka Hiruma, Sebastian K.T.S. Wärmländer, Isabelle Huvent et autres

se, nl, fr, ch (code pays fourni par la source)

Two Tau binding sites on tubulin revealed by thiol-disulfide exchanges

Marlène Martinho, Diane Allegro, Isabelle Huvent, Charlotte Chabaud et autres

Tau is a Microtubule-associated protein that induces and stabilizes the formation of the Microtubule cytoskeleton and plays an important role in neurodegenerative diseases. The Microtubules binding region of Tau has been determined for a long time but where and how Tau binds …

fr (code pays fourni par la source)

The Neuronal Tau Protein Blocks in Vitro Fibrillation of the Amyloid-β (Aβ) Peptide at the Oligomeric Stage

Cecilia Wallin, Yoshitaka Hiruma, Isabelle Huvent, Jüri Jarvet et autres

In Alzheimer’s disease, amyloid-β (Aβ) plaques and tau neurofibrillary tangles are the two pathological hallmarks. The co-occurrence and combined reciprocal pathological effects of Aβ and tau protein aggregation have been observed in animal models of the disease. However, the molecular mechanism of …

se, nl, fr, ch (code pays fourni par la source)

Identification of the Tau phosphorylation pattern that drives its aggregation

Clément Despres, Cillian Byrne, Haoling Qi, François‐Xavier Cantrelle et autres

Determining the functional relationship between Tau phosphorylation and aggregation has proven a challenge owing to the multiple potential phosphorylation sites and their clustering in the Tau sequence. We use here in vitro kinase assays combined with NMR spectroscopy as an analytical tool …

fr (code pays fourni par la source)

Glycan Determinants of Heparin-Tau Interaction

Jing Zhao, Isabelle Huvent, Guy Lippens, David Eliezer et autres

us, fr, cn (code pays fourni par la source)

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

Clément Danis, Clément Despres, Luiza M. Bessa, Idir Malki et autres

Aggregates of the neuronal Tau protein are found inside neurons of Alzheimer's disease patients. Development of the disease is accompanied by increased, abnormal phosphorylation of Tau. In the course of the molecular investigation of Tau functions and dysfunctions in the disease, nuclear …

fr (code pays fourni par la source)