Flavia Bazzoni

MiR-146b restoration in T-large granular lymphocyte leukemia: Molecular insights for a new therapeutic strategy

Antonella Teramo, Elisa Rampazzo, Eleonora Roncaglia, Barbara Mariotti et autres

Abstract INTRODUCTION T-Large Granular Lymphocyte Leukemia (T-LGLL) is a rare lymphoproliferative disorder characterized by the clonal expansion of cytotoxic LGLs. The disease exhibits significant heterogeneity, with clinical manifestations ranging from indolent to life-threatening conditions. T-LGL lymphoproliferation is driven by both somatic gain-of-function …

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Transcriptomic landscape of CD8+ and CD4 + T-LGL leukemia revealed the distinct impact of STAT3 and STAT5B activating mutations

Giulia Calabretto, Andrea Binatti, Antonella Teramo, Alessia Buratin et autres

The biological basis of the high clinical heterogeneity of T-LGL Leukemia (T-LGLL) is not completely understood and effective therapies for this disease are lacking. Through RNA-Sequencing of purified T-LGLs we reveal gene expression profiles and pathway dysregulations in the major patient subgroups, …

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Uncovering common transcriptional features shared by mature peripheral blood PMN-MDSCs and tumor-infiltrating neutrophils in humans

Chiara Lattanzi, Francisco M. Bianchetto-Aguilera, Marta Donini, Francesca Pettinella et autres

Human polymorphonuclear-myeloid-derived suppressor cells (PMN-MDSCs) consist of circulating low-density neutrophils (LDNs) characterized by the ability to inhibit T-cell responses. In previous studies, we demonstrated that the mature fraction of PMN-MDSCs (i.e. mPMN-MDSCs) exerts more potent immunosuppressive functions than its immature counterpart. More …

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Innate immune reprogramming in circulating neutrophils of COPD patients

Barbara Mariotti, Chiara Bracaglia, Sara Gasperini, Giulia Sartori et autres

Background Chronic obstructive pulmonary disease (COPD) involves both local and systemic neutrophilic inflammation, with dysregulation in blood neutrophil numbers, frequencies, and functions. Objective We sought to characterize the transcriptional and epigenetic profiles of circulating neutrophils in patients with COPD and explore correlations …

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Cell damage shifts the microRNA content of small extracellular vesicles into a Toll-like receptor 7-activating cargo capable to propagate inflammation and immunity

Valentina Salvi, Carolina Gaudenzi, Barbara Mariotti, Gaia Giongrandi et autres

The physiological relevance of cell-to-cell communication mediated by small extracellular vesicle-encapsulated microRNAs (sEV-miRNAs) remains debated because of the limiting representativity of specific miRNAs within the extracellular pool. We hypothesize that sEV-miRNA non-canonical function consisting of the stimulation of Toll-like receptor 7 (TLR7) …

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Activating STAT3 Mutations Drive CD38 Expression in T-Large Granular Lymphocytic Leukemia

Giulia Calabretto, Elena Buson, Zachary Hutchins, Colleen Isabelle et autres

The tumor-specific expression of CD38 is a crucial prognostic marker and a key therapeutic target in various hematologic malignancies. However, the regulatory mechanisms of CD38 expression in malignant cells are not well understood. This study identifies a cohort of T-cell large granular …

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Surface CD52, CD84, and PTGER2 mark mature PMN-MDSCs from cancer patients and G-CSF-treated donors

Francesca Pettinella, Barbara Mariotti, Chiara Lattanzi, Kirsten Bruderek et autres

Precise molecular characterization of circulating polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) is hampered by their mixed composition of mature and immature cells and lack of specific markers. Here, we focus on mature CD66b + CD10 + CD16 + CD11b + PMN-MDSCs (mPMN-MDSCs) from …

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Implementation of a combined bioinformatics and experimental approach to address lncRNA mechanism of action: The example of NRIR

Barbara Mariotti, Costanza Di Blas, Flavia Bazzoni

In this study, we demonstrate the benefit of applying combined strategies to analyze lncRNA action based on bioinformatics and experimental information. This strategy was developed to identify the molecular function of negative regulator of interferon response (NRIR), a type I interferon-stimulated gene …

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Impact of Sex on Circulating Leukocytes Composition in COPD Patients

Natalia Troianova, Barbara Mariotti, Valentina Micheletti, Federica Calzetti et autres

PURPOSE: Chronic obstructive pulmonary disease is characterized by chronic inflammatory response both at the lung site and at the systemic level. Abnormalities in circulating leukocytes have been reported to occur in COPD patients and have been often shown to correlate with the …

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Characterization of Long Non-Coding RNAs in Systemic Sclerosis Monocytes: A Potential Role for PSMB8-AS1 in Altered Cytokine Secretion

Nila H. Servaas, Barbara Mariotti, Maarten van der Kroef, Catharina G. K. Wichers et autres

Systemic sclerosis (SSc) is a chronic autoimmune disease mainly affecting the connective tissue. In SSc patients, monocytes are increased in circulation, infiltrate affected tissues, and show a pro-inflammatory activation status, including the so-called interferon (IFN) signature. We previously demonstrated that the dysregulation …

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Editorial: Regulation of Soluble Immune Mediators by Non-Coding RNAs

Daniela Bosisio, Flavia Bazzoni

EDITORIAL article Front. Immunol., 15 October 2020Sec. Cytokines and Soluble Mediators in Immunity Volume 11 - 2020 | https://doi.org/10.3389/fimmu.2020.607222

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Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR

Nicola Tamassia, Marco Antonio Cassatella, Flavia Bazzoni

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