Uncovering common transcriptional features shared by mature peripheral blood PMN-MDSCs and tumor-infiltrating neutrophils in humans
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Le résumé fourni par la source
Human polymorphonuclear-myeloid-derived suppressor cells (PMN-MDSCs) consist of circulating low-density neutrophils (LDNs) characterized by the ability to inhibit T-cell responses. In previous studies, we demonstrated that the mature fraction of PMN-MDSCs (i.e. mPMN-MDSCs) exerts more potent immunosuppressive functions than its immature counterpart. More recently, we defined a specific gene signature of mPMN-MDSCs from cancer patients and G-CSF-treated donors (GDs) by bulk RNA sequencing (RNA-seq) experiments. In this study, by performing single-cell RNA-seq (scRNA-seq) experiments of circulating mPMN-MDSCs from non-small cell lung cancer (NSCLC) patients, we identified a major scRNA-seq cell cluster (arbitrarily named NSCLC c6) specifically displaying immunosuppressive and protumor transcriptomic features. Then, by analyzing publicly available scRNA-seq datasets from human tumor-associated neutrophils (TANs), we uncovered three TAN clusters (arbitrarily named TAN c6-c8) that were found to share with NSCLC c6 several common genes and transcription factor (TF) regulons associated with response to hypoxia, positive regulation of angiogenesis, and metabolic reprogramming. Furthermore, by performing scRNA-seq experiments of GD mPMN-MDSCs, we uncovered four scRNA-seq cell clusters (arbitrarily named GD c4-c7) that were enriched for the same genes and pathways characterizing NSCLC c6 and TAN c6-c8 cells. Altogether, these data uncover that human circulating mPMN-MDSCs and TANs from different cancer types share scRNA-seq cell clusters with transcriptomic similarities, supporting the notion that they might be strictly related.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Uncovering common transcriptional features shared by mature peripheral blood PMN-MDSCs and tumor-infiltrating neutrophils in humans
- Date Crossref
- 01/07/2025
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Verona Department of Medicine pays non établi dans la noticeUniversité ou école supérieure
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University Hospital Foundation pays non établi dans la noticeOrganisation à but non lucratif
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Essen University Hospital pays non établi dans la noticeOrganisme public
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Deutsches Konsortium für Translationale Krebsforschung pays non établi dans la noticeStructure de recherche
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University and Hospital Trust (AOUI) Transfusion Medicine Department pays non établi dans la noticeUniversité ou école supérieure
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Section of Hematology and Bone Marrow Transplant Unit pays non établi dans la noticeInstitution
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Department of Engineering for Innovation Medicine (DIMI) pays non établi dans la noticeInstitution
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Partner Site Essen-Düsseldorf pays non établi dans la noticeInstitution
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University Hospital Essen Department of Otorhinolaryngology pays non établi dans la noticeUniversité ou école supérieure
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German Cancer Consortium pays non établi dans la noticeInstitution
Department of Medicine — University of Verona, University Hospital Foundation et Essen University Hospital, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.