Accès ouvert
2026
preprint
OpenAlex
Laura Alunno, Ilaria Massignani, Meriem Hadjer Hamadou, Fabio Mazza et autres
Abstract To prioritize germline genetic variants affecting mRNA fate at the post-transcriptional and translational levels, we leveraged sucrose-gradient-based isolation of 80S monosomes and polysomes, followed by mRNA retrieval and paired-end sequencing. Total cytoplasmic RNA was also sequenced for comparison. Experiments were performed …
it
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Meriem Hadjer Hamadou, Laura Alunno, Daniele Peroni, Michael Pancher et autres
To search for genetic sources of allele-specific mRNA translation, we leveraged heterozygous polymorphisms and variants present in the exome of HCT116 colorectal adenocarcinoma-derived cells, computing allelic fractions from both total and polysome-associated RNA from RNA-Seq data. Allelic imbalance in polysomal RNA led …
it, us
(code pays fourni par la source)
Accès ouvert
2026
article
OpenAlex
Ágata Sofia Assunção Carreira, Marianna Ciuffreda, Nathakan Thongon, Charles M. Haughey et autres
Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme in the NAD + salvage pathway and a promising therapeutic target in cancer.Resistance to NAMPT inhibitors, such as FK866, remains a key limitation to their clinical translation.While acquired resistance in cancer cell lines has been …
it, gb
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Paolo Vincenzo Fioretti, Anna Barbieri, Alice Migazzi, Davide Bressan et autres
Chronic activation of glial cells leads to the dysfunction and degeneration of motor and cortical neurons in amyotrophic lateral sclerosis and frontotemporal dementia with an unknown mechanism. To shed light on the molecular pathogenetic processes underlying the exordium and contribution of gliosis …
it, us, gb, lb, es, bg, jp
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Fabio Lauria, Federica Maniscalco, Cecilia Perrucci, Marta Marchioretto et autres
Abstract The RNA-binding protein TDP-43 is a major contributor and a pathological hallmark of Amyotrophic Lateral Sclerosis (ALS), yet how TDP-43 dysregulation mechanistically alters protein synthesis across neuronal compartments and disease models remains unclear. Here, we dissected TDP-43–driven translational alterations in both …
it
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Ágata Sofia Assunção Carreira, Marianna Ciuffreda, Natthakan Thongon, Charles M. Haughey et autres
ABSTRACT Nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme in the NAD+ salvage pathway and a promising therapeutic target in cancer. Resistance to NAMPT inhibitors, such as FK866, remains a key limitation to their clinical translation. While acquired resistance in cancer cell lines …
it, gb
(code pays fourni par la source)
Accès ouvert
2025
preprint
OpenAlex
Meriem Hadjer Hamadou, Laura Alunno, Daniele Peroni, Michael Pancher et autres
Abstract To search for genetic sources of allele-specific mRNA translation, we leveraged heterozygous polymorphisms and variants present in the exome of HCT116-derived cell lines, computing allelic fractions in total and polysome-associated RNA from RNA-seq data. Allelic imbalance in polysomal RNA led us …
it
(code pays fourni par la source)
Accès ouvert
2025
article
OpenAlex
Jessica Corsi, Pouriya Sharbatian Semnani, Daniele Peroni, Romina Belli et autres
Extracellular vesicles (EVs) are cell-secreted membranous particles contributing to intercellular communication. Coding and noncoding RNAs can be detected as EV cargo, and RNA-binding proteins (RBPs), such as hnRNPA2B1, have been circumstantially implicated in EV-RNA sorting mechanisms. However, the contribution of competitive RBP-RNA …
it, de, us
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Tommaso Brighenti, Marco Mazzola, Gabriele Tomè, Mariella Scalfati et autres
BACKGROUND: The vitreous humor serves as a window into the physiological and pathological processes of the eye, particularly the retina. Diabetic retinopathy (DR), a leading cause of blindness, involves hyperglycemia-induced damage to retinal cells, leading to ischemia and elevated nitric oxide levels, …
it, us
(code pays fourni par la source)
Accès ouvert
2024
article
OpenAlex
Elena Gurrieri, Giulia Carradori, Michela Roccuzzo, Michael Pancher et autres
BACKGROUND: Extracellular vesicles (EVs) are cell-secreted particles conceived as natural vehicles for intercellular communication. The capacity to entrap heterogeneous molecular cargoes and target specific cell populations through EV functionalization promises advancements in biomedical applications. However, the efficiency of the obtained EVs, the …
it, nl
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Elena Gurrieri, Giulia Carradori, Michela Roccuzzo, Michael Pancher et autres
Abstract Extracellular vesicles (EVs) are cell-secreted particles conceived as natural vehicles for intercellular communication. The intrinsic biocompatibility, stability in biofluids, and heterogeneous molecular cargo of EVs promise advancements in targeted therapy applications. However, predicting cell-targeting spectrum and cargo delivery are fundamental challenges …
it, us, nl
(code pays fourni par la source)
Accès ouvert
2024
preprint
OpenAlex
Jessica Corsi, Daniele Peroni, Romina Belli, Michelangelo Lassandro et autres
Abstract Extracellular vesicles (EVs) are cell-secreted membranous particles contributing to intercellular communication. Coding and non-coding RNAs are widely detected EV cargo, and RNA-binding proteins (RBPs), such as hnRNPA2B1, have been circumstantially implicated in sorting vesicular RNAs. However, the contribution of competitive RBP-RNA …
it, de
(code pays fourni par la source)