Jörg Nickelsen

Conserved and lineage-specific roles of KEA-mediated ion homeostasis in Chlamydomonas.

Tobias Wunder, Leon Eulitz, Luis Kramer, Zahin Mohd Ali et autres

In Arabidopsis thaliana, seamless plastid gene expression and development depend on finely balanced ion homeostasis across the inner envelope (IE) membrane, maintained by the K+/H+ antiporters AtKEA1/2. To assess whether these functions are retained across mono- and polyplastidic representatives of the green …

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Cold stress impairs CurT accumulation and thylakoid architecture in Synechocystis sp. PCC 6803

Victoria J. C. Holzer, Anne-Christin Pohland, Jörg Nickelsen, Marcel Dann et autres

Low-temperature stress affects the thylakoid membrane system in both cyanobacteria and chloroplasts. In this study, a previously unrecognized link between cold-stress acclimation and the thylakoid-shaping CurT abundance in Synechocystis sp. PCC 6803 is identified. The results demonstrate that thylakoid architecture and photosynthetic …

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The toxic effects of meta‐tyrosine are related to its misincorporation into the proteome and to altered metabolism in cyanobacteria

Hagit Zer, Roei Matan, David Rasin, Yoram Soroka et autres

Cyanobacteria are prolific photosynthetic bacteria whose notable adaptability, coupled with a high metabolic versatility, enables them to thrive in diverse habitats across the globe. They play a key role in global primary production and nutrient cycling, but uncontrolled proliferation of certain species …

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Bioactivated scaffolds promote angiogenesis and lymphangiogenesis for dermal regeneration in vivo

Benedikt Fuchs, Sinan Mert, Daniel Hofmann, Constanze Kuhlmann et autres

Chronic wounds represent an unresolved medical challenge with significant impact for patients’ quality of life and global healthcare. Diverse in origin, ischemic-hypoxic and inflammatory conditions play central roles as pathological features that impede proper tissue regeneration. In this study, we propose an …

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The subcellular localisation of early photosystem II assembly in Synechocystis sp. PCC 6803

Matthias Ostermeier, Steffen Heinz, Jörg Nickelsen

The spatial organization of the de novo assembly of photosystem II (PSII) in cyanobacteria, particularly its subcellular starting point, is a matter of ongoing scientific debate. Here, we use fluorescence microscopy and biochemical experiments to demonstrate that the most likely marker of …

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Pilot system for engineering sustainable aquatic food webs: Utilizing cyanobacteria for continuous secretion of ω-polyunsaturated fatty acids

Maximilian K. Lübben, Steffen Heinz, Sabine Gießler, Martin Lehmann et autres

This study introduces a pilot system that uses genetically engineered and marker-less strains of Synechocystis sp.PCC 6803 for enhanced secretion of ω-polyunsaturated fatty acids (ω-PUFAs), specifically intended for aquaculture applications.By inactivating the native acyl-acyl carrier protein synthetase (Aas) and expressing the heterologous …

In Vivo Biocompatibility of Synechococcus sp. PCC 7002-Integrated Scaffolds for Skin Regeneration

Benedikt Fuchs, Sinan Mert, Constanze Kuhlmann, Alexandra Birt et autres

Cyanobacteria, commonly known as blue-green algae, are prevalent in freshwater systems and have gained interest for their potential in medical applications, particularly in skin regeneration. Among these, Synechococcus sp. strain PCC 7002 stands out because of its rapid proliferation and capacity to …

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Generation of photosynthetic biomaterials by loading electrospun fibres with the green microalgae, Chlamydomonas reinhardtii

Leah Williams, Victoria J. C. Holzer, Jörg Nickelsen, Fiona L. Hatton et autres

Hypoxic environments within tissues, characterised by low concentrations of oxygen, are a major limitation in certain biomedical applications such as wound healing and tissue engineering. Electrospun fibrous mats have demonstrated potential for use in these applications as they can absorb fluids and …

CLEM , a universal tool for analyzing structural organization in thylakoid membranes

Maximilian K. Lübben, Andreas Klingl, Jörg Nickelsen, Matthias Ostermeier

Chlorophyll (Chl) plays a crucial role in photosynthesis, functioning as a photosensitizer. As an integral component of this process, energy absorbed by this pigment is partly emitted as red fluorescence. This signal can be readily imaged by fluorescence microscopy and provides a …

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Structure, biogenesis, and evolution of thylakoid membranes

Matthias Ostermeier, Adriana Garibay‐Hernández, Victoria J. C. Holzer, Michael Schroda et autres

Cyanobacteria and chloroplasts of algae and plants harbor specialized thylakoid membranes (TMs) that convert sunlight into chemical energy. These membranes house PSII and I, the vital protein-pigment complexes that drive oxygenic photosynthesis. In the course of their evolution, TMs have diversified in …

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Biocompatibility of Synechococcus sp. PCC 7002 with Human Dermal Cells In Vitro

Benedikt Fuchs, Sinan Mert, Constanze Kuhlmann, Sara Taha et autres

Being the green gold of the future, cyanobacteria have recently attracted considerable interest worldwide. This study investigates the adaptability and biocompatibility of the cyanobacterial strain Synechococcus sp. PCC 7002 with human dermal cells, focusing on its potential application in biomedical contexts. First, …

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NTRC regulates CP12 to activate Calvin–Benson cycle during cold acclimation

Jing Tsong Teh, Verena Leitz, Victoria J. C. Holzer, Daniel Neusius et autres

NADPH-dependent thioredoxin reductase C (NTRC) is a chloroplast redox regulator in algae and plants. Here, we used site-specific mutation analyses of the thioredoxin domain active site of NTRC in the green alga Chlamydomonas reinhardtii to show that NTRC mediates cold tolerance in …

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