Michael Schroda

Trans-dimerization of Amyloid Precursor Protein Family Members Induces Pre- and Postsynaptic Differentiation through Distinct Signaling Pathways

Ritu Rajender, Natalie Foth, Simone Eggert, Sandra Schilling et autres

The amyloid precursor protein (APP), a key factor in Alzheimer's disease (AD) pathology, and its two mammalian homologs, amyloid precursor-like proteins 1 and 2 (APLP1 and APLP2), are considered as members of the synaptic adhesion molecule (SAM) family. They are localized to …

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Multi-knockout of 29 phytotoxic proteins and metabolites does not completely abolish virulence of the necrotrophic fungus Botrytis cinerea

Nassim Safari, Patrick Pattar, Marat Magomedov, Andrea Tobian Herreno et autres

Abstract Botrytis cinerea is a necrotrophic plant pathogen with an extremely wide host range. During invasion, the fungus induces rapid host cell death and proliferates in the necrotic tissue. Host killing involves secretion of lytic enzymes, phytotoxic metabolites and cell death inducing …

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Simple Electroporation of Chlamydomonas reinhardtii Strains with an Intact Cell Wall

Maximilian Meßmer, Félix de Carpentier, Ezekiel Lam, Meggie Hong et autres

Abstract Chlamydomonas reinhardtii is a model green alga extensively used to study photosynthesis and cilia using molecular biology and genetics. Electroporation is a very common technique to transform DNA into the nuclear genome, which is essential to generate mutant collections and express …

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Depletion of Chloroplast HSP70B Triggers Proteostasis Collapse and Compromises Thylakoid Membrane Integrity in Chlamydomonas

Anna Probst, Stefan Schmollinger, Jonas Berg, Ann-Katrin Unger et autres

Abstract Chloroplast HSP70 is an essential component of the plastid proteostasis network, supporting protein folding, complex assembly and disassembly, and stress acclimation. Despite extensive genetic evidence for its essentiality, the cellular consequences of reduced chloroplast HSP70 activity remain poorly defined. Here, we …

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Simple Electroporation of Chlamydomonas reinhardtii Strains With an Intact Cell Wall

Maximilian Meßmer, Félix de Carpentier, Ezekiel Lam, Meggie Hong et autres

strains with an intact cell wall. • Faster than currently available electroporation protocols.

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CurT/CURT1 proteins are involved in cell and chloroplast division coordination of cyanobacteria and green algae

Marcel Dann, Eunchul Kim, Konomi Fujimura‐Kamada, Vjosa Berisha et autres

Plant proteins of the CURVATURE THYLAKOID 1 (CURT1) family and their prokaryotic CurT homologues are key determinants of the three-dimensional structure of the thylakoid membrane systems in chloroplasts and cyanobacteria. As the evolutionary origin of the CURT1/CurT family appears to coincide with …

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The proxiomes of CDJ5 and PGRL1 in Chlamydomonas reinhardtii overlap depending on the ability of CDJ5 to bind a 4Fe-4S cluster

Katharina K oumlnig, Melissa Misir, Justus Niemeyer, Sarah Gabelmann et autres

The chloroplast chaperone HSP70B from Chlamydomonas reinhardtii works with the J-domain co-chaperones CDJ1 to CDJ6. CDJ1 delivers unfolded proteins, while CDJ2 delivers VIPP1 as substrate to HSP70B. CDJ3 to CDJ5 contain 4Fe-4S clusters in addition to the J domain, but their function …

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Internalization of affinity tags enables the purification of secreted Chlamydomonas proteins

Anna Probst, Doreen Knochenhauer, Justus Niemeyer, Michael Schroda

There is great interest in establishing microalgae as new platforms for the sustainable production of high-value products such as recombinant proteins. Many human therapeutic proteins must be glycosylated, which requires their passage through the secretory pathway into the culture medium. While the …

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Complexome profiling of the Chlamydomonas psb28 mutant reveals TEF5 as an early PSII assembly factor

Julia Lang, Katharina König, Benedikt Venn, Saskia Zeilfelder et autres

PSII assembly requires auxiliary factors, including Psb28. Although the absence of Psb28 in cyanobacteria has little effect on PSII assembly, we show here that the Chlamydomonas (Chlamydomonas reinhardtii) psb28 null mutant is severely impaired in PSII assembly, showing drastically reduced PSII supercomplexes, …

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Unfolded protein responses in Chlamydomonas reinhardtii

Sarah Gabelmann, Michael Schroda

Abstract The disruption of protein homeostasis leads to the increased un- and misfolding of proteins and the formation of toxic protein aggregates. Their accumulation triggers an unfolded protein response that is characterized by the transcriptional upregulation of molecular chaperones and proteases, and …

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Purification and Electron Transfer from Soluble c-Type Cytochrome TorC to TorA for Trimethylamine N-Oxide Reduction

Alka Panwar, Berta M. Martins, Frederik Sommer, Michael Schroda et autres

The enterobacterium Escherichia coli present in the human gut can reduce trimethylamine N-oxide (TMAO) to trimethylamine during anaerobic respiration. The TMAO reductase TorA is a monomeric, bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor-containing enzyme that belongs to the dimethyl sulfoxide reductase family of molybdoenzymes. …

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The cyanobacterial protein VIPP1 forms ESCRT-III-like structures on lipid bilayers

Sichen Pan, Karin Gries, Benjamin D. Engel, Michael Schroda et autres

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