Kenneth M. Pryse

Tension anisotropy drives fibroblast phenotypic transition by self-reinforcing cell–extracellular matrix mechanical feedback

Farid Alisafaei, Delaram Shakiba, Yuan Hong, Ghiska Ramahdita et autres

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Cell–matrix feedback controls stretch-induced cellular memory and fibroblast activation

Yuan Hong, Xiangjun Peng, Haomin Yu, Mohammad Jafari et autres

Mechanical stretch can activate long-lived changes in fibroblasts, increasing their contractility and initiating phenotypic transformations. This activation, critical to wound healing and procedures such as skin grafting, increases with mechanical stimulus for cells cultured in two-dimensional but is highly variable in cells …

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Virtual blebbistatin: A robust and rapid software approach to motion artifact removal in optical mapping of cardiomyocytes

Louis G. Woodhams, Jingxuan Guo, David Schuftan, John J. Boyle et autres

Fluorescent reporters of cardiac electrophysiology provide valuable information on heart cell and tissue function. However, motion artifacts caused by cardiac muscle contraction interfere with accurate measurement of fluorescence signals. Although drugs such as blebbistatin can be applied to stop cardiac tissue from …

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The mechanobiology of fibroblast activation

Yuan Hong, Peng Xiangjun, Haomin Yu, Mohammad Jafari et autres

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Matrix Softening Controls Stretch-Induced Cellular Memory and Fibroblast Activation

Hong Yuan, Xiangjun Peng, Haomin Yu, Mohammad Jafari et autres

Abstract Mechanical stretching of living tissues can activate long-lived changes in tissue cells such as fibroblasts, increasing their contractility and initiating phenotypic transformations. Increased mechanical stimulus typically leads to monotonically increasing activation of fibroblasts cultured in 2D, but activation levels are difficult …

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Tension anisotropy drives phenotypic transitions of cells via two-way cell-ECM feedback

Farid Alisafaei, Delaram Shakiba, Leanne E. Iannucci, Matthew D. Davidson et autres

Abstract Mechanical factors such as stress in the extracellular environment are known to affect phenotypic commitment of cells. However, the stress fields experienced by cells in tissues are multiaxial, and the ways that cells integrate this multiaxial information are largely unknown. Here, …

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The Balance between Actomyosin Contractility and Microtubule Polymerization Regulates Hierarchical Protrusions That Govern Efficient Fibroblast–Collagen Interactions

Delaram Shakiba, Farid Alisafaei, Alireza Savadipour, Roger A. Rowe et autres

Fibroblasts undergo a critical transformation from an initially inactive state to a morphologically different and contractile state after several hours of being embedded within a physiologically relevant three-dimensional (3D) fibrous collagen-based extracellular matrix (ECM). However, little is known about the critical mechanisms …

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Stable fitting of noisy stress relaxation data

Roger A. Rowe, Kenneth M. Pryse, Elliot L. Elson, Guy M. Genin

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Atomic Force Microscopy of Phase Separation on Ruptured, Giant Unilamellar Vesicles, and a Mechanical Pathway for the Co-Existence of Lipid Gel Phases

Yanfei Jiang, Kenneth M. Pryse, Srikanth Singamaneni, Guy M. Genin et autres

Giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs) are synthetic model systems widely used in biophysical studies of lipid membranes. Although SLBs are advantageous for biophysical analysis, phase separation behaviors of lipid species in these two model systems can differ due …

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Energy dissipation in quasi-linear viscoelastic tissues, cells, and extracellular matrix

Behzad Babaei, Aaron J. Velasquez-Mao, Kenneth M. Pryse, William B. McConnaughey et autres

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Atomic force microscopy of phase separation on ruptured, giant unilamellar vesicles

Yanfei Jiang, Guy M. Genin, Kenneth M. Pryse, Elliot L. Elson

Abstract Giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs) are synthetic model systems widely used in biophysical studies of lipid membranes. Phase separation behaviors of lipid species in these two model systems differ due to the lipid-substrate interactions that are present …

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Investigation of Nanoscopic Phase Separations in Lipid Membranes Using Inverse FCS

Yanfei Jiang, Kenneth M. Pryse, Artem Melnykov, Guy M. Genin et autres

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